RT Journal Article SR Electronic(1) A1 Gu, Wei A1 Song, Jian A1 Bonner, Carol A. A1 Xie, Gang A1 Jensen, Roy A.YR 1998 T1 PhhC is an essential aminotransferase for aromatic amino acid catabolism in Pseudornonas aeruginosa JF Microbiology, VO 144 IS 11 SP 3127 OP 3134 DO https://doi.org/10.1099/00221287-144-11-3127 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The phhC gene of Pseudomonas aeruginosa encodes a protein which is a member of the Family I aminotransferases. At high expression levels in the heterologous Escherichia oli system, PhhC can compensate for the absence of AspC (which functions in L-aspartate biosynthesis) and TyrB (which functions in aromatic biosynthesis). In the native organism, PhhC is essential for catabolism of either L-tyrosine or L-phenylalanine, as demonstrated by gene inactivation. This catabolic function of PhhC is consistent with its inclusion as the distal gene in the inducible phenylalanine hydroxylase operon. The presence of PhhC for catabolism of aromatic amino acids is required in spite of an existing multiplicity of other P. aeruginosa aminotransferases having a similar pattern of broad substrate specificity in vitm. This implies a spatial orientation of PhhC that effectively specializes it for aromatic amino acid catabolism., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-11-3127