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Volume 144, Issue 2

Population structure and diversity of avian isolates of Pasteurella multocida from Australia Free

Abstract

A total of 110 isolates of from Australian poultry and reference strains for the 16 somatic serovars plus the three subspecies () were analysed to examine their population structure and diversity. The 81 field isolates examined by multilocus enzyme electrophoresis (MLEE) were diverse, being divided into 56 electrophoretic types (ETs), with the 19 reference strains in another 15 ETs. The population was clonal and somatic serotyping was not particularly useful in establishing relationships between isolates. The 71 ETs formed three distinct subclusters (A, B and C) at a genetic distance of 0.36. Biovars tended to be associated with these subclusters: A with biovars 1, 3, 4, 5 and 8 and B with biovars 2, 6, 7, 9 and 10. Ribotyping, performed on all 110 isolates using ll, recognized 21 ribotypes forming nine clusters (R1-R9). The isolates in ribotype cluster R1 were almost identical to those in MLEE cluster B. Using both MLEE and ribotyping, the 19 non-Australian reference strains were found to be distributed over the full diversity of the Australian isolates of . This study has shown that a range of clones are associated with fowl cholera in Australia and that many of the Australian isolates are similar to non-Australian reference strains. Both the MLEE results and the ribotyping data identified a previously unrecognized subset of strains.

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Keyword(s): genetic diversity , multilocus enzyme electrophoresis , Pasteurella multocida and ribotype
© Society for General Microbiology 1998
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1998-02-01
2024-04-20
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References

