1887

Abstract

Summary: Putative peptide-synthetase-encoding DNA fragments were isolated from the A3(2) chromosome using a PCR-based approach and mapped to a single ∼ 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the locus had been cloned.

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1998-01-01
2021-07-30
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