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Volume 144, Issue 1

A pheromone-independent CarR protein controls carbapenem antibiotic synthesis in the opportunistic human pathogen Serratia marcescens Free

Abstract

Summary: Strain ATCC 39006 of makes the same carbapenem, (5)-carbapen-2-em-3-carboxylic acid (Car), as the strain GS101. Unlike , where the onset of production occurs in the late-exponential phase of growth in response to the accumulation of the small diffusible pheromone -(3-oxohexanoyl)-L-homoserine lactone (OHHL), in carbapenem is produced throughout the growth phase and does not appear to involve any diffusible pheromone molecule. Two cosmids capable of restoring antibiotic production in group I carbapenem mutants were isolated from an gene library. These cosmids were shown to contain a homologue of the gene, encoding a CarR protein with homology to the LuxR family of transcriptional regulators. The was subcloned and shown to be capable of complementing , in the absence of OHHL, an double mutant, releasing the heterologous host from pheromone dependence for carbapenem production. The apparent OHHL-independence of the CarR explains the constitutive nature of carbapenem production in this strain of

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  • Accepted:
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  • Published Online:
Keyword(s): carbapenem , LuxR homologue , N-acylhomoserine lactone , Serratia marcescens and β-lactam
© Society for General Microbiology 1998
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1998-01-01
2024-04-19
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A pheromone-independent CarR protein controls carbapenem antibiotic synthesis in the opportunistic human pathogen Serratia marcescens
Microbiology 144, 201 (1998); https://doi.org/10.1099/00221287-144-1-201
/content/journal/micro/10.1099/00221287-144-1-201
/content/journal/micro/10.1099/00221287-144-1-201
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