1887

Abstract

ISA 1307 displays biphasic growth in a medium containing a mixture of glucose (0.5%, w/v) and acetic acid (0.5%, w/v), pH 5.0 and 3.0. In cells harvested during the first growth phase, no activity of a mediated acetic acid transport system was found. Incubation of these cells in phosphate buffer with cycloheximide for 1 h restored activity of an acetic acid carrier which behaved as the one present in glucose-grown cells. These results indicated that the acetic acid carrier is probably present in cells from the first growth phase of the mixed medium but its activity was affected by the presence of acetic acid in the culture medium. In glucose-grown cells, after incubation in phosphate buffer with glucose and acetic acid, the activity of the acetic acid carrier decreased significantly with increased acid concentration in the incubation buffer. At acid concentrations above 16.7 mM, no significant carrier activity was detectable. Furthermore, the intracellular acid concentration increased with the extracellular one and was inversely correlated with the activity of the acetic acid carrier, suggesting the involvement of a feedback inhibition mechanism in the regulation of the carrier. During biphasic growth, the first phase corresponded to a simultaneous consumption of glucose and acetic acid, and the second to the utilization of the remaining acid. The enzyme acetyl-CoA synthetase was active in both growth phases, even in the presence of glucose. Activity of isocitrate lyase and phosphoenolpyruvate carboxykinase was found only in acetic-acid-grown cells. Thus it appears that both membrane transport and acetyl-CoA synthetase and their regulation are important for to metabolize acetic acid in the presence of glucose. This fact correlates with the high resistance of this yeast to environments with mixtures of sugars and acetic acid such as those often present during wine fermentation.

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1998-03-01
2021-05-18
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References

  1. van den Berg M.A., Jong-Gubbels P., Kortland C.J., van Dijken J.P., Pronk J.T., Steensma H.Y. 1996; The two acetyl-CoA synthetases ofSaccharomyces cerevisiae differ in respect to kinetic properties and transcriptional regulation. J Biol Chem 46:28953–28959
    [Google Scholar]
  2. Casal M., Leáo C. 1995; Utilization of short-chain monocarboxylic acids by the yeastTorulaspora delbrueckii: specificity of the transport systems and their regulation. Biochim Biophys Acta 1267:122–130
    [Google Scholar]
  3. C#x00E1;ssio F., Le#x00E1;o C., van Uden N. 1987; Transport of lactate and other short-chain monocarboxylates in the yeastSaccharomyces cerevisiae. . Appl Environ Microbiol 53:509–513
    [Google Scholar]
  4. C#x00E1;ssio F., C#x0242;rte-Real M., Le#x00E1;o C. 1993; Quantitative analysis of proton movements associated with the uptake of weak carboxylic acids. The yeastCandida utilis as a model. Biochim Biophys Acta 1153:59–66
    [Google Scholar]
  5. Furhman G.F., V#x00D6;lker B. 1992; Regulation of glucose transport inSaccharomyces cerevisiae. . J Biotechnol 27:1–15
    [Google Scholar]
  6. Grenson M. 1992; Amino acid transporters in yeast: structure. function and regulation.. In Molecular Aspects of Transport Proteins, pp. 219–245 De Pont. J. J. H. H. M. Edited by Amsterdam:: Elsevier.;
    [Google Scholar]
  7. Hor#x00E1;k J. 1986; Amino acid transport in eucaryotic microorganisms. Biochim Biophys Acta 864:223–256
    [Google Scholar]
  8. Huismank LA., Hansen T.A. 1982; Induction of isocitrate lyase inEscherichia coli. . In Sourcebook of Experiments for the Teaching of Microbiology, pp. 138–144 Primrose S. B., Wardlaw. S. B. Edited by London:: Academic Press.;
    [Google Scholar]
  9. Le#x00E1;o C., van Uden N. 1986; Transport of lactate and other short-chain monocarboxylates in the yeastCandida utilis. . Appl Microbiol Biotechnol 23:389–393
    [Google Scholar]
  10. Perea J., Gancedo C. 1982; Isolation and characterization of a mutant ofSaccharomyces cerevisiae defective in phosphoenol- pyruvate carboxykinase. Arch Microbiol 132:141–143
    [Google Scholar]
  11. Postma E., Verduyn C, Scheffers .W.A, van Dijken J.P. 1989; Enzymatic analysis of the Crabtree effect in glucose- limited chemostat cultures ofSaccharomyces cerevisiae. . Appl Environ Microbiol 53:468–477
    [Google Scholar]
  12. Sousa M.J., Miranda L, C#x0242;rte-Real M., Le#x00E1;o C. 1996; Transport of acetic acid inZygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acidic environments. Appl Environ Microbiol 62:3152–3157
    [Google Scholar]
  13. van Uden N. 1967; Transport-limited fermentation and growth ofSaccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168
    [Google Scholar]
  14. Witt J., Kronau R., Holzer . 1966; Repression von alkoholdehydrogenase, malatdehydrogenase, isocitratlyase und malatsynthase in hefe durch glucose. Biochim Biophys Acta 118:522–537
    [Google Scholar]
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