1887

Abstract

Summary: The temperate bacteriophage VWB integrates into the chromosome of ETH14630 via site-specific integration. Following recombination of the VWB region with the chromosomal sequence, the host-phage junctions and are formed. Nucleotide sequence analysis of and revealed a 45 bp common core sequence. In this 45 bp sequence consists of the 3' end of a putative tRNA(AGG) gene with a 3'-terminal CCA sequence which is typical for prokaryotic tRNAs. Phage DNA integration restores the putative tRNA(AGG) gene in . However, following recombination the CCA sequence is missing as is the case for most tRNA genes described so far. Adjacent to VWB , an ORF encoding a 427 aa protein was detected. The C-terminal region of this protein shows high similarity to the conserved C-terminal domain of site-specific recombinases belonging to the integrase family. To prove the functionality of this putative integrase gene , an integrative vector pKT02 was constructed. This vector consists of a 23 kb III-l restriction fragment of VWB DNA containing and cloned in a non-replicative vector carrying a thiostrepton-resistance gene. Integration of pKT02 was obtained after transformation of ETH14630 and TK24 protoplasts. This vector will thus be useful for a number of additional species in which a suitable tRNA gene can be functional as integration site.

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/content/journal/micro/10.1099/00221287-144-12-3351
1998-12-01
2021-04-21
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References

