Summary: RNA polymerase (RNAP) was purified from AM1 cells grown on methanol or on succinate. The β, β', α and ω subunits were approximately the same size as those of , and the identity of the ω subunit was confirmed by N-terminal sequence analysis. N-terminal sequence analysis suggested that two other polypeptides in the purified RNAP preparation might be σ factors, a 40 kDa polypeptide that shared identity with σ homologues, and a 97 kDa polypeptide that shared identity with σ homologues in other bacteria. The 97 kDa polypeptide did not cross-react with antibody to σ. The same complement of putative σ factors was found in RNAP purified from AM1 grown on succinate and those grown on methanol, indicating that no major methanol-inducible σ factor is present in this strain. Run-off assays showed that the purified RNAP was capable of initiating transcription specifically at the transcriptional start site of a methylotrophic gene, , which encodes the large subunit of methanol dehydrogenase and is found only in methylotrophic bacteria.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error