A 996 bp cDNA encoding the ASPND1 immunodominant antigen was cloned and expressed in as a fusion protein with the enzyme glutathione -transferase (GST) from The GST-ASPND1 fusion protein was purified from isolated bacterial inclusion bodies by preparative SDS-PAGE. After cleavage with thrombin, the ASPND1 recombinant antigen (ASPND1r) and the GST protein were separated by SDS-PAGE and immunoblotted with a number of different human sera. The sera from 22 (88%) of 25 patients with an aspergilloma recognized the ASPND1r recombinant antigen on immunoblots. Forty-nine normal human sera and 14 sera from patients with other infections were unreactive. The ASPND1r expressed in could therefore be used, in combination with previously reported recombinant antigens, as a standardized antigen for serological and clinical diagnosis of -associated diseases.


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