Characterization and possible functions of a new filamentous bacteriophage from Vibrio cholerae 0139 Free

Abstract

The emergence and rapid rise to dominance of O139 in India and Bangladesh in 1992 led to the consideration that choleraphage might serve as both a selective mechanism and a means for horizontal transmission of genetic information. A filamentous phage ‘493′ from O139 strain AJ27-493 has been purified and partially characterized. The phage was inactive on classical biotype 01 but it was active on El Tor biotype strains isolated prior to 1994 when El Tor re-emerged in Bangladesh. More recent El Tor isolates were all resistant to the phage. The phage was also active on O139 strains. Unlike the filamentous ctxφ, the receptor for 493 is not TcpA. The phage genome was a 9.3 kb closed circular single-stranded molecule containing a 0.4 kb double-stranded stem supporting a 2 kb single-stranded loop. A 283 bp fragment was cloned and used as a probe in Southern hybridization, in parallel with total phage 493 DNA. These probes hybridized both chromosomally and extrachromosomally with most O139 strains, but not with O1 strains. Infection of hybridization-negative El Tor or O139 strains resulted in the presence of hybridizing loci (both plasmid and chromosomal), in the appearance of an 18 kDa protein, and in marked alterations in colonial morphology. Phage 493 is clearly distinct from other O139 choleraphages which have been described. Phage 493 DNA hybridized with an encapsulated non-O1 (O31) strain (NRT36S) which was isolated before O139 was recognized. NRT36S also produces a phage which can infect El Tor strains with low efficiency. Further studies may reveal whether bacteriophage play a role in the emergence and the territoriality of new choleragenic vibrios.

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1998-02-01
2024-03-28
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References