  1. Abremski K.E., Hoess R.H. 1992; Evidence for a second conserved arginine residue in the integrase family of recombination proteins.. Protein Eng 5:87–91
    [Google Scholar]
  2. Anné J., Wohlleben W., Burkardt H.J., Springer R., Pü;hler A. 1984; Morphological and molecular characterization of several actinophages isolated from soil which lyse Streptomyces cattleya or S. venezuelae. . J Gen Microbiol 130:2639–2649
    [Google Scholar]
  3. Anné J., Van Mellaert L., Eyssen H. 1990a; Determination of the packaging capacity of bacteriophage VWB.. J Bacteriol 172:1129–33
    [Google Scholar]
  4. Anné J., Van Mellaert L., Decock B., Van Damme J., Van Aerschot A., Herdewijn P., Eyssen H. 1990b; Further biological and molecular characterization of actinophage VWB.. J Gen Microbiol 136:1365–33
    [Google Scholar]
  5. Anné J., Van Mellaert L., Eyssen H. 1990c; Optimum conditions for efficient transformation of Streptomyces vene-zuelae protoplasts.. Appl Microbiol Biotechnol 32:431–33
    [Google Scholar]
  6. Argos P., Landy A., Abremski K. 9otherauthors 1986; The integrase family of site-specific recombinases: regional similarities and global diversity.. EMBO J 5:433–33
    [Google Scholar]
  7. Bar-Nir D., Cohen A., Goedeke M.E. 1992; tDNAser sequences are involved in the excision of Streptomyces griseus plasmid pSGl.. Gene 122:71–76
    [Google Scholar]
  8. Boccard F., Pernodet J.-L., Friedmann A., Guérineau M. 1988; Site-specific integration of plasmid pSAM2 in Streptomyces lividans and S. ambofaciens. . Mol Gen Genet 212:432–439
    [Google Scholar]
  9. Boccard F., Smokvina T., Pernodet J.-L., Friedmann A., Guérineau M. 1989; The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages.. EMBO J 8:973–980
    [Google Scholar]
  10. Brasch M.A., Pettis G.S., Lee S.C., Cohen S.N. 1993; Localization and nucleotide sequences of genes mediating site- specific recombination of the SLP1 element in Streptomyces lividans. . J Bacteriol 175:3067–3074
    [Google Scholar]
  11. Brown D.P., Idler K.B., Katz L. 1990; Characterization of the genetic elements required for site-specific integration of plasmid pSE211 in Saccbaropolyspora erytbraea. . J Bacteriol 172:1877–33
    [Google Scholar]
  12. Brown D.P., Idler K.B., Backer D.M., Donadio S., Katz L. 1994; Characterization of the genes and attachment sites for site-specific integration of plasmid pSElOl in Saccbaropolyspora erytbraea and Streptomyces lividans. . Mol Gen Genet 242:185–193
    [Google Scholar]
  13. Chang A.C.Y., Cohen S.V. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the pl5A cryptic miniplasmid.. J Bacteriol 134:1141–1156
    [Google Scholar]
  14. Engler-Blum G., Meier M., Frank J., Müller G.A. 1993; Reduction of background problems in nonradioactive Northern and Southern blot analyses enables higher sensitivity than 32P- based hybridizations.. Anal Biochem 210:235–33
    [Google Scholar]
  15. Esposito D., Scocca J.J. 1997; The integrase family of tyrosine recombinases: evolution of a conserved active site domain.. Nucleic Acids Res 25:3605–3614
    [Google Scholar]
  16. Gabriel K., Schmid H., Schmidt U., Rausch H. 1995; The actinophage RP3 DNA integrates site-specifically into the putative tRNAArg(AGG) gene of Streptomyces rimosus. . Nucleic Acids Res 23:58–63
    [Google Scholar]
  17. Hauser M.A., Scocca i.J. 1992; Site-specific integration of the Haemophilus subluenzae bacteriophage HP1. Identification of the points of the recombinational exchange and the limits of the host attachment site.. J Biol Chem 267:6859–33
    [Google Scholar]
  18. Hoeltke H.-J., Schneider S., Ettl I., Binsack R., Obermaier I., Seller M., Sagner G. 1995; Rapid, highly sensitive detection of digoxigenin-labeled nucleic acids by improved chemiluminescent alkaline phosphatase substrates.. Biochemica (Boehringer) 1:17–20
    [Google Scholar]
  19. Hopwood D.A., Bibb M.J., Chater K.F. 7 other authors 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  20. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T. 1987; Plasmid and phage vectors for gene cloning and analysis in Streptomyces. . Methods Enzymol 153:116–166
    [Google Scholar]
  21. Katz E., Thompson C.J., Hopwood D.A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. . J Gen Microbiol 129:2703–2714
    [Google Scholar]
  22. Kendall K., Cullum J. 1986; Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2).. Mol Gen Genet 202:240–33
    [Google Scholar]
  23. Korn F., Weingärtner B., Kutzner H.J. 1978; A study of twenty actinophages: morphology, serological relationship and host range.. In Genetics of the Actinomycetales pp. 251–270 Edited by Freerksen E., Tarnok I., Thumin H. Stuttgart & New York: G. Fisher;
    [Google Scholar]
  24. Kuhstoss S., Rao R.N. 1991; Analysis of the integration function of the Streptomycete bacteriophage ɸC31.. J Mol Biol 222:897–908
    [Google Scholar]
  25. Kuhstoss S., Richardson M.A., Rao R.N. 1991; Plasmid cloning vectors that integrate site-specifically in Streptomyces spp.. Gene 97:143–146
    [Google Scholar]
  26. Leschziner A.E., Boocock M.R., Grindley N.D.F. 1995; The tyrosine-6 hydroxyl of yd resolvase is not required for the DNA cleavage and rejoining reactions.. Mol Microbiol 15:865–870
    [Google Scholar]
  27. Marsh J.L., Erfle M., Wykes E.J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.. Gene 32:481–485
    [Google Scholar]
  28. Matsuura M., Noguchi T., Yamaguchi D., Aida T., Asayama M., Takahashi H., Shirai M. 1996; The sre gene (ORF469) encodes a site-specific recombinase responsible for integration of the R4 phage genome.. J Bacteriol 178:3374–33
    [Google Scholar]
  29. Mazodier P., Thompson C., Boccard F. 1990; The chromosomal integration site of the Streptomyces element pSAM2 overlaps a putative tRNA gene conserved among actinomycetes.. Mol Gen Genet 222:431–434
    [Google Scholar]
  30. Nunes-Düby S.E., Kwon H.J., Tirumalai R.S., Ellenberger T., Landy A. 1998; Similarities and differences among 105 members of the Int family of site-specific recombinases.. Nucleic Acids Res 26:391–33
    [Google Scholar]
  31. Reiter W.-D., Palm P., Yeats S. 1989; Transfer RNA genes frequently serve as integration sites for prokaryotic genetic elements.. Nucleic Acids Res 17:1907–1914
    [Google Scholar]
  32. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Sedlmeier R., Werner T., Kieser H.M., Hopwood D.A., Schmieger H. 1994; tRNA genes of Streptomyces lividans: new sequences and comparison of structure and organization with those of other bacteria.. J Bacteriol 176:5550–5553
    [Google Scholar]
  34. Shirai M., Nara H., Sato A., Aida T., Takahashi H. 1991; Site- specific integration of the actinophage R4 genome into the chromosome of Streptomyces parvulus upon lysogenization.. J Bacteriol 173:4237–4239
    [Google Scholar]
  35. Sosio M., Madon J., Hütter R. 1989; Excision of pIJ408 from the chromosome of Streptomyces glaucescens and its transfer into Streptomyces lividans. . Mol Gen Genet 218:169–176
    [Google Scholar]
  36. Sprinzl M., Dank N., Nock J., Schdn A. 1991; Compilation of tRNA sequences and sequences of tRNA genes.. Nucleic Acids Res 19:2127–2171
    [Google Scholar]
  37. Vögtli M., Cohen N. 1992; The chromosomal integration site for Streptomyces plasmid SLP1 is a functional tRNATyr gene essential for cell viability.. Mol Microbiol 6:3041–3050
    [Google Scholar]
  38. Wertman K.F., Wyman A.R., Botstein D. 1986; Host/vector interactions which affect the viability of recombinant phage lambda clones.. Gene 49:253–262
    [Google Scholar]
  39. Yang W., Mizuuchi K. 1997; Site-specific recombination in plane view.. Structure 5:1401–1406
    [Google Scholar]
  40. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
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