1887

Abstract

Summary: The gene of encodes the non-proton-translocating NADH dehydrogenase II. Expression of the gene is subject to a complex network of regulatory controls at the transcriptional level. Under anaerobic conditions is repressed by the regulator of fumarate and nitrate reduction (FNR). However, in the absence of FNR, expression is activated by the amino acid response regulator (Arr) during anaerobic growth in rich medium. Expression of the gene varies during the growth cycle in response to the intracellular concentration of the heat-stable DNA-binding protein, Fis. In this work two additional heat-stable proteins, integration host factor (IHF) and the histone-like protein HU were found to interact with the promoter. IHF was shown to bind at three sites centred at +26, -17 and -58 in the promoter ( = 10 M), to prevent open-complex formation and to repress transcription . Studies with an fusion confirmed that IHF represses expression . Two putative binding sites for Arr, which overlap the two FNR boxes in the promoter, were identified. Studies with the FNR-activated and amino-acid-inducible asparaginase II gene () showed that IHF and a component of the Arr-containing fraction (but not HU) interact with the corresponding promoter.

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1997-09-01
2021-05-06
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