Summary: Analysis of the nucleotide sequence of the form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes ( and ) of the non-sulfur purple bacterium indicated that the deduced amino acid sequence of the large subunit was not closely homologous to the large subunit from related organisms. Indeed, phylogenetic analysis suggested that the large subunit protein (CbbL) more closely resembled the enzyme from αlβlγ purple bacteria and cyanobacteria and is within a ‘green-like’ radiation of the RubisCO phylogenetic tree, well separated from CbbL of the related organism A gene was discovered downstream of in , a gene arrangement which also appears to be limited to certain organisms containing a ‘green-like’ RubisCO. Upstream, and divergently transcribed from , is a gene ( ) that encodes a LysR-type transcriptional activator. Phylogenetic analysis of the deduced amino acid sequence of CbbR also suggests that this protein is quite distinct from the CbbR protein, and is even distinct from the previously described CbbR protein, the gene of which is upstream and divergently transcribed from the operon of Interestingly, CbbR is more closely related to CbbR from bacteria whose RubisCO falls within the ‘greenlike’ radiation of the CbbL tree. These studies suggest that the - genes were acquired by via horizontal gene transfer from a bacterial species containing a ‘green-like’ RubisCO.


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