1887

Abstract

Summary: Two regions with sizes 18 900 and 25400 bp, which join previously known contigs containing and genes near 235δ of the chromosome, were sequenced. Among others, two genes, which encode proteins homologous to RNA polymerase σ-factors, were identified within this region. The gene products designated SigV and SigZ, show the highest homology with σ-factors encoded by the gene of and (formerly ) of , correspondingly. All σ-factors which show statistically significant homology to SigV and SigZ, belong to the ECF (extracytoplasmic functions) subfamily. SigV and SigZ do not have N-terminal sequence which prevents such proteins from binding to DNA without RNA polymerase core enzyme.

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/content/journal/micro/10.1099/00221287-143-9-2939
1997-09-01
2024-04-23
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References

  1. Bolotin A., Sorokin A., Ehrlich S. D. 1996; Mapping of the 150 kb spoIIICpheA region of the Bacillus subtilis chromosome by using Long Accurate PCR and three yeast artificial chromosomes. Microbiology 142:3017–3020
    [Google Scholar]
  2. Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S. D. 1996; Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SPβ prophage: use of Long Accurate PCR and yeast artificial chromosomes for sequencing. Microbiology 142:3005–3015
    [Google Scholar]
  3. Dombroski A. J., Walter W. A., Record M. T., Siegele D. A., Gross C. A. 1992; Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell 70:501–512
    [Google Scholar]
  4. Dombroski A. J., Walter W. A., Gross C. A. 1993; Amino-terminal amino acids modulate sigma-factor DNA-binding activity. Genes Dev 7:2446–2455
    [Google Scholar]
  5. Haldenwang W. G. 1995; The sigma factors of Bacillus subtilis . Microbiol Rev 59:1–30
    [Google Scholar]
  6. van Hove B., Staudenmaier H., Braun V. 1990; Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12. J Bacteriol 172:6749–6758
    [Google Scholar]
  7. Huang X., Decatur A., Sorokin A., Helmann J. D. 1997; The Bacillus subtilis σX protein is an extracytoplasmic function σ factor contributing to survival at high temperature. J Bacteriol 179:2915–2921
    [Google Scholar]
  8. Itaya M. 1993; Physical map of the Bacillus subtilis 168 chromosome. In Bacillus subtilis and Other Gram-positive Bacteria pp. 463–472 Edited by Sonenshein A. L., Hoch J. A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Koster M., van Klompenburg W., Bitter W., Leong J., Weisbeek P. 1994; Role of the outer membrane ferric siderophore receptor PupB in signal transduction across the bacterial cell envelope. EMBO J 13:2805–2813
    [Google Scholar]
  10. Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. 1994; Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase σ factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci USA 91:7573–7577
    [Google Scholar]
  11. Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. 1996; Systematic sequencing of the 283 kb 210°–232° region of the Bacillus subtilis genome containing the skin element and many sporulation genes. Microbiology 142:3103–3111
    [Google Scholar]
  12. Moszer I., Kunst F., Danchin A. 1996; The European Bacillus subtilis genome sequencing project: current status and accessibility of the data from a new World Wide Web site. Microbiology 142:2987–2991
    [Google Scholar]
  13. Msadek T., Kunst F., Rapoport G. 1993; Two-component regulatory systems. In Bacillus subtilis and other Gram-positive Bacteria pp. 729–746 Edited by Sonenshein A. L., Hoch J. A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Ohmiya K., Tanaka T., Noguchi N., O'Hara K., Kono M. 1989; Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenyltransferase from Bacillus subtilis Marburg 168. Gene 78:377–378
    [Google Scholar]
  15. Parro V., San Román M., Galindo I., Purnelle B., Bolotin A., Sorokin A., Mellado R. P. 1997; A 23911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the lev operon. Microbiology 143:1321–1326
    [Google Scholar]
  16. Sorokin A., Zumstein E., Azevedo V., Ehrlich S. D., Serror P. 1993; The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci. Mol Microbiol 10:385–395
    [Google Scholar]
  17. Sorokin A., Azevedo V., Zumstein E., Galleron N., Ehrlich S. D., Serror P. 1996; Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome. Microbiology 142:2005–2016
    [Google Scholar]
  18. Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y. 1996; Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gntsacXY region. Microbiology 142:3113–3123
    [Google Scholar]
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