1887

Abstract

is recognized as the major cause of food-borne gastrointestinal disease in the developed world. To facilitate the molecular genetic analysis of this pathogen, an approximately 18-fold redundant Tropist3 cosmid library was constructed from NCTC 11168 genomic DNA. The cosmid library was partially ordered by hybridization to 15 pulsed-field electrophoresis (PFGE) restriction fragments. This analysis confirmed the estimated size of the genome to be 1730 kb, but suggested discrepancies in some regions of the published physical map. The precise locations of two of the three rRNA gene clusters were mapped using a combination of restriction fingerprinting, sample sequencing and riboprobing. Additionally, 15 further genes were located on the rev-recd map. A more detailed physical and genetic map of NCTC 11168 is presented.

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/content/journal/micro/10.1099/00221287-144-2-503
1998-02-01
2021-04-17
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References

  1. Annunziato P. W., Wright L. F., Vann W. F., Silver R. P. 1995; Nucleotide sequence and genetic analysis of the neuB genes in region 2 of the polysialic acid gene cluster of Escherichia coli K1.. J Bacteriol 177:312–319
    [Google Scholar]
  2. Bukanov N. O., Berg D. E. 1994; Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC 11638.. Mol Microbiol 11:509–523
    [Google Scholar]
  3. De Smet K. A. L., Jamil S., Stoker N. G. 1993; Tropist3: a cosmid vector for simplified mapping of both G + C-rich and A + T-rich genomic DNA.. Gene 136:215–219
    [Google Scholar]
  4. Guerry P., Doig P., Alm R. A., Burr D. H., Kinsella N., Trust T. J. 1996; Identification and characterisation of genes required for post-translational modification of Campylobacter coli VC167 flagellin.. Mol Microbiol 19:369–378
    [Google Scholar]
  5. Henderson J., Karlyshev A. V., Wren B. W., Ketley J. M. 1997; Construction of an ordered cosmid library and genetic map of Campylobacter jejuni NCTC 11168. Society for General Microbiology, 136th Ordinary MeetingUniversity of ReadingJanuary 1997 abstract M18
    [Google Scholar]
  6. Jiang Q., Hiratsuka K., Taylor D. E. 1996; Variability of gene order in different Helicobacter pylori strains contributes to genome diversity.. Mol Microbiol 20:833–842
    [Google Scholar]
  7. Karlyshev A. V., Ketley J. M., Wren B. W. 1997; Random shot-gun cloning and sample sequencing of C. jejuni NCTC 11168 genome. Society for General Microbiology, 136th Ordinary MeetingUniversity of ReadingJanaury 1997 abstract 44
    [Google Scholar]
  8. Ketley J. M. 1997; Pathogenesis of enteric infection by Campylobacter . Microbiology 143:5–21
    [Google Scholar]
  9. Kihara M., Homma M., Kutsukake K., Macnab R. M. 1989; Flagella switch of Salmonella typhimurium: gene sequences and deduced protein sequences.. J Bacteriol 171:3247–3257
    [Google Scholar]
  10. Kim N. W., Lombardi R., Bingham H., Hani E., Loule H., Ng D., Chan V. L. 1993; Fine mapping of the three rRNA operons on the updated genomic map of Campylobacter jejuni TGH9011 (ATCC 43431).. J Bacteriol 175:7468–7470
    [Google Scholar]
  11. Lin W. S., Cunneen T., Lee C. Y. 1994; Sequence analysis and molecular characterisation of genes required for the biosynthesis of type 1 capsular polysaccharide in Staphylococcus aureus . J Bacteriol 176:7005–7016
    [Google Scholar]
  12. Moran A. P., Prendergast M. M., Appelmelk B. J. 1996; Molecular mimicry of host structures by bacterial lipopoly-saccharides and its contribution to disease.. FEMS Immunol Med Microbiol 16:105–115
    [Google Scholar]
  13. Newnham E., Chang N., Taylor D. E. 1996; Expanded genomic map of Campylobacter jejuni UA580 and localisation of 23S ribosomal rRNA genes by I-CeuI restriction endonuclease digestion.. FEMS Microbiol Lett 142:223–229
    [Google Scholar]
  14. Taylor D. E., Eaton M., Yan W., Chang N. 1992; Genome maps of Campylobacter jejuni and Campylobacter coli . J Bacteriol 174:2332–2337
    [Google Scholar]
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