1887

Abstract

SUMMARY: The Streptomyces ghanaensis plasmid pSG5 is self-transmissible but does not form the growth-retardation zones (pocks) normally characteristic of the Streptomyces plasmid-transfer process. The complete nucleotide sequence of pSG5 was determined on both strands. pSG5 is 12208 bp in length and has a GC content of 68 mol0/o. Characterization’ of the open reading frames by insertion and deletion analysis revealed that only a single gene, traB, is involved in the transfer of pSG5. The deduced amino acid sequence of TraB is similar to the SpolllE protein that is responsible for chromosome translocation during prespore formation of Bacillus subtilis. In contrast to the tra genes of the other Streptomyces plasmids, the pSG5 traB does not represent a kill function. Although pSG5 transfer is not associated with pock formation, pSG5 was shown to possess putative spd genes that are responsible for the pock phenotype of other Streptomyces plasmids. However, promoter-probe experiments revealed that the spd genes of pSG5 are not transcribed, thus explaining the deficiency in pock formation.

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1998-10-01
2024-12-06
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References

  1. Allison S.L., Phillips A.T. 1990; Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida.. J Bacteriol 172:5470–5476
    [Google Scholar]
  2. Altschul S.F., Gish W., Miller W., Myers E.W., Lipmann D.J. 1990; Basic local alignment search tool.. J Mol Biol 215:403–410
    [Google Scholar]
  3. Begg K.J., Dewar S.J., Donachie W.D. 1995; A newEscherichia coli cell division gene, ftsK.. J Bacteriol 177:6211–6222
    [Google Scholar]
  4. Bibb M.J., Hopwood D.A. 1981; Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in Streptomyces coelicolor A3(2).. J Gen Microbiol 126:427–442
    [Google Scholar]
  5. Bibb M.J., Freeman R.F., Hopwood D.A. 1977; Physical and genetical characterization of a second sex factor, SCP2, for Streptomyces coelicolor A3(2).. Mol Gen Genet 154:155–166
    [Google Scholar]
  6. Bullock W.O., Fernandez J.M., Short J.M. 1987; XLl-Blue, a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.. Focus 5:376–378
    [Google Scholar]
  7. Clewell D.B. 1993; Sex pheromones and the plasmid encoded mating response in Enterococcus faecalis.. In Bacterial Conjugation, pp. 349–367 Clewell D.B. Edited by New York: Plenum;
    [Google Scholar]
  8. Click E.M., Webster R.E. 1998; The TolQRA proteins are required for membrane insertion of the major capsid protein of the filamentous phage fl during infection.. J Bacteriol 180:1723–1728
    [Google Scholar]
  9. Climo M.W., Sharma V.K., Archer G.L. 1996; Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the staphylococcal plasmid pGOl.. J Bacteriol 178:4975–4983
    [Google Scholar]
  10. Frost L.S., Ippen-lhler K., Skurray R.A. 1994; Analysis of the sequence and gene products of the transfer region of the F sex factor.. Microbiol Rev 58:162–210
    [Google Scholar]
  11. Gross J.D., Caro L.G. 1966; DNA transfer in bacterial conjugation.. J Mol Biol 16:269–284
    [Google Scholar]
  12. Haase J., Lurz R., Grahn M., Bamford D.H., Lanka E. 1995; Bacterial conjugation mediated by plasmid RP4: RSF1010 mobi lization, donor specific phage propagation, and pilus production require the same Tra2 core components of the proposed DNA transport complex.. J Bacteriol 177:4779–4791
    [Google Scholar]
  13. Hagège J., Pernodet J.-L., Sezonov G., Gebraud C., Friedmann A., Guèrineau M. 1993; Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer.. J Bacteriol 175:5529–5538
    [Google Scholar]
  14. Haydon D.J., Guest J.R. 1991; A new family of bacterial regulatory proteins.. FEMS Microbiol Lett 63:291–295
    [Google Scholar]
  15. Hernando Y., Palacios J.M., Imperial J., Ruiz-Argueso T. 1995; The bypBFCDE operon from Rhizobium leguminosarum biovar viciae is expressed from an Fnr-type promoter that escapes mutagenesis of the fnrN gene.. J Bacteriol 177:5661–5669
