Cleavage of chromosomal DNA from PAO by I and I has been used together with PFGE and Southern hybridization to establish the map location of the following principal denitrification genes: (encoding the large and small subunits of respiratory nitrate reductase), (cytochrome- nitrite reductase), (uroporphyrinogen-III methyltransferase for haem biosynthesis), (nitric-oxide reductase complex), (nitrous-oxide reductase) and (an outer-membrane protein and OprC homologue). The study also included several genes related to anaerobic or microaerophilic metabolism: (encoding the catalytic subunit of the periplasmic nitrate reductase), (catalytic subunit of the cytochrome- oxidase), (oxygen-independent coproporphyrinogen-III oxidase), an -like regulatory gene, and and (electron carriers azurin and ferredoxin, respectively). Genes necessary for denitrification are concentrated at 20 to 36 min on the chromosome, where they form three separate loci, the and gene clusters. Genomic DNA of ZoBell was also subjected to I restriction and Southern analysis to assign denitrification genes to individual fragments. A homologue of encoding a putative component of the Cu-processing apparatus for nitrous-oxide reductase was identified. In both and there is evidence for the linkage of () with and ; and for the presence of a gene.


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