1887

Abstract

SUMMARY: All known gerF mutations affecting Bacillus subtilis spore germination have been mapped, by a combination of recombination and complementation analysis, to yvoC (/gt)# a gene belonging to the yvoB CptsK) yvoC (/gt) yvoDEF operon. Examination of the properties of null mutants confirmed that the only gene in the operon that affects germination is poC, which encodes a homologue of known prelipoprotein diacylglyceryl transferases. As several germination proteins (GerAC, GerBC, GerKC, GerD) are predicted lipoproteins, it is not unreasonable to assume that a defect in prelipoprotein processing will affect spore germination. Two other null mutants in this chromosomal region showed a clear phenotype: the nagA gene is required for growth on N-acetylglucosamine, whereas a null mutation in yvoB (ptsK) affects colony formation from single cells.

Keyword(s): gerF , lgt , lipoproteins , spore germination and yvoC
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/content/journal/micro/10.1099/00221287-144-11-3105
1998-11-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-11-3105
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