1887

Abstract

Summary: Strains of the new species exhibit physiological and biochemical features very similar to those of the other species of the complex. To define taxonomic criteria for easy identification of , the glycolipid patterns of the reference strains and of 32 environmental and clinical isolates were examined by TLC. It was concluded that all strains of smooth colony morphology contained species-specific alkali-labile glycoconjugates. Three different patterns were observed among the strains of the smooth colony type. Fractionation followed by conventional chemical analyses of the purified glycolipids showed the specific glycolipids to be lipooligosaccharides (LOS). The three LOS showed a similar fatty acid composition consisting of straight chain (dodeca-, tetradeca-, hexadecanoyl and hexadecenoyl) and methyl-branched (2,4-dimethyleicosanoyl and 2,4-dimethyleicosenoyl) fatty acyl substituents. The most commonly encountered LOS (present in 76% of the smooth strains) contained a tetraacylated pentasaccharide composed of four moles of glucose and one mole of a 2,4-di--methylhexose. A LOS composed of arabinose, glucose and mannose was present in 20% of the smooth strains, whereas the newly proposed type strain of (ATCC 49650) was the only strain that contained a LOS composed of glucose and galactose. Serological studies clearly differentiated most of the strains of from those of the other members of the complex, and demonstrated the existence of serovars within the former species. Altogether, these data confirm the validity of the new species but show ATCC 49651 to be the most representative strain.

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1998-01-01
2021-08-04
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References

  1. Ariza M. A., Martfn-Luengo F., Valero-Guillén P. L. 1994; A family of diacyltrehaloses isolated from Mycobacterium fortuitum. . Microbiology 140:1989–1994
    [Google Scholar]
  2. Band J. D., Ward J. I., Fraser D. W., Peterson N. J., Silcox V. A., Good R. C., Ostrey P. R., Kennedy J. 1982; Peritonitis due to a Mycobacterium chelonae-like organism associated with intermittent chronic peritoneal dialysis. J Infect Dis 145:9–17
    [Google Scholar]
  3. Belisle J. T., Brennan P. J. 1989; Chemical basis of rough and smooth variation in mycobacteria. J Bacteriol 171:3465–3470
    [Google Scholar]
  4. Besra G. S., McNeil M., Brennan P. J. 1992; Characterization of the specific antigenicity of Mycobacterium fortuitum. . Biochemistry 31:6504–6509
    [Google Scholar]
  5. Besra G. S., McNeil M. R., Khoo K. H., Dell A., Morris H. R., Brennan P. J. 1993; Trehalose-containing lipooligosaccharides of Mycobacterium gordonae: presence of a mono-O-methyltetra-O-acyltrehalose ʹcoreʹ and branching in the oligosaccharide backbone. Biochemistry 32:12705–12714
    [Google Scholar]
  6. Bolan G., Reingold A., L, Carson L. A. 10 other authors 1985; Infections with Mycobacterium chelonae in patients receiving dialysis and using processed hemodialyzers. J Infect Dis 152:1013–1019
    [Google Scholar]
  7. Bradbury J. H., Jenkins G. A. 1984; Determination of the structures of trisaccharides by 13C-NMR spectroscopy. Carbohydr Res 126:125–156
    [Google Scholar]
  8. Brennan P. J., Goren M. B. 1979; Structural studies on the type-specific antigens and lipids of the Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum serocomplex. J Biol Chem 254:4205–4211
    [Google Scholar]
  9. Brennan P. J., Heifets M., Ullom B. P. 1982; Thin-layer chromatography of lipid antigens as a means of identifying nontuberculous mycobacteria. J Clin Microbiol 15:447–455
