1887

Abstract

A genome map of TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with I, I and I. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the chromosome.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-2-509
1998-02-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/2/mic-144-2-509.html?itemId=/content/journal/micro/10.1099/00221287-144-2-509&mimeType=html&fmt=ahah

References

  1. Augustin J., Götz F. 1990; Transformation of Staphylococcus epidermidis and other staphylococcal species with DNA by electroporation.. FEMS Microbiol Lett 66:203–208
    [Google Scholar]
  2. Augustin J., Rosenstein R., Wieland B., Schneider U., Schnell N., Engelke G., Entian K. -D., Götz F. 1992; Genetic analysis of epidermin biosynthetic genes and epidermin-negative mutants of Staphylococcus epidermidis . Eur J Biochem 204:1149–1154
    [Google Scholar]
  3. Ayora S., Götz F. 1994; Genetic and biochemical properties of an extracellular neutral metalloprotease from Staphylococcus hyicus subsp. hyicus . Mol Gen Genet 242:421–430
    [Google Scholar]
  4. Bachmann B. J. 1990; Linkage map of Escherichia coli K12, edition 8.. Microbiol Rev 54:130–197
    [Google Scholar]
  5. Canard B., Cole S. T. 1989; Genome organization of the anaerobic pathogen Clostridium perfringens . Proc Natl Acad Sci USA 86:6676–6680
    [Google Scholar]
  6. Christiansen I., Hengstenberg W. 1996; Cloning and sequencing of two genes from Staphylococcus carnosus coding for glucose specific PTS and their expression in Escherichia coli K- 12.. Mol Gen Genet 250:375–379
    [Google Scholar]
  7. Cole S. T., Saint Girons I. 1994; Bacterial genomics.. FEMS Microbiol Rev 14:139–160
    [Google Scholar]
  8. Demleitner G., Götz F. 1994; Evidence for importance of the Staphylococcus hyicus lipase pro-peptide in lipase secretion, stability, and activity.. FEMS Microbiol Lett 121:189–198
    [Google Scholar]
  9. Dubitsky A., Brown J., Brandwein H. 1992; Chemiluminescent detection of DNA on nylon membranes.. BioTechniques 13:392–399
    [Google Scholar]
  10. Fischer R., Hengstenberg W. 1992; Mannitol-specific enzyme II of the phosphenolpyruvate-dependent phosphotransferase system of Staphylococcus carnosus . Eur J Biochem 204:963–969
    [Google Scholar]
  11. Fonstein M., Haselkorn R. 1995; Physical mapping of bacterial genomes.. J Bacteriol 177:3361–3369
    [Google Scholar]
  12. Götz F. 1986; Ein neues Wirt-Vektor-System bei Staphylococcus carnosus . Umschau 10:530–537
    [Google Scholar]
  13. Götz F. 1990a Development of a cloning system in Staphylococcus carnosus: different processing of the Staphylococcus hyicus lipase in Staphylococcus carnosus and Staphylococcus hyicus . In Molecular Biology of the Staphylococci pp 273–281 Edited by Novick R. P. New York: VCH;
    [Google Scholar]
  14. Götz F. 1990b; Staphylococcus carnosus: a new host organism for gene cloning and protein production.. J Appl Bacteriol Symp Suppl 69:49–53
    [Google Scholar]
  15. Götz F., Schleifer K. H. 1983; Staphylococcus carnosus und seine Verwendung in der Gentechnologie.. Fleischwirtschaft 63:1758–1760
    [Google Scholar]
  16. Götz F., Schumacher B. 1987; Improvements of protoplast transformation in Staphylococcus carnosus . FEMS Microbiol Lett 40:285–288
    [Google Scholar]
  17. Götz F., Kreutz B., Schleifer K. H. 1983; Protoplast transformation of Staphylococcus carnosus by plasmid DNA.. Mol Gen Genet 189:340–342
    [Google Scholar]
  18. Heilmann C., Schweitzer O., Gerke, G, Vanittanakom N., Mack D., Götz F. 1996; Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis . Mol Microbiol 20:1083–1091
    [Google Scholar]
  19. Klein M., Meens J., Freudel R. 1995; Functional characterization of the Staphylococcus carnosus SecA protein in Escherichia coli and Bacillus subtilis secA mutant strains.. FEMS Microbiol Lett 131:271–277
    [Google Scholar]
  20. Kohlbrecher D., Eisermann R., Hengstenberg W. 1992; Staphyloccocal phosphoenolpyruvate-dependent phosphotransferase system: molecular cloning and nucleotide sequence of the Staphylococcus carnosus ptsI gene and expression and complementation studies of the gene product.. J Bacteriol 171:3016–3024
    [Google Scholar]
  21. Kreutz B., Gfitz F. 1984; Construction of Staphylococcus plasmid vector pCA43 conferring resistance to chloramphenicol, arsenate, arsenite and antimony.. Gene 32:301–304
    [Google Scholar]
  22. Lee J. J., Smith H. O., Redfield R. J. 1989; Organization of the Haemophilus influenzae Rd genome.. J Bacteriol 171:3016–3024
    [Google Scholar]
  23. Lerche M., Sinell H. J. 1955; Zum Vorkommen von Kokken in Rohwiirsten.. Arch Lebensmittelhyg 6:194–198
    [Google Scholar]
  24. Liebl W., Götz F. 1986; Lipase directed export of Escherichia coli β-lactamase in Staphylococcus carnosus . Mol Gen Genet 204:166–173
