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Volume 143, Issue 8

The Sch9 protein kinase in the yeast controls cAPK activity and is required for nitrogen activation of the fermentable-growth-medium-induced (FGM) pathway Free

Abstract

Summary: In cells of the yeast , trehalase activation, repression of (catalase), (Hsp70) and other STRE-controlled genes, feedback inhibition of cAMP synthesis and to some extent induction of ribosomal protein genes is controlled by the Ras-adenylate cyclase pathway and by the fermentable-growth-medium-induced pathway (FGM pathway). When derepressed cells are shifted from a non-fermentable carbon source to glucose, the Ras-adenylate cyclase pathway is transiently activated while the FGM pathway triggers a more lasting activation of the same targets when the cells become glucose-repressed. Activation of the FGM pathway is not mediated by cAMP but requires catalytic activity of cAMP-dependent protein kinase (cAPK; Tpk1, 2 or 3). This study shows that elimination of Sch9, a protein kinase with homology to the catalytic subunits of cAPK, affects all target systems in derepressed cells in a way consistent with higher activity of cAPK In measurements with trehalase and kemptide as substrates confirmed that elimination of Sch9 enhances cAPK activity about two- to threefold, in both the absence and presence of cAMP. it similarly affected the basal and final level but not the extent of the glucose-induced responses in derepressed cells. The reduction in growth rate caused by delation of is unlikely to be responsible for the increase in cAPK activity since reduction of growth rate generally leads to lower cAPK activity in yeast. On the other hand, deletion of abolished the responses of the protein kinase A targets in glucose-repressed cells. Re-addition of nitrogen to cells starved for nitrogen in the presence of glucose failed to trigger activation of trehalase, caused strongly reduced and aberrant repression of and , and failed to induce the upshift in expression. From these results three conclusions can be drawn: (1) Sch9 either directly or indirectly reduces the activity of protein kinase A; (2) Sch9 is not required for glucose-induced activation of the Rasadenylate cyclase pathway; and (3) Sch9 is required for nitrogen-induced activation of the FGM pathway. The latter indicates that Sch9 might be the target of the FGM pathway rather than cAPK itself.

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Keyword(s): nutrient signalling , ribosomal protein genes , trehalase and yeast
© Society for General Microbiology 1997
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1997-08-01
2024-04-23
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The Sch9 protein kinase in the yeast Saccharomyces cerevisiae controls cAPK activity and is required for nitrogen activation of the fermentable-growth-medium-induced (FGM) pathway
Microbiology 143, 2627 (1997); https://doi.org/10.1099/00221287-143-8-2627
/content/journal/micro/10.1099/00221287-143-8-2627
/content/journal/micro/10.1099/00221287-143-8-2627
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