1887

Abstract

The direction of growth of hyphae of the pathogenic fungus responds thigmotropically to surface contours by following scratches, ridges and grooves and by penetrating pores. Here it is shown that the thigmotropic response to ridges is attenuated by GdCl and verapamil [blockers of stretch-activated (SA) ion channels and L-type calcium channels, respectively]. At low concentrations, both compounds reduced the percentage of hyphae reorienting on contact with a ridge without markedly affecting hyphal extension rate, suggesting a possible role for SA or other calcium channels in the transduction of the thigmotropic response. In addition, patch-clamp recordings demonstrated SA channel activity in the plasma membrane of both yeast and hyphal cells of Two distinct SA channels with conductances of 54 pS and 20-25 pS in 200 mM KCI were observed in protoplasts from yeast cells and one channel of 51 pS was found in protoplasts from hyphal cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-3-689
1998-03-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/3/mic-144-3-689.html?itemId=/content/journal/micro/10.1099/00221287-144-3-689&mimeType=html&fmt=ahah

References

  1. Allen G.J., Sanders D. 1994; Two voltage-gated, calcium release channels coreside in the vacuolar membrane of broad bean guard cells.. Plant Cell 6:685–694
    [Google Scholar]
  2. Beck-Sagué C.M., Jarvis W.R. 1993; Secular trends in the epidemiology of nosocomial fungus infections in the United States, 1880-1990.. J Infect Dis 167:1247–1252
    [Google Scholar]
  3. Bertl A., Slayman C.L. 1992; Complex modulation of cation channels in the tonoplast and plasma membrane of Saccharo- myces cerevisiae: single channel studies.. J Exp Biol 172:271–287
    [Google Scholar]
  4. Ding J.P., Pickard B.G. 1993; Mechanosensory calcium- selective cation channels in epidermal cells.. Plant J 3:83–110
    [Google Scholar]
  5. Garrill A., Jackson S.L., Lew R.R., Heath I.B. 1993; Ion channels and tip growth: stretch activated channels generate a Ca2+ gradient essential for hyphal growth.. Eur J Cell Biol 60:358–365
    [Google Scholar]
  6. Gow N.A.R. 1995; Tip growth and polarity.. In The Growing Fungus pp. 277–299 Gow N.A.R., Gadd G.M. Edited by London: Chapman & Hall;
    [Google Scholar]
  7. Gow N.A.R. 1996; Candida albicans: morphogenesis and pathogenesis.. Jpn J Med Mycol 37:49–58
    [Google Scholar]
  8. Gow N.A.R., Perera T.H.S., Sherwood-Higham J., Gooday G.W., Gregory D.W., Marshall D. 1994; Contact sensing in Candida albicans: a possible aid to epithelial penetration.. J Med Vet Mycol 30:461–469
    [Google Scholar]
  9. Gray D.I., Morris B.M. 1992; A low cost video analysis system for the BBC Master computer.. Binary Comput Microbiol 4:58–61
    [Google Scholar]
  10. Gustin M.C., Zhou X.-L., Martinac B., Kung C. 1988; A mechanosensitive ion channel in the yeast plasma membrane.. Science 242:762–765
    [Google Scholar]
  11. Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J. 1981; Improved patch clamp technique for high resolution current recording from cells and cell-free membrane patches.. Pflueg Arch Eur J Physiol 391:85–100
    [Google Scholar]
  12. Hoch M.C., Staples R.C., Whitehead B., Comeau J., Wolf E.D. 1987; Signalling for growth orientation and cell differentiation by surface topography in Uromyces.. Science 235:1659–1662
    [Google Scholar]
  13. Jibiki M., Kuno Y., Shinoyama H. 1993; Isolation and properties of large cell strains from a methanol-utilizing yeast, Candida sp. N-16 by colchicine treatment.. J Gen Appl Microbiol 39:439–442
    [Google Scholar]
  14. John J.A., Quenouille M.M. 1977 Experiments: Design and Analysis, 2nd edn.. London: Charles Griffin;
    [Google Scholar]
  15. Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J. 1996; A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties.. J Biol Chem 271:4183–4187
    [Google Scholar]
  16. Levina N.N., Lew R.R., Hyde G.J., Heath I.B. 1995; Theroles of Ca2+ and plasma membrane ion channels in hyphal tip growth of Neurospora crassa.. J Cell Sci 108:3405–3417
    [Google Scholar]
  17. Martinac B., Beuchner M., Delcour A.H., Adler J., Kung C. 1987; Mechanosensitive ion channel in Escherichia coli.. Proc Natl Acad Sci USA 84:2297–2301
    [Google Scholar]
  18. Odds F.C. 1988 Candida and Candidosis, 2nd edn.. New York: Bailliere Tindall;
    [Google Scholar]
  19. Ray T.L., Payne C.D. 1988; Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase.. Infect Immun 56:1942–1949
    [Google Scholar]
  20. Read N.D., Kellock L.J., Knight H., Trewavas A.J. 1992; Contact sensing during infection by fungal pathogens.. In Perspectives in Plant Cell Recognition pp. 137–172 Callow J.A., Green J.R. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  21. Read N.D., Kellock L.J., Collins T.J., Gundlach A.M. 1997; Role of topography sensing for infection-structure differentiation in cereal rust fungi.. Planta 202:163–170
    [Google Scholar]
  22. Sackin H. 1995; Mechanosensitive channels.. Anna Rev Physiol 57:333–353
    [Google Scholar]
  23. Sherwood J., Gow N.A.R., Gooday G.W., Gregory D.W., Marshall D. 1992; Contact sensing in Candida albicans: a possible aid to epithelial penetration.. J Med Vet Mycol 30:461–469
    [Google Scholar]
  24. Thomine S., Zimmerman S., Vandujin B., Barbier-Brygoo H. 1994; Calcium channel antagonists induce direct inhibition of the outward rectifying potassium channel in tobacco protoplasts.. FEBS Lett 340:45–50
    [Google Scholar]
  25. Xiao J.-Z., Watanabe T., Kamakura T., Ohshima A., Yamaguchi I. 1994; Studies on cellular differentiation of Magnaporthe grisea. Physicochemical aspects of substratum surfaces in relation to appressorium formation.. Physiol Mol Plant Pathol 44:227–236
    [Google Scholar]
  26. Zhou X.-L., Kung C. 1992; A mechanosensitive ion channel in Schizosaccharomyces pombe.. EMBO J 11:2869–2875
    [Google Scholar]
  27. Zhou X.-L., Stumpf M.A., Hoch H.C., Kung C. 1991; Amechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.. Science 253:1415–1417
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-3-689
Loading
/content/journal/micro/10.1099/00221287-144-3-689
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error