Summary: The gene in (previously known as ) WSM419, directly downstream from , is induced by low pH or certain stresses (e.g. high concentrations of Zn, Cu, HO or ethanol), but not in stationary phase or by other stresses (e.g. phosphate limitation, elevated temperature, high concentrations of sucrose or iron). A DNA fragment containing the wild-type gene could not be cloned and inverse PCR was therefore used to amplify a 3·5 kb HI fragment containing from the mutant TG2-6 (::Tn5). DNA fragments from a HI/I digest of the amplified product were cloned into pUK21 and sequenced. The open reading frame contiguous to appears to code for a 15·2 kDa protein showing significant identity with the proteins encoded by y4wC and y4aM in sp. NGR234. All three proteins resemble transcriptional regulators in containing a DNA-binding helix-turn-helix motif similar to that reported for URF4 in and repressors in coliphage.


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