1887

Abstract

Summary: Fission yeast cells grow by extension at the ends (poles) and divide by transverse fission. It has previously been reported that var. can switch to unipolar, filamentous growth. Here it is shown that the yeast-to-mycelium transition is a gradual process involving a changeover to unipolar growth associated with asymmetric divisions, the development of large polarly located vacuoles, the modifications of the actin and microtubular cytoskeleton and the repression of cell separation after division. High concentrations of glucose in the medium or supplementation of the medium with caffeine or cAMP support the bipolar yeast phase, inhibit the transition to the mycelial phase and induce the conversion of hyphae to yeasts. These effects suggest that cAMP may be involved in the regulation of dimorphism. Temperatures below 18 °C or over 35 °C are restrictive for the mycelial phase and provoke a return to yeast phase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-12-3475
1998-12-01
2021-04-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/12/mic-144-12-3475.html?itemId=/content/journal/micro/10.1099/00221287-144-12-3475&mimeType=html&fmt=ahah

References

  1. Alfa C., Fantes P., Hyams J., McLeod M., Warbrick E. 1993 Experiments with Fission Yeasts. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Allan V. 1995; Membrane traffic motors.. FEBS Lett 369:101–106
    [Google Scholar]
  3. Beach D., Rodgers L., Gould J. 1985; RAN1+ controls the transition from mitotic division to meiosis in fission yeast.. Curr Genet 10:297–311
    [Google Scholar]
  4. Benko Z., Sipiczki M. 1992; Caffeine tolerance in Schizo- saccharomyces pombe: physiological adaptation and interaction with theophylline.. Can J Microbiol 39:551–554
    [Google Scholar]
  5. Byrne S.M., Hoffman C.S. 1993; Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe.. J Cell Sci 105:1095–1100
    [Google Scholar]
  6. Gimeno C.J., Ljungdahl P.O., Style GA., Fink G.R. 1992; Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS.. Cell 68:1077–1090
    [Google Scholar]
  7. Gold S., Duncan G., Barrett K., Kronstad J. 1994; cAMP regulates morphogenesis in the fungal pathogen Ustilago maydis.. Genes Dev 8:2805–2816
    [Google Scholar]
  8. Gow N.A.R. 1994; Growth and guidance of the fungal hypha.. Microbiology 140:3193–3205
    [Google Scholar]
  9. Grallert A., Miklos 1., Sipiczki M. 1997; Division site selection, cell separation and formation of anucleate minicells in Schizo-saccharomyces pombe mutants resistant to cell wall lytic enzymes.. Protoplasma 198:218–229
    [Google Scholar]
  10. Hagan I.M., Hyams J.S. 1988; The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.. J Cell Sci 89:343–357
    [Google Scholar]
  11. Johnson B.F., Calleja G.B., Boisdair I., Yoo B.Y. 1979; Cell division in yeasts. III. The biased, asymmetric location of the septum in the fission yeast cell, Schizosaccharomyces pombe.. Exp Cell Res 123:253–259
    [Google Scholar]
  12. Kron S.J., Styles C.A., Fink G.R. 1994; Symmetric cell division in pseudohyphae of the yeast Saccharomyces cerevisiae.. Mol Biol Cell 5:1003–1022
    [Google Scholar]
  13. Larsen A., Sypherd P. 1974; Cyclic adenosine 3,,5/-mono-phosphate and morphogenesis in Mucor racemosus.. J Bacteriol 117:432–438
    [Google Scholar]
  14. McDonald I.J., Tsai C.S. 1989; Continuous culture and intermediary carbon metabolism.. In Molecular Biology of the Fission Yeast pp. 367–396 Edited by Nasim A., Young P., Johnson B. F. San Diego: Academic Press;
    [Google Scholar]
  15. Madhani H.D., Fink G.R. 1998; The riddle of MAP kinase signalling specificity.. Trends Genet 14:151–155
    [Google Scholar]
  16. Maeda T., Mochizuki N., Yamamoto M. 1990; Adenylyl cyclase is dispensable for vegetative cell growth in the fission yeast Schizosaccharomyces pombe.. Proc Natl Acad Sci USA 877814–7818
    [Google Scholar]
  17. Maresca B., Medoff G., Schlessinger D., Kobayashi G.S. 1977; Regulation of dimorphism in the pathogenic fungus Histoplasma capsulatum.. Nature 266:447–448
    [Google Scholar]
  18. Marks J., Hyams J.S. 1985; Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe.. Eur J Cell Biol 39:27–32
    [Google Scholar]
  19. Mitchison J.M., Nurse P. 1985; Growth in cell length in the fission yeast Schizosaccharomyces pombe.. J Cell Sci 75:357–376
    [Google Scholar]
  20. Miyata H., Miyata M., Johnson B.F. 1986; Patterns of extension growth of the fission yeast, Schizosaccharomyces pombe.. Can J Microbiol 32:528–530
    [Google Scholar]
  21. Orlowski M., Ross J.F. 1981; Relationship of internal cyclic AMP levels, rates of protein synthesis and Mucor dimorphism.. Arch Microbiol 129:353–356
    [Google Scholar]
  22. Pancaldi S., Dall´Olio G., Poli F., Fasulo M.P., Vannini G.L. 1989; Morphological changes induced by aniline blue in Schizosaccharomyces pombe.. Microbios 57:27–31
    [Google Scholar]
  23. Roberts R.L., Fink G.R. 1995; Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.. Genes Dev 8:2974–2985
    [Google Scholar]
  24. Sabie F.T., Gadd G.M. 1992; Effect of nucleosides and nucleotides and the relationship between cellular adenosine 3′ :5′- cyclic monophosphate (cyclic AMP) and germ tube formation in Candida albicans.. Mycopathologia 119:147–156
    [Google Scholar]
  25. Sipiczki M., Ferenczy L. 1977; Protoplast fusion of Schizo-saccharomyces pombe auxotrophic mutants of identical mating- type.. Mol Gen Genet 151:77–81
    [Google Scholar]
  26. Sipiczki M., Takeo K. 1998; The effect of caffeine on cell cycle progression and polar growth in Schizosaccharomyces pombe.. Biologia (Bratisl) 53:295–300
    [Google Scholar]
  27. Sipiczki M., Grallert B., Miklos I. 1993; Mycelial and syncytial growth in Schizosaccharomyces pombe induced by novel septation mutations.. J Cell Sci 104:485–493
    [Google Scholar]
  28. Sipiczki M., Takeo K., Yamaguchi M., Yoshida S., Miklos I. 1998; Environmentally controlled dimorphic cycle in a fission yeast.. Microbiology 144:1319–1330
    [Google Scholar]
  29. Svoboda A., Slaninová I. 1997; Colocalization of microtubules and mitochondria in the yeast Schizosaccharomyces japonicus var.versatilis.. Can J Microbiol 43:945–953
    [Google Scholar]
  30. Terasaki M., Reese T.S. 1994; Interactions among endo-plasmic reticulum, microtubules, and retrograde movements of the cell surface.. Cell Motil Cytoskelet 29:291–300
    [Google Scholar]
  31. Yarrow D. 1984; Genus 22.Saccharomyces Meyen ex Rees.. In The Yeasts. A Taxonomic Study pp. 379–395 Edited by Kreger-van Rij N. J. W. Amsterdam: Elsevier;
    [Google Scholar]
  32. Zaitsevskaya-Carter T., Cooper J.A. 1997; Spml, a stress-activated MAP kinase that regulates morphogenesis in S.pombe. EMBO J 16:1318–1331
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-12-3475
Loading
/content/journal/micro/10.1099/00221287-144-12-3475
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error