1887

Abstract

Summary: Macrorestriction fragment profile analysis by PFGE was used to distinguish intestinal spirochaetes, some of which were isolated from cases of swine dysentery and intestinal spirochaetosis in humans, pigs, mice, chickens and dogs. Macrorestriction fragment profiles using and restriction enzymes were produced and used in statistical analysis. This permitted the division of the isolates into two major clusters. One cluster contained isolates which were identified as and the second cluster contained isolates identified as by immunoblotting with species-specific mAbs. Both species contained sub-specific groups, although these rarely correlated with the source of the isolates. We conclude that PFGE is capable of sub-specific differentiation of intestinal spirochaetes, but that the current species contain a large variety of genotypes among which cross-species transmission may be feasible.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-9-2923
1997-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/9/mic-143-9-2923.html?itemId=/content/journal/micro/10.1099/00221287-143-9-2923&mimeType=html&fmt=ahah

References

  1. Atyeo R. F., Oxberry S. L., Hampson D. J. 1996; Pulsed-field gel electrophoresis for sub-specific differentiation of Serpulina pilosicoli (formerly ‘ Anguillina coliʼ). FEMS Microbiol Lett 141:77–81
    [Google Scholar]
  2. Barrett S. P. 1990; Intestinal spirochaetes in a Gulf Arab population. Epidemiol Infect 104:261–266
    [Google Scholar]
  3. Barrett S. P., Holton J. J. M., Hookey J. V., Costas M., Ganner M., Mundy R., Wright D. J. M. 1996; Heterogeneity of human intestinal spirochaetes demonstrated by one dimensional polyacrylamide gel electrophoresis of proteins visualized by 35S-methionine labelling and Coomassie blue staining. J Med Microbiol 45:6–9
    [Google Scholar]
  4. Clark J. D. 1984; Biology and diseases of other rodents. In Laboratory Animal Medicine pp. 183–205 Edited by Fox J. G., Cohen B. J., Loew F. M. Orlando, FL: Academic Press;
    [Google Scholar]
  5. Duhamel G. E., Muniappa N., Mathiesen M. R., Johnson J. L., Toth J., Elder R. O., Doster A. R. 1995; Certain canine weakly β-hemolytic intestinal spirochetes are phenotypically and geno-typically related to spirochetes associated with human and porcine intestinal spirochetes. J Clin Microbiol 33:2212–2215
    [Google Scholar]
  6. Elder R. O., Duhamel G. E., Schafer R. W., Mathiesen M. R., Ramanathan M. 1994; Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR. J Clin Microbiol 32:1497–1502
    [Google Scholar]
  7. Fournié-Amazouz E., Baranton G., Carlier J. P., Chambreuil G., Cohadon F., Collin P., Gougeon Jolivet A., Hermes I., Lemarie C., Saint Girons I. 1995; Isolation of intestinal spirochaetes from the blood of human patients. J Hosp Infect 30:160–162
    [Google Scholar]
  8. Grothues D., Tummler B. 1991; New approaches in genome analysis by pulsed-field gel electrophoresis: application to the analysis of Pseudomonas species. Mol Microbiol 5:2763–2776
    [Google Scholar]
  9. Harris D. L., Glock R. D., Christensen C. R., Kinyon J. M. 1972; Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Int Vet Med Small Anim Clin 67:61–64
    [Google Scholar]
  10. Hookey J. V., Barrett S. P., Reed C. S., Barber P. 1994; Phylogeny of human intestinal spirochaetes inferred from 16S rRNA sequence comparisons. FEMS Microbiol Lett 117:345–350
    [Google Scholar]
  11. Hovind-Hougen K., Birch-Anderson A., Henrik-Nielsen R., Orholm M., Pedersen J. O., Teglbjaerg P. S., Thaysen E. H. 1982; Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J Clin Microbiol 16:1127–1136
    [Google Scholar]
  12. ter Huurne A. 1993 Swine dysentery: pathogenesis and vaccine development. PhD thesis University of Utrecht;
    [Google Scholar]
  13. Koopman M. B. H. 1990 Characterization of derpulina hyodysenteriae periplasmic flagella and hemolysin. PhD thesis University of Utrecht;
