@article{mbs:/content/journal/micro/10.1099/00221287-144-4-1013, author = "Gu, X. X. and Rossau, R. and Jannes, G. and Ballard, R. and Laga, M. and Van Dyck, E", title = "The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay", journal= "Microbiology", year = "1998", volume = "144", number = "4", pages = "1013-1019", doi = "https://doi.org/10.1099/00221287-144-4-1013", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-1013", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Haemophilus ducreyi", keywords = "chancroid", keywords = "rrs—rrl spacer", abstract = "The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.", }