    [Google Scholar]
  10. Brennan P. J., Hunter S. W., McNeil M., Chatterjee D., Daffe M. 1990; Reappraisal of the chemistry of mycobacterial cell walls, with a view to understanding the roles of individual entities in disease processes. In Microbial Determinants of Virulence and Host Response pp 55–75 Edited by Ayoub E. M., Cassell G. H., Branche W. C. Jr, Henry T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Daffé M., Lacave C., Lanéelle M. A., Gillois M., Lanéelle G. 1988; Polyphthienoyl trehaloses, glycolipids specific for virulent strains of the tubercle bacillus. Eur J Biochem 172:579–584
    [Google Scholar]
  12. Daffé M., McNeil M., Brennan P. J. 1991; Novel type-specific lipooligosaccharides from Mycobacterium tuberculosis. . Biochemistry 30:378–388
    [Google Scholar]
  13. Falkinham J. O. III 1994; Epidemiology of Mycobacterium avium infections in the pre- and post-HIV era. Res Microbiol 145:169–172
    [Google Scholar]
  14. Gautier L., López Marín L. M., Lanéelle M. A., Daffe M. 1992; Structure of mycoside F, a family of trehalose-containing glycolipids of Mycobacterium fortuitum. . FEMS Lett 98:81–88
    [Google Scholar]
  15. Goslee S., Rynearson T. K., Wolinsky E. 1976; Additional serotypes of Mycobacterium scrofulaceum, Mycobacterium gordonae, Mycobacterium marinum, and Mycobacterium xenopi determined by agglutination. Int J Syst Bacteriol 26:136–142
    [Google Scholar]
  16. Kiehn T. E., Edwards F. F., Brannen P. 7 other authors 1985; Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J Clin Microbiol 21:168–173
    [Google Scholar]
  17. Kirschner P., Kiekenbeck M., Meissner D., Wolters J., Böttger E. C. 1992; Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification. J Clin Microbiol 30:2772–2775
    [Google Scholar]
  18. Kusunoki S., Ezaki T. 1992; Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov. Int J Syst Bacteriol 42:240–245
    [Google Scholar]
  19. Lanéelle M. A., Silve G., López Marín L. M., Daffé M. 1996; Structures of the glycolipid antigens of members of the third biovariant complex of Mycobacterium fortuitum . Eur J Biochem 238:270–279
    [Google Scholar]
  20. Lemassu A., Lévy-Frébault V. V., Lanéelle M. -A., Daffé M. 1992; Lack of correlation between colony morphology and lipooligosaccharide content in the Mycobacterium tuberculosis complex. J Gen Microbiol 138:1535–1541
    [Google Scholar]
  21. Lévy-Frébault V., Daffé M., Goh K. S., Laneelle M. A., Asselineau C., David H. L. 1983; Identification of Mycobacterium fortuitum and Mycobacterium chelonei. . J Clin Microbiol 17:744–752
    [Google Scholar]
  22. Lévy-Frébault V., Grimont F., Grimont P. A. D., David H. L. 1986; Deoxyribonucleic acid and relatedness study of the Mycobacterium fortuitum-Mycobacterium chelonae complex. Int J Syst Bacteriol 36:458–460
    [Google Scholar]
  23. López Marín L. M., Lanéelle M. A., Promé, D., Daffé M., Lanéelle G., Promé J. C. 1991; Glycopeptidolipids from Mycobacterium fortuitum-. a variant in the structure of c-mycoside. Biochemistry 30:10536–10542
    [Google Scholar]
  24. López Marín L. M., Gautier N., Lanéelle M. -A., Silve G., Daffé M. 1994; Structures of the glycopeptidolipid antigens of Mycobacterium abscessus and Mycobacterium chelonae and possible chemical basis of the serological cross-reactions in the Mycobacterium fortuitum complex. Microbiology 140:1109–1118
    [Google Scholar]
