Truncation as a novel form of variation of the p50 gene in Mycoplasma hominis Free

Abstract

SUMMARY: A characteristic feature of the mycoplasmas is the presence of variable surface proteins which may play an important role in the adaptation of the cell-wall-less organisms to their host environments. In addition, this antigen variation may be an important pathogenic property of the organism. The ubiquity of the gene encoding P50, an adhesin of Mycop/asrna hominis FBG, and its transcription were analysed in different isolates ofM. horninis. The p50 gene was present in all isolates tested. Based on Southern blot analysis and sequencing of the gene, the isolates could be classified into one of three distinct groups. Within two groups specific truncations of the p50 gene occurred. The reduction of the gene size was confirmed in Northern blot analysis of representative isolates from each group, with a decrease in transcript length from 1.6 kb in group G-1 down to 076 kb in group 6-3. In addition to truncation, a coincidental duplication of some gene segments was detected. This work has provided evidence for the genetic basis of a further variation in the M. horninis P50 adhesin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-11-2979
1998-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/11/mic-144-11-2979.html?itemId=/content/journal/micro/10.1099/00221287-144-11-2979&mimeType=html&fmt=ahah

References

  1. Caruthers M.H. 1982; New methods for synthesizing deoxy- oligonucleotides.. Genet Eng 4:1–16
    [Google Scholar]
  2. Christiansen G. 1992; Genetic variation in natural populations.. In My coplasmas: Molecular Biology and Pathogenesis pp. 561–571 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  3. Christiansen G., Ladefoged S., Hange S., Birkelund S., Andersen H. 1990; Use of monoclonal antibodies for detection of gene and antigen variation in Mycoplasma hominis. . Zentbl Bakteriol (Suppl.) 20:535–545
    [Google Scholar]
  4. Feldmann R.-C., Henrich B., Kolb-Bachofen V., Hadding U. 1992; Decreased metabolism and viability of Mycoplasma hominis induced by monoclonal antibody-mediated agglutination.. Infect Immun 60:166–174
    [Google Scholar]
  5. Henrich B., Feldmann R.-C., Hadding U. 1993; Cytoadhesins of Mycoplasma hominis. . Infect Immun 61:2945–2951
    [Google Scholar]
  6. Henrich B., Kitzerow A., Feldmann R.-C., Schaal H., Hadding U. 1996; Repetitive elements of the Mycoplasma hominis adhesin P50 can be differentiated by monoclonal antibodies.. Infect Immun 64:4027–4034
    [Google Scholar]
  7. Higgins D.G., Sharp P.M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer.. Comput Appl Biosci 5:151–153
    [Google Scholar]
  8. Krause D.C., Taylor-Robinson D. 1992; Mycoplasmas which infect humans.. In Mycoplasmas: Molecular Biology and Pathogenesis pp. 417–444 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  9. Lüneberg E., Kamla V., Hadding U., Frosch M. 1991; Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu.. Gene 102:123–127
    [Google Scholar]
  10. Mardh P., Moller B.R., McCormarck W.M. 1983; M.hominis:a human pathogen.. Sex Transm Dis 10:4
    [Google Scholar]
  11. Olson L. D., Shane S. W., Karpas A. A., Cunningham T. M., Probst P. S., Barile M. F. 1991a; Monoclonal antibodies to surface antigens of a pathogenic Mycoplasma hominis strain.. Infect Immun 59:1683–1689
    [Google Scholar]
  12. Olson L. D., Renshaw C. A., Shane S. W., Barile M. F. 1991b; Successive synovial Mycoplasma hominis isolates exhibit antigenic variation.. Infect Immun 59:3327–3329
    [Google Scholar]
  13. Rosengarten R., Wise K. 1990; Phenotypic switching in mycoplasmas: phase variation of diverse surface lipoproteins.. Science 247:315–318
    [Google Scholar]
  14. Rosengarten R., Wise K. 1991; The vlp system of M.hyorhinis: combinatorial expression of distinct size variant lipoproteins generating high-frequency surface antigenic variation.. J Bacteriol 173:4782–4793
    [Google Scholar]
  15. Saito N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745436–5467
    [Google Scholar]
  18. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. J Mol Biol 98:503–517
    [Google Scholar]
  19. Wise K.S., Yogev D., Rosengarten R. 1992; Antigenic variation.. In Mycoplasmas: Molecular Biology and Pathogenesis pp. 473–489 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  20. Zhang Q., Wise K. 1996; Molecular basis of size and antigenic variation of a Mycoplasma hominis adhesin encoded by divergent vaa genes.. Infect Immun 64:2737–2744
    [Google Scholar]
  21. Zhang Q., Wise K. 1997; Localized reversible frameshift mutation in an adhesin gene confers a phase-variable adherence phenotype in mycoplasma.. Mol Microbiol 25:859–869
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-11-2979
Loading
/content/journal/micro/10.1099/00221287-144-11-2979
Loading

Data & Media loading...

Most cited Most Cited RSS feed