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Volume 144, Issue 2

Extracellular enzyme activities potentially involved in the pathogenicity of Mycobacterium tuberculosis Free

Abstract

To evaluate the potential contribution of extracellular enzymes to the pathogenicity of mycobacteria, the presence of selected enzyme activities was investigated in the culture filtrates of the obligate human pathogen BCG, the opportunistic pathogens and , and the non-pathogenic species and . For and , 22 enzyme activities were detected in the culture filtrates and/or cell surfaces, of which eight were absent from the culture fluids of non-pathogens: alanine dehydrogenase, glutamine synthetase, nicotinamidase, isonicotinamidase, superoxide dismutase, catalase, peroxidase and alcohol dehydrogenase. These activities, which correspond to secreted enzymes, formed a significant part (up to 92%) of the total enzyme activities of the bacteria and were absent from the culture fluids and the cell surfaces of the non-pathogenic species and . The extracellular location of superoxide dismutase and glutamine synthetase seemed to be restricted to the obligate pathogens examined. The difference in the enzyme profiles was not attributable to the growth rates of the two groups of bacteria. The presence of the eight enzyme activities in the outermost compartments of obligate pathogens and their absence in those of non-pathogens provides further evidence that these enzymes may be involved in the pathogenicity of mycobacteria. In addition, the eight enzyme activities were demonstrated in the cell extract of . Stepwise erosion of the cell surface of to expose internal capsular constituents showed that the various enzyme activities, with the possible exception of superoxide dismutase, were located more deeply in the cell envelope of this bacterium. This suggests that the molecular architecture of the mycobacterial envelopes may play an important role in the pathogenicity of these organisms.

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Keyword(s): enzyme , extracellular protein , Mycobacterium , pathogenicity and tuberculosis
© Society for General Microbiology 1998
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1998-02-01
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Extracellular enzyme activities potentially involved in the pathogenicity of Mycobacterium tuberculosis
Microbiology 144, 577 (1998); https://doi.org/10.1099/00221287-144-2-577
/content/journal/micro/10.1099/00221287-144-2-577
/content/journal/micro/10.1099/00221287-144-2-577
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