    [Google Scholar]
  16. Hofmann K., Stoffel W. 1993; TMbase - a database of membrane spanning proteins segments.. Biol Chem Hoppe-Seyler 347:166
    [Google Scholar]
  17. Hopwood D.A., Kieser T. 1993; Conjugative plasmids of Streptomyces. . In Bacterial Conjugation pp. 293–311 Clewell D.B. Edited by New York: Plenum;
    [Google Scholar]
  18. Hopwood D.A., Kieser T., Wright H.M., Bibb M.J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans TK66.. J Gen Microbiol 129:2257–2265
    [Google Scholar]
  19. Ilyina T., Koonin E.V. 1992; Conserved sequence motifs in the initiator proteins for rolling-circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria.. Nucleic Acids Res 20:3279–3285
    [Google Scholar]
  20. Kataoka M., Kiyose Y.M., Michisuji Y., Horiguchi T., Seki T., Yoshida T. 1994a; Complete nucleotide sequence of the Streptomyces nigrifaciens plasmid, pSN22: genetic organization and correlation with genetic properties.. Plasmid 32:55–69
    [Google Scholar]
  21. Kataoka M., Kosono S., Seki T., Yoshida T. 1994b; Regulation of the transfer genes of Streptomyces plasmid pSN22: in vivo and in vitro study of the interaction of TraR with promoter regions.. J Bacteriol 176:7291–7298
    [Google Scholar]
  22. Kendall K.J., Cohen S.N. 1987; Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJlOl.. J Bacteriol 169:4177–4183
    [Google Scholar]
  23. Kendall K.J., Cohen S.N. 1988; Complete nucleotide sequence of the Streptomyces lividans plasmid pIJlOl and correlation of the sequence with genetic properties.. J Bacteriol 170:4634–4651
    [Google Scholar]
  24. Kieser T., Hopwood D.A., Wright H.M., Thompson C.J. 1982; pI J101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.. Mol Gen Genet 185:223–238
    [Google Scholar]
  25. Kingsman A., Willetts N. 1978; The requirements for conjugal DNA synthesis in the donor strain during F lac transfer.. J Mol Biol 122:287–300
    [Google Scholar]
  26. Kosono S., Kataoka M., Seki T., Yoshida T. 1996; The TraB protein, which mediates the intermycelial transfer of the Streptomyces plasmid pSN22, has a functional NTP-binding motif and is localized to the cytoplasmic membrane.. Mol Microbiol 19:397–405
    [Google Scholar]
  27. Levengood S.K., Beyer W.F. Jr Webster R.E. 1991; TolA: a membrane protein involved in colicin uptake contains an extended helical region.. Proc Natl Acad Sci USA 88:5939–5943
    [Google Scholar]
  28. Manning P., Achtmann M. 1979; Cell-to-cell interactions in conjugating Escherichia coli: the involvement of the cell envelope.. In Bacterial Outer Membranes: Biogenesis and Functions pp. 409–448 Inouye M. Edited by New York: Wiley;
    [Google Scholar]
  29. Muth G., Wohlleben W., PUhler A. 1988; The minimal replicon of the Streptomyces ghanaensis plasmid pSG5 identified by subcloning and Tn5 mutagenesis.. Mol Gen Genet 211:424–429
    [Google Scholar]
  30. Muth G., NuBbaumer B., Wohlleben W., POhler A. 1989; A vector system with temperature-sensitive replication for gene disruption and mutational cloning.. Mol Gen Genet 219:341–348
    [Google Scholar]
  31. Muth G., Farr M., Hartmann V., Wohlleben W. 1995; Streptomyces ghanaensis plasmid pSG5: nucleotide sequence analysis of the self-transmissible minimal replicon and characterization of the replication mode.. Plasmid 33:113–126
    [Google Scholar]
  32. Muth G., Frese D., Kleber A., Wohlleben W. 1997; Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity are viable.. Mol Gen Genet 255:420–428
    [Google Scholar]
  33. Pansegrau W., Balzer D., Kruft V., Lurz R., Lanka R. 1990; Invitro assembly of relaxosomes at the transfer origin of plasmid RP4.. Proc Natl Acad Sci USA 87:6555–6559
    [Google Scholar]
  34. Pearson W.R., Lipman D.J. 1988; Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 85:2444–2448
    [Google Scholar]
