1887

Abstract

SUMMARY: Divercin V41 is a new bacteriocin produced by Carnobacterium divergens V41, a lactic acid bacterium isolated from fish viscera. The amino acid sequence of divercin V41 showed high homologies with pediocin PA-1 and enterocin A. Two disulphide bonds were present in the hydrophilic N-terminal domain and in the highly variable hydrophobic C-terminal domain, respectively. A DNA probe designed from the N-terminal sequence of the purified peptide was used to locate the structural gene of divercin V41. A 6 kb chromosomal fragment containing the divercin V41 structural gene (dvnA) was cloned and sequenced. The results indicate that divercin V41 is synthesized as a pre-bacteriocin of 66 amino acids. The 23-residue N-terminal extension is cleaved off t o yield the mature 43-amino-acid divercin V41. In addition, the fragment encodes putative proteins commonly found within bacteriocin operons, including an ATP- dependent transporter, two immunity-like proteins and the two components of a lantibiotic-type signal-transducing system. The genetic organization of the fragment suggested important gene rearrangements.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-10-2837
1998-10-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/10/mic-144-10-2837.html?itemId=/content/journal/micro/10.1099/00221287-144-10-2837&mimeType=html&fmt=ahah

References

  1. Axelsson L., Hoick A. 1995; The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.. J Bacteriol 177:2125–2137
    [Google Scholar]
  2. Aymerich T., Holo H., Håvarstein L.S., Hugas M., Garriga M., Nes I.F. 1996; Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new anti-listerial bacteriocin in the pediocin family of bacteriocins.. Appl Environ Microbiol 62:1676–1682
    [Google Scholar]
  3. van Belkum M.J., Stiles M.E. 1995; Molecular characterization of genes involved in the production of the bacteriocin leucocin A from Leuconostoc gelidum.. Appl Environ Microbiol 61:3573–3579
    [Google Scholar]
  4. van Belkum M.J., Kok J., Venema G., Holo H., Nes I.F., Konings W.N., Abee T. 1991; The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner.. J Bacteriol 173:7934–7941
    [Google Scholar]
  5. Bhugaloo-Vial P., Dousset X., Métivier A., Sorokine O., Anglade P., Boyaval P., Marion D. 1996; Purification and amino acid sequences of piscicocins V1a and V1b, two class IIa bacteriocins secreted by Carnobacterium piscicola V1 that display significantly different levels of specific inhibitory activity.. Appl Environ Microbiol 62:4410–4416
    [Google Scholar]
  6. Bruno M.E.C., Montville T.J. 1993; Common mechanistic action of bacteriocins from lactic acid bacteria.. Appl Environ Microbiol 59:3003–3010
    [Google Scholar]
  7. Chikindas M.J., Garcia-Garcera M.J., Driessen A.M., Leder-boer A.M., Nissen-Meyer J., Nes I.F., Abee T., Konings W.N., Venema G. 1993; Pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells.. Appl Environ Microbiol 59:3577–3584
    [Google Scholar]
  8. De Man J.C., Rogosa M., Sharpe E. 1960; A medium for the cultivation of Lactobacilli.. J Appl Bacteriol 23:130–135
    [Google Scholar]
  9. Diep D.B., Håvarstein L.S., Nes I.F. 1996; Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11.. J Bacteriol 178:4472–4483
    [Google Scholar]
  10. Entian K.D., de Vos W.M. 1996; Genetics of subtilin and nisin biosynthesis.. Antonie Leeuwenhoek 69:109–117
    [Google Scholar]
  11. Fath M.J., Kolter R. 1993; ABC transporters: bacterial exporters.. Microbiol Rev 57:995–1017
    [Google Scholar]
  12. Fremaux C, Héchard Y., Cenatiempo Y. 1995; Mesentericin Y105 gene clusters in Leuconostoc mesenteroides Y105.. Microbiology 141:1637–1645
    [Google Scholar]
  13. Hanahan D. 1983; Studies on transformation of Escherichia coliwith plasmid DNA.. J Mol Biol 166:557–580
    [Google Scholar]
  14. Hastings J.W., Stiles M.E. 1991; Antibiosis of Leuconostoc gelidum isolated from meat.. J Appl Bacteriol 70:127–134
    [Google Scholar]
  15. Hastings J.W., Sailer M., Johnson K., Roy K.L., Vederas J.C., Stiles M.E. 1991; Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum.. J Bacteriol 173:7491–7500
    [Google Scholar]
  16. Håvarstein L.S., Holo H., Nes I.F. 1994; The leader peptide of colicin V shares consensus sequences with leader peptides that are common amongst peptide bacteriocins produced by Gram-positive bacteria.. Microbiology 140:2383–2389
    [Google Scholar]
  17. Héchard Y., Derijard B., Letellier F., Cenatiempo Y. 1992; Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides.. J Gen Microbiol 138:2725–2731
    [Google Scholar]
  18. von Heijne G. 1990; The signal peptide.. J Membr Biol 115:195–201
    [Google Scholar]
  19. Higgins C.F., Hiles I.D., Salmond G.P. 7 other authors 1986; A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria.. Nature 323:448–450
