1887

Abstract

Summary: Strain ATCC 39006 of makes the same carbapenem, (5)-carbapen-2-em-3-carboxylic acid (Car), as the strain GS101. Unlike , where the onset of production occurs in the late-exponential phase of growth in response to the accumulation of the small diffusible pheromone -(3-oxohexanoyl)-L-homoserine lactone (OHHL), in carbapenem is produced throughout the growth phase and does not appear to involve any diffusible pheromone molecule. Two cosmids capable of restoring antibiotic production in group I carbapenem mutants were isolated from an gene library. These cosmids were shown to contain a homologue of the gene, encoding a CarR protein with homology to the LuxR family of transcriptional regulators. The was subcloned and shown to be capable of complementing , in the absence of OHHL, an double mutant, releasing the heterologous host from pheromone dependence for carbapenem production. The apparent OHHL-independence of the CarR explains the constitutive nature of carbapenem production in this strain of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-1-201
1998-01-01
2019-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-1-201
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error