@article{mbs:/content/journal/micro/10.1099/00221287-144-10-2749, author = "BaAueIos, Maria A. and Sychrová, Hana and Bleykasten-Grosshans, Claudine and Souciet, Jean-Luc and Potier, Serge", title = "The Nhal antiporter of Saccharomyces cerevisiae mediates sodium and potassium eft: lux", journal= "Microbiology", year = "1998", volume = "144", number = "10", pages = "2749-2758", doi = "https://doi.org/10.1099/00221287-144-10-2749", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-10-2749", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Na'/H+ antiporter", keywords = "salt tolerance", keywords = "yeast", keywords = "K+/H+ antiporter", abstract = "SUMMARY: The NHAl gene of Saccharomyces cerevisiae, transcribed into a 3-5 kb mRNA, encodes a protein mediating Na+ and K+ efflux through the plasma membrane that is required for alkali cation tolerance at acidic pH. Deletion of the gene in a wild-type strain resulted in higher sensitivity to both K+ and Na+ at acidic pH. Measurements of cation loss in strains carrying deleted or overexpressed alleles of NHAl demonstrated its role in K+ and Na+ efflux. In addition, high K+ and Na+ efflux observed upon alkalinization of the cytoplasm implies a role of Nhalp in the regulation of intracellular pH. Moreover, the overexpression of ENA1 and NHAl genes in an enal-46-nhalb strain showed that the Nhal alkalication antiporter is responsible for growth on high concentrations of KCI and NaCl at acidic pH, and Ena alkali-cation ATPases are necessary at higher pH values. Both systems have a complementary action to maintain the intracellular steadystate concentration of K+ and Na+.", }