1887

Abstract

A genome map of TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with I, I and I. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the chromosome.

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/content/journal/micro/10.1099/00221287-144-2-509
1998-02-01
2020-01-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-2-509
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