1887

Abstract

Summary: The AIDS-associated is capable of inducing its own uptake by non-phagocytic cells. The ability of to both adhere to and invade Molt-3 lymphocytes was markedly increased in the presence of polyethylene glycol 8000 (PEG). The effect of PEG was more pronounced in the more alkaline pH range, where the binding kinetics were much faster and almost unaffected by temperature (4-37 C). Incubation of [C]oleic-acid-labelled Molt-3 cells with viable resulted in a substantial release of radioactive fatty acids, whereas treating the host cells with heat-inactivated mycoplasmas, isolated membrane preparations, or growth medium, had no effect. Total lipid analysis of Molt-3 lymphocytes infected by revealed an augmented level of the neutral lipid fraction that was associated with a decrease in the relative amounts of polar lipids, mainly a decrease in the amount of phosphatidylserine and diphosphatidylglycerol. Analysis of the neutral lipid fraction in the infected Molt-3 cells revealed a fivefold increase in the relative amount of diacylglycerol and a marked increase in the free fatty acid (FFA) fraction. The profile of the FFAs released was dominated by a relatively high concentration of the polyunsaturated fatty acid docosahexaenoic acid. The release of lipid intermediates suggests that the degradation of Molt-3 cell phospholipids induced by may initiate a signal transmission cascade in the host cell.

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1998-12-01
2021-10-19
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References

  1. Andreev J., Borovsky Z., Rosenshine I., Rottem S. 1995; Invasion of HeLa cells by Mycoplasma penetrans and the induction of tyrosine phosphorylation of a 145 kDa host cell protein.. FEMS Lett 132:189–194
    [Google Scholar]
  2. Balsinde J., Diez E., Mollinedo F. 1991; Arachidonic acid release from diacylglycerol in human neutrophils.. J Biol Cbem 266:15638–15643
    [Google Scholar]
  3. Baseman J.B., Lange M., Criscimagna N.L., Giron J.A., Thomas C.A. 1995; Interplay between mycoplasmas and host target cells.. Microb Patbog 19:105–116.?
    [Google Scholar]
  4. Bligh E.G., Dyer W.J. 1959; A rapid method of total lipid extraction and purification.. Can J Biochem Physiol 37:911–917
    [Google Scholar]
  5. Borovsky Z., Tharshis M., Zhang P., Rottem S. 1998; Protein kinase C activation and vacuolation in HeLa cells invaded by Mycoplasma penetrans.. J Med Microbiol 47:915–922
    [Google Scholar]
  6. Brenner C., Neyrolles O., Blanchard A. 1996; Mycoplasmas and HIV infection: from epidemiology to their interaction with immune cells.. Front Biosci 1:e42–54
    [Google Scholar]
  7. Christie W.W. 1989 Gas Chromatography and Lipids. Ayr:: The Oily Press.;
    [Google Scholar]
  8. Falkow S., Isberg R.R., Portnoy D.A. 1992; The interaction of bacteria with mammalian cells.. Annu Rev Cell Biol 8:333–363
    [Google Scholar]
  9. Giron J.A., Lange M., Baseman J.B. 1996; Adherence, fibronectin binding, and induction of cytoskeleton reorganization in cultural human cells by Mycoplasma penetrans.. Infect Immun 64:197–208
    [Google Scholar]
  10. Katan M.B., Deslypere J.P., van Birgelen A.P., Penders M., Zegwaard M. 1997; Kinetics of the incorporation of dietary fatty acids into serum cholesteryl esters, erythrocyte membranes, and adipose tissue: an 18-month controlled study.. J Lipid Res 38:2012–2022
    [Google Scholar]
  11. Liscowitch M. 1992; Crosstalk among multiple signal-activated phospholipases.. Trends Biochem Sci 17:393–398
    [Google Scholar]
  12. Lo S.-C. 1992; Mycoplasma and AIDS.. In Mycoplasmas: Molecular Biology and Pathogenesis pp. 525–548 Maniloff J., McElhaney R.N., Finch L.R., Baseman J.B. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  13. Lo S.-C., Hayes M.M., Kotani H., Pierce P.F., Wear D.J., Newton P.B., Tully J.G., Shih J.W. 1993; Adhesion onto and invasion into mammalian cells by Mycoplasma penetrans: a newly isolated mycoplasma from patients with AIDS.. Mod Pathol 6:276–280
    [Google Scholar]
  14. Lucy J.A., Ahkong Q.F. 1989; Membrane fusion. Fusogenic agents and osmotic forces.. Subcell Biochem 14:189–228
    [Google Scholar]
  15. Neyrolles O., Brenner C., Prevost M.-C., Fontaine T., Montagnier L., Blanchard A. 1996; Isolation and characterization of a novel capsule-like material from Mycoplasma penetrans.. IOM Lett 4:366–367
    [Google Scholar]
  16. Nir S., Klappe K., Hoekstra D. 1986; Mass action analysis of kinetics and extent of fusion between Sendai virus and phospholipid vesicles.. Biochemistry 25:8261–8266
    [Google Scholar]
  17. Nishizuka Y. 1992; Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C.. Science 228:607–614
    [Google Scholar]
  18. Razin S., Jacobs E. 1992; Mycoplasma adhesion.. J Gen Microbiol 138:407–422
    [Google Scholar]
  19. Razin S., Rottem S. 1976; Techniques for the manipulation of mycoplasma membranes.. In Biochemical Methods in Membrane Studies pp. 3–26 Maddy A.H. Edited by London:: Chapman & Hall.;
    [Google Scholar]
  20. Rottem S. 1980; Membrane lipids of mycoplasma.. Biochim Biophys Acta 604:65–90
    [Google Scholar]
  21. Rottem S. 1983; Characterization of membrane lipids.. In Methods in Mycoplasmology 1 pp. 269–275 Razin S., Tully J.G. Edited by New York:: Academic Press.;
    [Google Scholar]
  22. Rottem S., Markowitz O. 1979; Membrane lipids of Mycoplasma gallisepticum: a disaturated phosphatidylcholine and a phosphatidylglycerol with an unusual positional distribution of fatty acids.. Biochemistry 18:2930–2935
    [Google Scholar]
  23. Ruschkowsky S., Rosenshine I., Finlay B.B. 1992; Salmonella typhimurium induces an inositol phosphate flux in infected epithelial cells.. FEMS Lett 95:121–126
    [Google Scholar]
  24. Salman M., Rottem S. 1995; The cell membrane of Mycoplasma penetrans: lipid composition and phospholipase A1activity.. Biochim Biophys Acta 1235:369–377
    [Google Scholar]
  25. Shaw J.H., Falkow S. 1988; Model for invasion of human tissue culture cells by Neisseria gonorrhoeae.. Infect Immun 56:1625–1632
    [Google Scholar]
  26. Shibata K.-I., Sasaki T., Watanabe T. 1995; AIDS-associated mycoplasmas possess phospholipase C in the membrane.. Infect Immun 63:4174–4177
    [Google Scholar]
  27. Wick M.J., Madara J.L., Fields B.N., Normark S.J. 1991; Molecular crosstalk between epithelial cells and pathogenic microorganisms.. Cell 67:651–659
    [Google Scholar]
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