%0 Journal Article %A Takeyama, Haruko %A Takeda, Daisuke %A Yazawa, Kazunaga %A Yamada, Akiko %A Matsunaga, Tadashi %T Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp. %D 1997 %J Microbiology, %V 143 %N 8 %P 2725-2731 %@ 1465-2080 %R https://doi.org/10.1099/00221287-143-8-2725 %K metabolic engineering %K conjugation %K Synechococcus sp. %K marine cyanobacterium %K eicosapentaenoic acid (EPA) production %I Microbiology Society, %X Summary: The eicosapentaenoic acid (EPA) synthesis gene cluster isolated from a marine bacterium, Shewanella putrefaciens strain SCRC-2738, was cloned and expressed in the marine cyanobacterium Synechococcus sp. A broad-host-range cosmid vector, pJRD215 (10.2 kb, Smr Kmr), was used to clone a 38 kb insert, pEPA, containing the EPA synthesis gene cluster, creating plasmid pJRDEPA (approx. 48 kb). This plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10−7. Cyanobacterial transconjugants grown at 29 °C produced 0.12 mg EPA (g dry weight)−1, whereas those grown at 23 °C produced 0.56 mg EPA (g dry weight)−1. The yield was further improved to 0.64 mg (g dry weight)−1 by incubation for 1 d at 17 °C. This is believed to be the first successful cloning and expression of such a large heterologous gene cluster in a marine cyanobacterium. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-8-2725