  25. Lowry P. W., Beck-Sague C. M., Bland L. A., Aguero S. M., Arduino M. J., Minuth A. N., Murray R. A., Swenson J. M., Jarvis W. R. 1990; Mycobacterium chelonae infection among patients receiving high-flux dialysis in a hemodialysis clinic in California. J Infect Dis 161:85–90
    [Google Scholar]
  26. McClatchy J. K. 1981; The seroagglutination test in the study of nontuberculous mycobacteria. Rev Infect Dis 3:867–870
    [Google Scholar]
  27. McNeil M., Chatterjee D., Hunter S. W., Brennan P. J. 1989; Mycobacterial glycolipids: isolation, structures, antigenicity, and synthesis of neoantigens. Methods Enzymol 179:215–242
    [Google Scholar]
  28. Muñoz M., Julián E., Garcia-Barceló M., Ausina V., Luquin M. 1997; Easy differentiation of Mycobacterium mucogenicum from other species of the Mycobacterium fortuitum complex by thin-layer and gas chromatography of fatty esters and alcohols. J Chromatogr B Biomed Appl 689:341–347
    [Google Scholar]
  29. Ryhage R., Stenhagen E. 1960; Mass spectrometric studies. IV. Esters of monomethyl-substituted long chain carboxylic acids. Ark Kemi 15:291–304
    [Google Scholar]
  30. Sauton B. 1912; Sur la nutrition minérale du bacille tuberculeux. C R Acad Sci Ser III Sci Vie 155:860–863
    [Google Scholar]
  31. Sempere M. A., Valero-Guillén P. L., de Godos A., Martín-Luengo F. 1993; A triacyltrehalose containing 2-methyl-branched unsaturated fatty acyl groups isolated from Mycobacterium fortuitum. . J Gen Microbiol 139:585–590
    [Google Scholar]
  32. Shaefer W. B. 1979; Serological identification of atypical mycobacteria. Methods Microbiol 13:323–344
    [Google Scholar]
  33. Silcox V. A., Good R. C., Floyd M. M. 1981; Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol 14:686–691
    [Google Scholar]
  34. Springer B., Böttger E. C., Kirschner P., Wallace R. J. Jr 1995; Phylogeny of the Mycobacterium chelonae-like organism based on partial sequencing of the 16S rRNA gene and proposal of Mycobacterium mucogenicum sp. nov. Int J Syst Bacteriol 45:262–267
    [Google Scholar]
  35. Sweeley C. C., Bentley R., Makita M., Wells W. W. 1963; Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Am Chem Soc 85:2497–2507
    [Google Scholar]
  36. Tsang A. Y., Barr V. L., McClatchy J. K., Goldberg M., Drupa I., Brennan P. J. 1984; Antigenic relationships of the Mycobacterium fortuitum-Mycobacterium chelonae complex. Int J Syst Bacteriol 34:35–44
    [Google Scholar]
  37. Wallace R. J. Jr 1994; Recent changes in taxonomy and disease manifestations of the rapidly growing mycobacteria. Eur J Clin Microbiol Infect Dis 13:953–960
    [Google Scholar]
  38. Wallace R. J. Jr, Swenson J. M., Silcox V. A., Good R. C., Tschen J. A., Seabury Stone M. 1983; Spectrum of disease due to rapidly growing mycobacteria. Rev Infect Dis 5:657–679
    [Google Scholar]
  39. Wallace R. J. Jr, Brown B. A., Silcox V. A. 8 other authors 1991; Clinical disease, drug susceptibility, and biochemical patterns of the unnamed third biovariant complex of Mycobacterium fortuitum. . J Infect Dis 163:598–603
    [Google Scholar]
  40. Wallace R. J. Jr, Silcox V. A., Tsukamura M., Brown B. A., Kilburn J. O., Butler W. R., Onyi G. 1993; Clinical significance, biochemical features, and susceptibility patterns of sporadic isolates of the Mycobacterium chelonae-like organism. J Clin Microbiol 31:3231–3239
    [Google Scholar]
  41. Yakrus M. A., Good R. C. 1990; Geographic distribution, frequency, and specimen source of Mycobacterium avium complex serotypes isolated from patients with acquired immunodeficiency syndrome. J Clin Microbiol 28:926–929
    [Google Scholar]
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