Summary: One-hundred-and-fifty-two isolates of the genus , collected from hot springs on four continents, were screened for evidence of the presence of the thermophilic Type II restriction endonuclease (T/CGA). The presence of isoschizomers of in 27 of the isolates, originating from hot springs in New Zealand, Iceland, USA, Japan, mainland Portugal and the island of Sao Miguel in the Azores, is reported. Six of the -containing isolates from diverse geographical locations, identified by means of DNA/DNA homology and 16S rRNA sequence alignment as belonging to the species and , were selected for comparative studies. The isoschizomers from each of the six isolates were partially purified. They differed in their magnesium ion requirements, isoelectric points, subunit molecular masses and thermal stability.


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