1887

Abstract

Summary: Uptake of iron from various siderophores by a Δfet3Δfet4 strain of was investigated. The catecholate enterobactin and the hydroxamate coprogen were taken up by the cells by passive diffusion, whereas the hydroxamates ferrioxamine B (FOB) and ferricrocin (FC) were taken up via a high-affinity energy-dependent mechanism. The kinetics of FOB and FC uptake showed reciprocal competitive inhibition. The transport was regulated by iron availability, but was independent of the Aft1p and Mac1p transcriptional activators. Mutants affected in the transport of FOB were isolated. The transport of FC was not impaired in these mutants. Functional complementation of one mutant allowed the identification of the gene (Siderophore iron Transport) encoding a putative permease belonging to the major facilitator superfamily. The Sit1 protein is probably a permease specific for the transport of ferrioxamine-type siderophores. The evidence suggests that the uptake of ferrichrome-type siderophores like FC involves other specific permease(s), although there seems to be a common handling of FOB and FC following their internalization by the cell.

Keyword(s): iron uptake , siderophore and yeast
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/content/journal/micro/10.1099/00221287-144-12-3455
1998-12-01
2021-05-09
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References

  1. Askwith C., Kaplan J. 1998; Iron and copper transport in yeast and its relevance to human disease.. Trends Biochem Sci 23:135–138
    [Google Scholar]
  2. Askwith C, Eide D., VanHo A., Bernard P.S., Li L, Davis-Kaplan S., Sipe D.M., Kaplan J. 1994; The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake.. Cell 76:403–110
    [Google Scholar]
  3. Crowley D.E., Reid C.P.P., Szaniszlo P.J. 1987; Microbial siderophores as iron sources for plants.. In Iron Transport in Microbes, Plants and Animals pp. 375–386 Winkelmann G., van der Helm D., Neilands J.B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
  4. Dancis A., Klausner R.D., Hinnebusch A.G., Barriocanal J.G. 1990; Genetic evidence that ferric reductase is required for iron uptake in Saccbaromyces cerevisiae. . Mol Cell Biol 10:2294–2301
    [Google Scholar]
  5. Dix D.R., Bridgham J.T., Broderius M.A., Byersdorfer C.A., Eide D.J. 1994; The FET4 gene encodes the low affinity Fe(II) transport protein of Saccbaromyces cerevisiae. . J Biol Chem 269:26092–26099
    [Google Scholar]
  6. Dix D., Bridgham J., Broderius M., Eide D. 1997; Characterization of the FET4 protein of yeast. Evidence for a direct role in the transport of iron.. J Biol Chem 272:11770–11777
    [Google Scholar]
  7. Emery T. 1987; Reductive mechanisms of iron assimilation.. In Iron Transport in Microbes, Plants and Animals pp. 235–250 Winkelmann G., van der Helm D., Neilands J.B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
  8. Georgatsou E., Alexandraki D. 1994; Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccbaromyces cerevisiae. . Mol Cell Biol 14:3065–3073
    [Google Scholar]
  9. Goffeau A., Park J., Paulsen I.T., Jonniaux J.L., Dinh T., Mordant P., Saier M.H. 1997; Multidrug -resistant transport proteins in yeast: complete inventory and phylogenetic characterization of yeast open reading frames with the major facilitator superfamily.. Yeast 13:43–54
    [Google Scholar]
  10. van der Helm D., Winkelmann G. 1994; Hydroxamates and polycarboxylates as iron transport agents (siderophores) in fungi.. In Metal Ions in Fungi pp. 39–98 Winkelmann G., Winge D.R. Edited by New York, Basel & Hong Kong: Marcel Dekker;
    [Google Scholar]
  11. Lesuisse E., Labbe P. 1989; Reductive and non-reductive mechanisms of iron assimilation by the yeast Saccbaromyces cerevisiae. . J Gen Microbiol 135:257–263
    [Google Scholar]
  12. Lesuisse E., Labbe P. 1995; Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccbaromyces cerevisiae. . Microbiology 141:2937–2943
    [Google Scholar]
  13. Lesuisse E., Raguzzi F., Crichton R. 1987; Iron uptake by the yeast Saccbaromyces cerevisiae: involvement of a reduction step.. J Gen Microbiol 133:3228–3236
    [Google Scholar]
  14. Lesuisse E., Casteras-Simon M., Labbe P. 1995; Ferrireductase activity in Saccbaromyces cerevisiae and other fungi: colorimetric assays on agar plates.. Anal Biochem 226:375–377
    [Google Scholar]
  15. Morrissey J.A., Williams P.H., Cashmore A.M. 1996; Candida albicans has a cell-associated ferric-reductase activity which is regulated in response to levels of iron and copper.. Microbiology 142:485–492
    [Google Scholar]
  16. Nelissen B., De Wachter R., Goffeau A. 1997; Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccbaromyces cerevisiae. . FEMS Microbiol Rev 21:113–134
    [Google Scholar]
  17. Powell P.E., Cline G.R., Reid C.P.P., Szanislo J. 1980; Occurrence of hydroxamate siderophore iron chelates in soils.. Nature 287:833–835
    [Google Scholar]
  18. Roman D.G., Dancis A., Anderson G.I., Klausner R.D. 1993; The fission yeast ferric reductase gene frpl + is required for ferric iron uptake and encodes a protein that is homologous to the gp91- phox subunit of the human NADPH phagocyte oxidoreductase.. Mol Cell Biol 13:4342–1350
    [Google Scholar]
  19. Stearman R., Yuan D.S., Yamaguchi-lwai Y., Klausner R.D., Dancis A. 1996; A permease-oxidase complex involved in high- affinity iron uptake in yeast.. Science 271:1552–1557
    [Google Scholar]
  20. Wiebe C., Winkelmann G. 1975; Kinetic studies on the specificity of chelate-iron uptake in Aspergillus. . J Bacteriol 123:837–842
    [Google Scholar]
  21. Winkelmann G., Huschka H.G. 1987; Molecular recognition and transport of siderophores in fungi.. In Iron Transport in Microbes, Plants and Animals pp. 317–336 Winkelmann G., van der Helm D., Neilands J.B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
  22. Yamaguchi-lwai Y., Stearman R., Dancis A., Klausner R.D. 1996; Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast.. EMBO J 15:3377–3384
    [Google Scholar]
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