  35. Pettis G.S., Cohen S.N. 1994; Transfer of the pIJlOl plasmid in Streptomyces lividans requires a cis-acting function dispensable for chromosomal gene transfer.. Mol Microbiol 13:955–964
    [Google Scholar]
  36. Pettis G.S., Cohen S.N. 1996; Plasmid transfer and expression of the transfer (tra) gene product of plasmid pIJlOl are temporally regulated during the Streptomyces lividans life cycle.. Mol Microbiol 19:1127–1135
    [Google Scholar]
  37. Servin-Gonzales L., Sampieri A., Cabello J., Galvan L., Juarez V., Castro C. 1995; Sequence and functional analysis of the Streptomyces phaeochromogenes plasmid pJV 1 reveals a modular organization of Streptomyces plasmids that replicate by rolling- circle.. Microbiology 141:2499–2510
    [Google Scholar]
  38. Solenberg P.J., Baltz R.H. 1994; Hypertransposing deriva-tives of the streptomycete insertion sequence 1S493.. Gene 147:47–54
    [Google Scholar]
  39. Staden R., McLachlan A.D. 1982; Codon preference and its use in identifying protein coding regions in large DNA sequences.. Nucleic Acids Res 10:141–156
    [Google Scholar]
  40. Stein D.S., Kendall K.J., Cohen S.N. 1989; Identification and analysis of transcriptional regulatory signals for the kil and kor loci of Streptomyces plasmid pIJlOl.. J Bacteriol 171:5768–5775
    [Google Scholar]
  41. Thompson J.D., Higgins D.G., Gibson T.J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence align-ment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  42. Tomura T., Kishino H., Doi K., Hara T., Kuhara S., Ogata S. 1993; Sporulation-inhibitory gene in pock forming plasmid pSAl.l of Streptomyces azureus.. Biosci Biotechnol Biochem 57:438–443
    [Google Scholar]
  43. Volff J.N., Altenbuchner J. 1997; High frequency trans-position of the Tn5 derivative Tn5493 in Streptomyces lividans.. Gene 194:81–86
    [Google Scholar]
  44. Vrijbloed J.W., Van der Put N.M.J., Dijkhuizen L. 1995; Identification and functional analysis of the transfer region of plasmid pMEA300 of the methylotrophic actinomycete Amyco- latopsis methanolica.. J Bacteriol 177:6499–6505
    [Google Scholar]
  45. Walker J.E., Saraste M., Runswick M.J., Gay N.J. 1982; Distantly related sequences in the α- and β-subunits of ATP synthase, myosin kinases and other ATP-requiring enzymes and a common nucleotide binding fold.. EMBO J 1:945–951
    [Google Scholar]
  46. Ward J.M., Janssen G.R., Kieser T., Bibb M.J., Buttner M.J. 1986; Construction and characterization of a series of multicopy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator.. Mol Gen Genet 203:468–478
    [Google Scholar]
  47. Weiss A.A., Johnson F.D., Burns D.L. 1993; Molecular characterization of an operon required for pertussis toxin secretion.. Proc Natl Acad Sci USA 90:2970–2974
    [Google Scholar]
  48. Wilkins B., Lanka E. 1993; DNA processing and replication during plasmid transfer between gram-negative bacteria.. In Bacterial Conjugation pp. 105–136 Clewell D.B. Edited by New York: Plenum;
    [Google Scholar]
  49. Wohlleben W., Muth G. 1993; Streptomyces plasmid vectors.. In Plasmids pp. 147–175 Hardy K.G. Edited by Oxford: IRL Press;
    [Google Scholar]
  50. Wright F., Bibb M.J. 1992; Codon usage in the G-t-C rich Streptomyces genome.. Gene 113:55–65
    [Google Scholar]
  51. Wu L.J., Errington J. 1997; Septal localization of the SpoIIIE chromosome partitioning protein in Bacillus subtilis.. EMBO J 16:2161–2169
    [Google Scholar]
  52. Wu X., Roy K.L. 1993; Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts.. J Bacteriol 175:37–52
    [Google Scholar]
  53. Wu L.J., Lewis P.J., Allmansberger R., Hauser P.M., Errington J. 1995; A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.. Genes Dev 9:1316–1326
    [Google Scholar]
  54. Yu T.-W., Hopwood D.A. 1996; Ectopic expression of the Streptomyces coelicolor whiE genes for polyketide spore pigment synthesis and their interaction with the act genes for actinorhodin biosynthesis.. Microbiology 141:2779–2791
    [Google Scholar]
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