  1. Blackall P. J., Pahoff J. L., Marks D., Fegan N., Morrow C. J. 1995; Characterisation of Pasteurella multocida isolates from fowl cholera outbreaks on seven turkey farms.. Aust Vet J 72:135–138
     [Google Scholar]
  2. Carpenter T. E., Snipes K. P., Kasten R. W., Hird D. W., Hirsh D. C. 1991; Molecular epidemiology of Pasteurella multocida in turkeys.. Am J Vet Res 52:1345–1349
     [Google Scholar]
  3. Christiansen K. H., Carpenter T. E., Snipes K. P., Hird D. W. 1992a; Transmission of Pasteurella multocida on Californian turkey premises in 1988–89.. Avian Dis 36:262–271
     [Google Scholar]
  4. Christiansen K. H., Carpenter T. E., Snipes K. P., Hird D. W., Ghazikhanian Y. 1992b; Restriction endonuclease analysis of Pasteurella multocida isolates from three California turkey premises.. Avian Dis 36:272–281
     [Google Scholar]
  5. Fegan N., Blackall P. J., Pahoff J. L. 1995; Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry.. Vet Microbiol 47:281–286
     [Google Scholar]
  6. Feizabadi M. M., Robertson I. D., Cousins D. V., Dawson D., Chew W., Gilbert G. L., Hampson D. J. 1996; Genetic characterization of Mycobacterium avium isolates recovered from humans and animals in Australia.. Epidemiol Infect 116:41–49
     [Google Scholar]
  7. Hampson D. J., Blackall P. J., Woodward J. M., Lymbery A. J. 1993a; Genetic analysis of Actinobacillus pleuropneumoniae and comparison with Haemophilus Taxon ‘minor group’ and Taxon C . Zentralbl Bakteriol 279:83–91
     [Google Scholar]
  8. Hampson D. J., Trott D. J., Clarke I. L., Mwaniki C. G., Robertson I. D. 1993b; Population structure of Australian isolates of Streptococcus suis . J Clin Microbiol 31:2895–2900
     [Google Scholar]
  9. Heddleston K. L., Gallagher J. E., Rebers P. A. 1972; Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species.. Avian Dis 16:925–936
     [Google Scholar]
  10. Hirsh D. C., Jessup D. A., Snipes K. P., Carpenter T. E., Hird D. W., McCapes R. C. 1990; Characteristics of Pasteurella multocida isolated from waterfowl and associated avian species in California.. J Wildl Dis 26:204–209
     [Google Scholar]
  11. Kim C. J., Nagaraja K. V. 1990; DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys.. Am J Vet Res 51:207–210
     [Google Scholar]
  12. Lane D. J. 1991 16S/23S rRNA sequencing.. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  13. Lee J. I., Hampson D. J., Combs B. G., Lymbery A. J. 1993; Genetic relationships between isolates of Serpulina (Treponema) byodysenteriae, and comparison of methods for their subspecific differentiation.. Vet Microbiol 34:35–46
     [Google Scholar]
  14. Maynard Smith J., Smith N. H., O'Rourke M., Spratt B. G. 1993; How clonal are bacteria?. Proc Natl Acad Sci USA 90:4384–4388
     [Google Scholar]
  15. Musser J. M. 1996; Molecular population genetic analysis of emerged bacterial pathogens: selected insights.. Emerg Infect Dis 2:1–17
     [Google Scholar]
  16. Mutters R., Ihm P., Pohl S., Frederiksen W., Mannheim W. 1985; Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa . Int J Syst Bacteriol 35:309–322
     [Google Scholar]
  17. Nei M. 1978; Estimation of average heterozygosity and genetic distance from a small number of individuals.. Gene 89:583–590
     [Google Scholar]
  18. Ng L. K., Dillon J. R. 1993; Typing by serovar, antibiogram, plasmid content, riboprobing, and isoenzyme typing to determine whether Neisseria gonorrhoeae isolates requiring proline, citrul- line, and uracil for growth are clonal.. J Clin Microbiol 31:1555–1561
     [Google Scholar]
  19. O'Farrell P. H., Kutter E., Nakanishi M. 1980; A restriction map of the bacteriophage T4 genome.. Mol Gen Genet 179:421–435
     [Google Scholar]
  20. Quentin R., Huet H., Wang F. -S., Geslin P., Goudeau A., Selander R. K. 1995; Characterization of Streptococcus aga lactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal disease.. J Clin Microbiol 33:2576–2581
     [Google Scholar]
  21. Rhoades K. R., Rimler R. B. 1987; Capsular groups of Pasteurella multocida isolated from avian hosts.. Avian Dis 31:895–898
     [Google Scholar]
  22. Rimler R. B., Glisson J. R. 1997 Fowl cholera.. In Diseases of Poultry pp 143–159 Edited by Calnek B. W., Barnes H. J., Beard C. W., McDougald L. R., Saif Y. M. Ames: Iowa State University Press;
     [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  24. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.. Appl Environ Microbiol 51:873–884
     [Google Scholar]
  25. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: W. H. Freeman & Co;
     [Google Scholar]
  26. Snipes K. P., Hirsh D., C, Kasten R. W., Carpenter T. E., Hird D. W., McCapes R. H. 1990; Differentiation of field isolates of Pasteurella multocida serotype 3,4 from live vaccine strain by genotypic characterization.. Avian Dis 34:419–424
     [Google Scholar]
  27. Tomayko J. F., Murray B. E. 1995; Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis.. J Clin Microbiol 33:2903–2907
     [Google Scholar]
  28. Weis N., Lind I. 1996; Usefulness of the DNA-fingerprinting pattern and the multilocus enzyme electrophoresis profile in the assessment of outbreaks of meningococcal disease.. Epidemiol Infect 116:103–114
     [Google Scholar]
  29. White D. G., Dho-Moulin M., Wilson R. A., Whittam T. S. 1993a; Clonal relationships and variation in virulence among Escherichia coli strains of avian origin.. Microb Pathog 14:399–409
     [Google Scholar]
  30. White D. G., Wilson R. A., Emery D. A., Nagaraja K. V., Whittam T. S. 1993b; Clonal diversity among strains of Escherichia coli incriminated in turkey colisepticemia.. Vet Microbiol 34:19–34
     [Google Scholar]
  31. Whittam T. S., Wilson R. A. 1988; Genetic relationships among pathogenic strains of avian Escherichia coli . Infect Immun 56:2458–2466
     [Google Scholar]
  32. Wilson M. A., Morgan M. J., Barger G. E. 1993; Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates.. J Clin Microbiol 31:255–259
     [Google Scholar]
  33. Wilson M. A., Duncan R. M., Nordholm G. E., Berlowkski B. M. 1995; Pasteurella multocida isolated from wild birds of North America: a serotype and DNA fingerprint study of isolates from 1978 to 1993.. Avian Dis 39:587–593
     [Google Scholar]
  34. Woods T. C., Helsel L. O., Swaminathan B., Bibb W. F., Pinner R. W., Gellin B. G., Collin S. F., Waterman S. H., Reeves M. W., Brenner D. J., Broome C. V. 1992; Characterization of Neisseria meningitidis serogroup C by multilocus enzyme electrophoresis and ribosomal DNA restriction endonuclease profiles (ribotyping).. J Clin Microbiol 30:132–137
     [Google Scholar]
  35. Woodward J. M., Connaughton I. D., Fahy V. A., Lymbery A. J., Hampson D. J. 1993; Clonal analysis of Escherichia coli of serogroups 09, 020, and O10l isolated from Australian pigs with neonatal diarrhea.. J Clin Microbiol 31:1185–1188
     [Google Scholar]
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Population structure and diversity of avian isolates of Pasteurella multocida from Australia
Microbiology 144, 279 (1998); https://doi.org/10.1099/00221287-144-2-279
/content/journal/micro/10.1099/00221287-144-2-279
/content/journal/micro/10.1099/00221287-144-2-279
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