    [Google Scholar]
  14. Koopman M. B. H., Baats E., de Leeuw O. S., van der Zeijst B., Kusters J. G. 1993; Molecular analysis of a flagellar core protein gene of Serpulina (Treponema) hyodysenteriae . J Gen Microbiol 139:1701–1706
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  16. Lee J. I., Hampson D. J. 1992; Intestinal spirochaetes colonizing Aborigines from communities in the remote north of Western Australia. Epidemiol Infect 109:133–141
    [Google Scholar]
  17. Lee J. I., Hampson D. J. 1994; Genetic characterization of intestinal spirochaetes and their association with disease. J Med Microbiol 40:363–371
    [Google Scholar]
  18. Lee B. J., Hampson D. J. 1995; A monoclonal antibody reacting with the cell envelope of spirochaetes isolated from cases of intestinal spirochaetosis in humans and pigs. FEMS Microbiol Lett 131:179–184
    [Google Scholar]
  19. Lee B. J., Hampson D. J. 1996; Production and characterization of a monoclonal antibody to Serpulina hyodysenteriae . FEMS Microbiol Lett 136:193–197
    [Google Scholar]
  20. Lee J. I., Hampson D. J., Lymbery A. J., Harders S. J. 1993a; The porcine intestinal spirochaetes: identification of new groups. Vet Microbiol 34:273–285
    [Google Scholar]
  21. Lee J. I., McLaren A. J., Lymbery A. J., Hampson D. J. 1993b; Human intestinal spirochetes are distinct from Serpulina hyodysenteriae . J Clin Microbiol 31:16–21
    [Google Scholar]
  22. Lemcke R. M., Bew J. 1984; Antigenic differences amongst isolates of Treponema hyodysenteriae . In Proceedings of the 8th Pig Veterinary Society Congress Ghent, Belgium; p. 183
    [Google Scholar]
  23. McLaren A. J., Trott D. J., Swayne D. E., Stouten berg J. W., Hampson D. J. 1994; Characterization of avian intestinal spirochaetes. Proceedings of the 45th North Central Avian Disease Conference Des Moines, IA, USA, p. 66.
    [Google Scholar]
  24. Maslow J. N., Slutsky A. M., Arbeit R. D. 1993; Application of pulsed field gel electrophoresis to molecular epidemiology. In Diagnostic Molecular Microbiology: Principles and Applications pp. 563–572 Edited by Persing D. H., Smith T. F., Tenover F. C., White T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Neef N. A., Lysons R. J., Trott D. J., Hampson D. J., Jones P. W., Morgan P. H. 1994; Pathogenicity of porcine intestinal spirochaetes in gnotobiotic pigs. Infect Immun 62:2395–2403
    [Google Scholar]
  26. Rainey P. B., Thompson I. P., Palleroni N. J. 1994; Genome and fatty acid analysis of Pseudomonas stutzeri . Int J Syst Bacteriol 44:54–61
    [Google Scholar]
  27. Sanna A., Dettori G., Agliano A. M., Branca A. M., Grillo R., Leone F., Rossi A., Parisi G. 1984; Studies of treponemes isolated from the gastrointestinal tract. Ig Mod 81:959–973
    [Google Scholar]
  28. Stanton T. B., Trott D. J., Lee J. I., McLaren A. J., Hampson D. J., Paster B. J., Jensen N. S. 1996; Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. FEMS Microbiol Lett 136:18–186
    [Google Scholar]
  29. Towbin H., Staehlin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
    [Google Scholar]
  30. Trott D. J., Stanton T. B., Jensen N. S., Duhamel G. E., Johnson J. L., Hampson D. J. 1996; Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochaetosis. Int J Syst Bacteriol 46:206–215
    [Google Scholar]
  31. Trott D. J., Jensen N. S., Saint Girons I., Oxberry S. L., Stanton T. B., Lindquist D., Hampson D. J. 1997; Identification and characterization of Serpulina pilosicoli isolates recovered from the blood of critically ill patients. J Clin Microbiol 35:482–485
    [Google Scholar]
  32. Zuerner R. L., Stanton T. B. 1994; Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome. J Bacteriol 176:1087–1092
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-9-2923
Loading
/content/journal/micro/10.1099/00221287-143-9-2923
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error