Summary: Strain ATCC 39006 of makes the same carbapenem, (5)-carbapen-2-em-3-carboxylic acid (Car), as the strain GS101. Unlike , where the onset of production occurs in the late-exponential phase of growth in response to the accumulation of the small diffusible pheromone -(3-oxohexanoyl)-L-homoserine lactone (OHHL), in carbapenem is produced throughout the growth phase and does not appear to involve any diffusible pheromone molecule. Two cosmids capable of restoring antibiotic production in group I carbapenem mutants were isolated from an gene library. These cosmids were shown to contain a homologue of the gene, encoding a CarR protein with homology to the LuxR family of transcriptional regulators. The was subcloned and shown to be capable of complementing , in the absence of OHHL, an double mutant, releasing the heterologous host from pheromone dependence for carbapenem production. The apparent OHHL-independence of the CarR explains the constitutive nature of carbapenem production in this strain of


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