1887

Abstract

A 28 kDa protein associated with the inner membrane was induced strongly in K-12 cells grown in the presence of a hydrophobic organic solvent, n-hexane or cyclooctane. These organic solvents suppressed the growth (growth rate and yield) of . A partial amino acid sequence showed that this protein was the phage-shock protein PspA. PspA is known to be induced in cells under extreme stress conditions. The results suggest that cells are subject to strong stress in the presence of organic solvents. Introduction of a multi-copy plasmid vector carrying the operon into improved the survival frequency of cells exposed suddenly to n-hexane but not the growth rate of cells growing in the presence of n-hexane.

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1998-02-01
2021-07-29
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References

  1. Aono R., Aibe K., Inoue A., Horikoshi K. 1991; Preparation of organic solvent-tolerant mutants from Escherichia coli K-12.. Agric Biol Chem 55:1935–1938
    [Google Scholar]
  2. Aono R., Doukyu N., Kobayashi H., Nakajima H., Horikoshi K. 1994a; Oxidative bioconversion of cholesterol by Pseudomonas sp. strain ST-200 in a water-organic solvent two phase system.. Appl Environ Microbiol 60:2518–2523
    [Google Scholar]
  3. Aono R., Kobayashi H., Joblin K., Horikoshi K. 1994b; Effects of organic solvents on growth of Escherichia coli K-12.. Biosci Biotech Biochem 58:2009–2014
    [Google Scholar]
  4. Aono R., Negishi T., Aibe K., Inoue A., Horikoshi K. 1994c; Mapping of organic solvent tolerance gene ostA in Escherichia coli K-12.. Biosci Biotechnol Biochem 58:1231–1235
    [Google Scholar]
  5. Aono R., Negishi T., Nakajima H. 1994d; Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli . Appl Environ Microbiol 60:4624–4626
    [Google Scholar]
  6. Asako H., Nakajima H., Kobayashi K., Kobayashi M., Aono R. 1997; Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli . Appl Environ Microbiol 63:1428–1433
    [Google Scholar]
  7. Bergler H., Abraham D., Aschauer H., Turnowsky F. 1994; Inhibition of lipid biosynthesis induces the expression of the psp A gene.. Microbiology 140:1937–1944
    [Google Scholar]
  8. Brissette J. L., Russel M., Weiner L., Model P. 1990; Phage shock protein, a stress protein of Escherichia coli . Proc Natl Acad Sci USA 87:862–867
    [Google Scholar]
  9. Brissette J., L, Weiner L., Ripmaster T. L., Model P. 1991; Characterization and sequence of the Escherichia coli stress-induced psp operon.. J Mol Biol 220:35–48
    [Google Scholar]
  10. Camille F., Fletcher G., Wulft J. L., Earhart C. F. 1973; Solubilization of cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-laurylsarcosinate.. J Bacterial 115:717–722
    [Google Scholar]
  11. Doukyu N., Aono R. 1997; Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200.. Extremophiles 1:100–105
    [Google Scholar]
  12. Favre–Bulle O., Schouten T., Kingma J., Witholt B. 1991; Bioconversion of n-octane to octanic acid by a recombinant Escherichia coli cultured in a two-liquid phase bioreactor.. Bio/Technology 9:367–371
    [Google Scholar]
  13. Harbron S., Smith B. W., Lilly M. D. 1986; Two-liquid phase biocatalysis: epoxidation of 1,7-octadiene by Pseudomonas putida . Enzyme Microb Technol 8:85–88
    [Google Scholar]
  14. Inoue A., Horikoshi K. 1989; A Pseudomonas thrives in high concentrations of toluene.. Nature 338:264–265
    [Google Scholar]
  15. Kingsman A. J., Clarke L., Mortimer R. K., Carbon J. 1979; Replication in Saccharomyces cerevisiae of plasmid pBR31 3 carrying DNA from the yeast trpl region.. Gene 7:141–152
    [Google Scholar]
  16. Kleerebezem M., Tommassen J. 1993; Expression of the psp Agene stimulates efficient protein export in Escherichia coli . Mol Microbiol 7:957–965
    [Google Scholar]
  17. Kleerebezem M., Crielaard W., Tommassen J. 1996; Involvement of stress protein PspA (phage shock protein A) of Escherichia coli in maintenance of the protonmotive force under stress conditions.. EMBO J 15:162–171
    [Google Scholar]
  18. Kohara Y., Akiyama K., Isono K. 1987; The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.. Cell 50:495–508
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  20. Leo A. J. 1993; Calculating log P oct from structures.. Chem Rev 93:1281–1306
    [Google Scholar]
  21. Luria S. E., Burrous J. W. 1957; Hybridization between Escherichia coli and Shigella . J Bacteriol 74:461–476
    [Google Scholar]
  22. Model P., Jovanovic G., Dworkin J. 1997; The Escherichia coli phage-shock-protein (psp) operon.. Mol Microbiol 24:255–261
    [Google Scholar]
  23. Murray N. E., Brammer W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants.. Mol Gen Genet 150:53–61
    [Google Scholar]
  24. Nakajima H., Kobayashi K., Kobayashi M., Asako H., Aono R. 1995a; Overexpression of the rob A gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli . Appl Environ Microbiol 61:2302–2307
    [Google Scholar]
  25. Nakajima H., Kobayashi M., Negishi T., Aono R. 1995b; soxRS gene increased the level of organic tolerance in Escherichia coli . Biosci Biotechnol Biochem 59:1323–1325
    [Google Scholar]
  26. Noguchi K., Nakajima H., Aono R. 1997; Effects of oxygen and nitrate on growth of Escherichia coli and Pseudomonas aeruginosa in the presence of organic solvents.. Extremophiles 1:193–198
    [Google Scholar]
  27. Sikkema J., de Bont J. A. M., Poolman B. 1994; Interactions of cyclic hydrocarbons with biological membranes.. J Biol Chem 269:8022–8028
    [Google Scholar]
  28. de Smet M.-J., Kingma J., Wynberg H., Witholt B. 1983; Pseudomonas oleovorans as a tool in bioconversions of hydrocarbons: growth, morphology and conversion characteristics in different two-phase systems.. Enzyme Microb Technol 5:352–360
    [Google Scholar]
  29. Van Sonsbeck H. M., Beeftink H. H., Tramper T. 1993; Two-liquid-phase bioreactors.. Enzyme Microb Technol 15:722–729
    [Google Scholar]
  30. Weiner L., Model P. 1994; Role of Escherichia coli stress-response operon in stationary-phase survival.. Proc Natl Acad Sci USA 91:2191–2195
    [Google Scholar]
  31. Weiner L., Brissette J. L., Model P. 1991; Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on σ54 and modulated by positive and negative feedback mechanisms.. Genes Dev 5:1912–1923
    [Google Scholar]
  32. Yanisch-Perron C., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
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