    [Google Scholar]
  20. Hoch J.A., Silhavy T.J. (editors) 1995 Two-Component Signal Transduction. Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  21. Hoick A., Axelsson L., Birkeland S.E., Aukrust T. 1992; Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.. J Gen Microbiol 138:2715–2720
    [Google Scholar]
  22. Hühne K., Axelsson L., Hoick A., Krockel L. 1996; Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strains.. Microbiology 142:1437–1448
    [Google Scholar]
  23. Jack R.W., Tagg J.R., Ray B. 1995; Bacteriocins of gram-positive bacteria.. Microbiol Rev 59:171–200
    [Google Scholar]
  24. Jaquinod M., Potier N., Klarskov K. 7 other authors 1993; Sequence of pig lens aldose reductase and electrospray mass spectrometry of non-covalent and covalent complexes.. Eur J Biochem 218:893–903
    [Google Scholar]
  25. Jimenez-Diaz R., Rios-Sanchez R.M., Desmazeaud M., Ruiz-Barba J.L., Piard J.-C. 1993; Plantaricin S and T, two new bacteriocins produced by Lactobacillus plantarum LPC010 isolated from a green olive fermentation.. Appl Environ Microbiol 59:1416–1424
    [Google Scholar]
  26. Klaenhammer T.R. 1993; Genetics of bacteriocins produced by lactic acid bacteria.. FEMS Microbiol Rev 12:39–85
    [Google Scholar]
  27. Marugg J.D., Gonzales C.F., Kunka B.S., Ledeboer A.M., Pucci M.J., Toonen M.Y., Walker S.A., Zoetmulder L.C.M., Vandenbergh P.A. 1992; Cloning, expression and nucleotide sequence of genes involved in production of pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0.. Appl Environ Microbiol 58:2360–2367
    [Google Scholar]
  28. Muriana P.M., Klaenhammer T.R. 1987; Conjugal transfer of plasmid-encoded determinants for bacteriocin production and immunity in Lactobacillus acidophilus.. Appl Environ Microbiol 53:553–560
    [Google Scholar]
  29. Muriana P.M., Klaenhammer T.R. 1991; Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088.. Appl Environ Microbiol 57:114–121
    [Google Scholar]
  30. Nes I.F., Diep D.B., Håvarstein L.S., Brurberg M.B., Eijsink V., Holo H. 1996; Biosynthesis of bacteriocins in lactic acid bacteria.. Antonie Leeuwenhoek 70:113–128
    [Google Scholar]
  31. Piard J.-C., Muriana P.M., Desmazeaud M., Klaenhammer T.R. 1992; Purification and partial characterization of lacticin 481, a lanthionine containing bacteriocin produced by Lacto- coccus lactis subsp. lactis CNRZ 481.. Appl Environ Microbiol 58:279–284
    [Google Scholar]
  32. Pilet M.-F., Dousset X., Barre R., Novel G., Desmazeaud M., Piard J.-C. 1995; Evidence of two bacteriocins produced by Carnobacterium piscicola and Carnobacterium divergens isolated from fish and active against Listeria monocytogenes.. J Food Prot 58:256–262
    [Google Scholar]
  33. Quadri L.E.N., Sailer M., Roy K.L., Vederas J.C., Stiles M.E. 1994; Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17 B.. J Biol Chem 269:12204–12211
    [Google Scholar]
  34. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  35. Sanger F., Nicklen S., Coulson R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  36. Schillinger U., Geisen R., Holzapfel W.H. 1996; Potential of antagonistic microorganisms and bacteriocins for the biological preservation of foods.. Trends Food Sci Tech 7:158–164
    [Google Scholar]
  37. Scopes R.K. 1988 Protein Purification. Principles and Practice. New York:: Springer.;
    [Google Scholar]
  38. Stoddard G.W., Petzel J.P., van Belkum M.J., Kok J., McKay L.L. 1992; Molecular analyses of the lactococcin A gene cluster from Lactococcus lactis subsp. lactis biovar diacetylactis WM4.. Appl Environ Microbiol 58:1952–1961
    [Google Scholar]
  39. Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P. 1992; Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from Lactobacillus sake LTH673.. Syst Appl Microbiol 15:460–468
    [Google Scholar]
  40. Venema K., Kok J., Marugg J.D., Toonen M.Y., Ledeboer A.M., Venema G., Chikindas M.L. 1995; Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1.0: PedB is the immunity protein and PedD is the precursor processing enzyme.. Mol Microbiol 17:515–522
    [Google Scholar]
  41. de Vos W.M., Kuipers O.P., van der Meer J.R., Siezen R.J. 1995; Maturation pathway of nisin and other lantibiotics: post- translationally modified antimicrobial peptides exported by gram-positive bacteria.. Mol Microbiol 17:427–437
    [Google Scholar]
  42. Worobo R.W., van Belkum M.J., Sailer M., Roy K.L., Vederas J.C., Stiles M.E. 1995; A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens.. J Bacteriol 177:3143–3149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-10-2837
Loading
/content/journal/micro/10.1099/00221287-144-10-2837
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error