  1. Albert M. J. 1994; Vibrio cholerae O139 Bengal.. J Clin Microbiol 32:2345–2349
    [Google Scholar]
  2. Anonymous 1883; Dr. Koch’s newly described cholera-organism.. Br Med J 1883:828–829
    [Google Scholar]
  3. Bik E. M., Bunschoten A. E., Gouw R. D., Mooi F. R. 1995; Genesis of the novel epidemic Vibrio cholerae O139 strain: evidence for horizontal transfer of genes involved in polysaccharide synthesis.. EMBO J 14:209–216
    [Google Scholar]
  4. Faruque A. S. G., Fuchs G. J., Albert M. J. 1996; Changing epidemiology of cholera due to Vibrio cholerae O1 and O139 Bengal in Dhaka, Bangladesh.. Epidemiol Infect 116:275–278
    [Google Scholar]
  5. Finkelstein R. A., Boesman-Finkelstein M., Sengupta D. K., Page W. J., Phillips T. E. 1997; Colonial opacity variations among the choleragenic vibrios.. Microbiology 143:23–34
    [Google Scholar]
  6. Fraenkel-Conrat H. 1985 The Viruses: Catalogue, Characterization, and Classification New York: Plenum;
    [Google Scholar]
  7. Garon C. F. 1982 Electron microscopy of nucleic acids.. In Gene Amplification and Analysis vol 2 pp 573–585 Edited by Chirikjian J. G., Papas T. S. New York: Elsevier;
    [Google Scholar]
  8. Garon C. F. 1986 Electron microscopy of nucleic acids.. In Ultrastructure Techniques for Microorganisms pp 161–181 Edited by Aldrich H. C., Todd W. J. New York: Plenum;
    [Google Scholar]
  9. Gay F. P. 1935 The vibrios and Asiatic cholera.. In Agents of Disease and Host Resistance pp 597–598 Springfield, IL & Baltimore, MD: C. C. Thomas;
    [Google Scholar]
  10. Guidolin A., Manning P. A. 1987; Genetics of Vibrio cholerae and its bacteriophages.. Microbiol Rev 51:285–298
    [Google Scholar]
  11. Hacker J., Blum-Oehler G., Muhldorfer I., Tschäpe H. 1997; MicroReview: pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution.. Mol Microbiol 23:1089–1097
    [Google Scholar]
  12. International Centre for Diarrhoeal Disease Research, Bangladesh 1996; Research highlights of 1995.. Glimpse 18:3
    [Google Scholar]
  13. Isshiki Y., Kondo S., Iguchi T., Sano Y., Shimoda T., Hisatsune K. 1996; An immunochemical study of serological cross-reaction between lipopolysaccharides from Vibrio cholerae O22 and O139.. Microbiology 142:1499–1504
    [Google Scholar]
  14. Johnson J. A., Panigrahi P., Morris J. G. Jr 1992; Non-O1 Vibrio cholerae NRT36S produces a polysaccharide capsule that determines colony morphology, serum resistance, and virulence in mice.. Infect Immun 60:864–869
    [Google Scholar]
  15. Kaper J. B., Morris J. G. Jr, Levine M. M. 1995; Cholera.. Clin Microbiol Rev 8:48–86
    [Google Scholar]
  16. Kar S., Ghosh R. K., Ghosh A. N., Ghosh A. 1996; Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae O139 into the host chromosomal DNA.. FEMS Microbiol Lett 145:17–22
    [Google Scholar]
  17. Knirel Y. A., Senchenkova S. N., Jansson P. -E., Weintraub A., Ansaruzzaman M., Albert M. J. 1996; Structure of the O-specific polysaccharide of an Aeromonas trota strain cross-reactive with Vibrio cholerae O139 Bengal.. Eur J Biochem 238:160–165
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  19. Litwin C. M., Boyko S. A., Calderwood S. B. 1992; Cloning, sequencing, and transcriptional regulation of the Vibrio cholerae fur gene.. J Bacteriol 174:1897–1903
    [Google Scholar]
  20. Miller V. L., Mekalanos J. J. 1988; A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR . J Bacteriol 170:2575–2583
    [Google Scholar]
  21. Mitra S. N. 1989; Mutation induced by vibriophage PS166 infection changes biotype and phage type of Vibrio cholerae . J Med Microbiol 30:137–141
    [Google Scholar]
  22. Mooi F. R., Bik E. M. 1997; The evolution of epidemic Vibrio cholerae strains.. Trends Microbiol 5:161–165
    [Google Scholar]
  23. de Moor C. E. 1939; Epidemic cholera in South Celebes caused by vibrio El Tor.. Meded Drenst Volksgezondheid Ned-Indië 28:320–355
    [Google Scholar]
  24. Mukerjee S. 1974 Vibrio phages and phage-typing of cholera vibrios.. In Cholera pp 61–74 Edited by Barua D., Burrows W. Philadelphia, PA: W. B. Saunders;
    [Google Scholar]
  25. Pajni S., Chowdhury N. R., Ghosh A., Kar S., Ghosh R. K. 1995a; Characterization of phage O139, a Vibrio cholerae O139 temperate bacteriophage with cohesive DNA termini.. FEMS Microbiol Lett 131:69–74
    [Google Scholar]
  26. Pajni S., Sharma C., Bhasin N., Ghosh A., Ramamurthy T., Nair G. B., Ramajayami S., Das B., Kar S., Roychowdury S., Ghosh R. K. 1995b; Studies on the genesis of Vibrio cholerae O139: identification of probable progenitor strains.. J Med Microbiol 42:20–25
    [Google Scholar]
  27. Parker C., Richardson S. H., Romig W. R. 1970; Production of bacteriophage-associated materials by Vibrio cholerae: possible correlation with pathogenicity.. Infect Immun 1:417–420
    [Google Scholar]
  28. Rasched I., Oberer E. 1986; Ff coliphages: structural and functional relationships.. Microbiol Rev 50:401–427
    [Google Scholar]
  29. Reidl J., Mekalanos J. J. 1995; Characterization of Vibrio cholerae bacteriophage K139 and use of a novel mini-transposon to identify a phage-encoded virulence factor.. Mol Microbiol 18:685–701
    [Google Scholar]
  30. Rowe B., Frost J. A. 1992 Vibrio phages and phage-typing.. In Cholera pp 95–105 Edited by Barua D., Greenough W. B. New York: Plenum;
    [Google Scholar]
  31. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Sengupta D. K., Boesman-Finkelstein M., Finkelstein R. A. 1996; Antibody against the capsule of Vibrio cholerae O139 protects against experimental challenge.. Infect Immun 64:343–345
    [Google Scholar]
  33. Sharma C., Nair G. B., Mukhopadhyay A. K., Bhattacharya S. K., Ghosh R. K., Ghosh A. 1997; Molecular characterization of Vibrio cholerae O1 biotype El Tor strains isolated between 1992 and 1995 in Calcutta, India: evidence for the emergence of a new clone of the El Tor biotype.. J Infect Dis 175:1134–1141
    [Google Scholar]
  34. Siddiqui A. I., Bhattacharyya F. K. 1987; Phage-induced change of toxigenesis in Vibrio cholerae . J Med Microbiol 23:331–334
    [Google Scholar]
  35. Stroeher U. H., Jedani K. E., Dredge B. K., Morona R., Brown M. H., Karageorgos L. E., Albert M. J., Manning P. A. 1995; Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139.. Proc Natl Acad Sci USA 92:10374–10378
    [Google Scholar]
  36. Takeya K., Shimodori S. 1963; ‘Prophage-typing’ of El Tor vibrios.. J Bacteriol 65:957–958
    [Google Scholar]
  37. Thelin K. H., Taylor R. K. 1996; Toxin-coregulated pilus, but not mannose-sensitive hemagglutinin is required for colonization by Vibrio cholerae O1 El Tor biotype and O139 strains.. Infect Immun 64:2853–2856
    [Google Scholar]
  38. Waldor M. K., Mekalanos J. J. 1994; ToxR regulates virulence gene expression in non-O1 strains of Vibrio cholerae that cause epidemic cholera.. Infect Immun 62:72–78
    [Google Scholar]
  39. Waldor M. K., Mekalanos J. J. 1996; Lysogenic conversion by filamentous phage encoding cholera toxin.. Science 272:1910–1914
    [Google Scholar]
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