    [Google Scholar]
  25. Liepe H. -U. 1982; Bakterienkulturen und Rohwurst.. Forum Mikrobiol 5:10–15
    [Google Scholar]
  26. Linhardt F., Ziebuhr W., Meyer P., Witte W., Hacker J. 1992; Pulsed-field gel electrophoresis of genomic restriction fragments as a tool for the epidemiological analysis of Staphylococcus aureus and coagulase-negative staphylococci.. FEMS Microbiol Lett 95:181–186
    [Google Scholar]
  27. Meens J., Klose R., Freudel R. 1994; The Staphylococcus carnosus secE gene: cloning, nucleotide sequence and functional characterization in Escherichia coli secE mutant strains.. FEMS Microbiol Lett 117:113–120
    [Google Scholar]
  28. Neubauer H., Götz F. 1996; Physiology and interaction of nitrate and nitrite reduction in Staphylococcus carnosus . J Bacteriol 178:2005–2009
    [Google Scholar]
  29. Niinivaara F. P., Pohja M. S. 1956; liber die Reifung der Rohwurst. I. Mitt: Die Veranderung der Bakterienflora wahrend der Reifung.. Z Lebensm-Unters-Forsch 104:413–422
    [Google Scholar]
  30. Overhoff-Freundlieb B., Freudel R. 1991; Suppression of an Escherichia coli secA ts mutant by a gene cloned from Staphylococcus carnosus . FEMS Microbiol Lett 84:143–150
    [Google Scholar]
  31. Pattee P. A., Lee H., -C. & Bannantine J. P. 1990 Genetic and physical mapping of the chromosome of Staphylococcus aureus . In Molecular Biology of the Staphylococci pp 41–58 Edited by Novick R. P. New York: VCH;
    [Google Scholar]
  32. Piggot P. J. 1990 Genetic map of Bacillus subtilis 168.. In The Bacterial Chromosome pp 107–145 Edited by Drlica K., Rily M. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  33. Prévost G., Jaulhac B., Piemont Y. 1992; DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates.. J Clin Microbiol 30:967–973
    [Google Scholar]
  34. Rosenstein R., Peschel A., Wieland B., Götz F. 1992; Regulation and expression of the Staphylococcus xylosus antimonite, arsenate and arsenite resistance operon.. J Bacteriol 174:3676–3683
    [Google Scholar]
  35. Roussel Y., Pebay M., Guedon G., Simonet J. M., Decaris B. 1994; Physical and genetic map of Streptococcus thermophilus A054.. J Bacteriol 176:7413–7422
    [Google Scholar]
  36. Samuelson P., Hansson M., Ahlborg N., Andréoni C., Götz F., Bäkchi T., Nguyen T. N., Binz H., Uhlén M., Ståhl S. 1995; Cell surface display of recombinant proteins on Staphylococcus carnosus . J Bacteriol 177:1470–1476
    [Google Scholar]
  37. Schleifer K., -H. & Fischer U. 1982; Description of a new species of the genus Staphylococcus: Staphylococcus carnosus . Int J Syst Bacteriol 32:153–156
    [Google Scholar]
  38. Schwartz D. C., Cantor C. R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gel electrophoresis.. Cell 37:67–75
    [Google Scholar]
  39. Snopkovd S., Götz F., Doskar J., Rosypal S. 1994; Pulsed field gel electrophoresis of the genomic restriction fragments of coagulase-negative staphylococci.. FEMS Microbiol Lett 124:131–140
    [Google Scholar]
  40. Strauss A., Götz F. 1996; In vivo immobilization of enzymatically active polypeptides on the cell surface of Staphylococcus carnosus . Mol Microbiol 21:491–500
    [Google Scholar]
  41. Thumm G., Götz F. 1997; Studies on prolysostaphin processing and characterization of the lysostaphin immunity (Lif) of Staphylococcus simulans biovar staphylolyticus . Mol Microbiol 23:1251–1265
    [Google Scholar]
  42. Tschauder S., Driessen A. J. M., Freudel R. 1992; Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family.. Mol Gen Genet 235:147–152
    [Google Scholar]
  43. Tulloch D. L., Finch L. R., Hillier A. J., Davidson B. E. 1991; Physical map of the chromosome of Lacotococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons.. J Bacteriol 173:2768–2775
    [Google Scholar]
  44. Wieland B., Feil C., Gloria-Maercker E., Thumm G., Lechner M., Bravo J.-M., Poralla K., Götz F. 1994; Genetic and biochemical analysis of the biosynthesis of the yellow carotenoid 4,4'- diaponeurosporene of Staphylococcus aureus . J Bacteriol 176:7719–7726
    [Google Scholar]
  45. Wieland K.-P., Wieland B., Gtitz F. 1995; A promoter-screening plasmid and xylose-inducible, glucose-repressible expression vectors for Staphylococcus carnosus . Gene 158:91–96
    [Google Scholar]
  46. Witke C., Götz F. 1993; Cloning, sequencing and characterization of the gene encoding the class I fructose-1,6-bisphos-phate aldolase of Staphylococcus carnosus . J Bacteriol 175:7495–7499
    [Google Scholar]
  47. Witke C., Götz F. 1995; Cloning and nucleotide sequence of the signal peptidase II (lsp)-gene from Staphylococcus carnosus . FEMS Microbiol Lett 126:233–240
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-2-509
Loading
/content/journal/micro/10.1099/00221287-144-2-509
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error