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Volume 143, Issue 8

Cadmium-specific formation of metal sulfide ‘Q-particles’ by Klebsiella pneumoniae Free

Abstract

Summary: overcomes cadmium toxicity through the ‘biotransformation’ of cadmium ions into photoactive, nanometre-sized CdS particles deposited on the cell surface. The kinetics of particle formation during batch culture growth was monitored by electron microscopy (EM), energy-dispersive X-ray analysis and electronic absorption spectroscopy (EAS). During the deceleration phase of bacterial growth, the presence of CdS particles on the outer cell wall of K. pneumoniae (≥ 5 nm in diameter) was detected by EM. The size of these electron-dense particles continued to increase throughout the stationary phase of growth, with some of the particles reaching a diameter >200 nm. The formation of the extracellular CdS particles contributed to around 3-4% of the total cell biomass. EAS undertaken on these extracellular ‘bio-CdS’ particles suggested that the large ‘superparticles’ observed by EM, e.g. 200 nm, were aggregates of smaller particles termed ‘Q-particles’, ~ 4 nm in diameter. Metal sulfide particles were not formed in batch cultures of K. pneumoniae grown in the presence of lead, zinc, mercury, copper or silver ions. Growth in the presence of lead ions resulted in the formation of extracellular electron-dense particles containing lead but not sulfide or phosphate. Intracellular phosphorus-containing electron-opaque particles were formed during growth in the presence of copper and mercury. Intracellular electron-dense particles were formed in the presence of zinc ions but these did not contain phosphorus. From these results it was thought that metal sulfide formation in showed some cadmium-specificity. When cadmium and zinc ions were both added to the growth medium, metal sulfide particles were formed that were predominantly composed of cadmium, e.g. 48.6% cadmium and 0.04% zinc. Similarly, when cadmium and lead ions were both present during growth only CdS particles formed. In both cases analysis of the cells by EAS confirmed the presence of CdS only. These observations suggest that the mechanism of CdS formation is unlikely to occur simply through a cadmium-induced release of hydrogen sulfide by the cells into the external environment. If hydrogen sulfide production was the mechanism of sulfide formation then metal sulfide particles containing lead and zinc ions in addition to cadmium ions should have been produced.

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Keyword(s): cadmium sulfide , energy-dispersive X-ray analysis , heavy metal resistance and Klebsiella pneumoniae
© Society for General Microbiology 1997
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1997-08-01
2024-04-23
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References

  1. Aiking H., Kok K., van Heerikhuizen H., van't Riet J. 1982; Adaptation to cadmium by Klebsiella aerogenes growing in continuous culture proceeds mainly via formation of cadmium sulfide. Appl Environ Microbiol 44:938–944
     [Google Scholar]
  2. Aiking H., Stijnman A., van Garderen, C, van Heerikhuizen H., van't Riet J. 1984; Inorganic phosphate accumulation and cadmium detoxification in Klebsiella aerogenes NCTC 418 growing in continuous culture. Appl Environ Microbiol 47:374–377
     [Google Scholar]
  3. Aiking H., Govers H., van't Riet J. 1985; Detoxification of mercury, cadmium and lead in Klebsiella aerogenes NCTC 418 growing in continuous culture. Appl Environ Microbiol 50:1262–1267
     [Google Scholar]
  4. Cunningham D. P., Lundie L. L. 1993; Precipitation of cadmium by Clostridium thermoaceticum. . Appl Environ Microbiol 59:7–14
     [Google Scholar]
  5. Fortin D., Southam G., Beveridge T. J. 1994; Nickel sulfide, iron-nickel sulfide and iron sulfide precipitation by a newly isolated Desulfotomaculum species and its relation to nickel resistance. FEMS Microbiol Ecol 14:121–132
     [Google Scholar]
  6. Gadd G. M., Griffiths A. J. 1978; Microorganisms and heavy metal toxicity. Microbiol Ecol 4:303–317
     [Google Scholar]
  7. Hayat M. A. 1989 Principles and Techniques of Electron Microscopy: Biological Applications, 3rd edn.. London: MacMillan;
     [Google Scholar]
  8. Henglein A. 1987; Fluorescence, photochemistry and size quantization effects of colloidal semiconductor particles. J Chem Phys 84:1043–1047
     [Google Scholar]
  9. Henglein A. 1989; Small-particle research: physiochemical properties of extremely small colloidal metal and semiconductor particles. Chem Rev 89:1861–1873
     [Google Scholar]
  10. Holmes J. D., Smith P. R., Evans-Gowing R., Richardson D. J., Russeii D. A., Sodeau J. R. 1995a; Energy-dispersive X-ray analysis of the extracellular cadmium sulfide crystallites of Klebsiella aerogenes. . Arch Microbiol 163:143–147
     [Google Scholar]
  11. Holmes J., D„ Smith P. R., Evans-Gowing R., Richardson D. J., Russell D. A., Sodeau J. R. 1995b; Bacterial photoprotection through extracellular cadmium sulfide crystallites. Photochem Pbotobiol 62:1022–1026
     [Google Scholar]
  12. Holmes J. D., Farrar J. A., Richardson D. J., Russell D. A., Sodeau J. R. 1997; Bacterial cadmium sulfide semiconductor particles: an assessment of their photoactivity by EPR spectroscopy. Photochem Photobiol 65:811–817
     [Google Scholar]
  13. Hughes M. N., Poole R. K. 1989 Metals and Microorganisms London: Chapman & Hall;
     [Google Scholar]
  14. Khazaeli M. B., Mitra R. S. 1981; Cadmium-binding component in Escherichia coli during accomodation to low levels of this ion. Appl Environ Microbiol 41:46–50
     [Google Scholar]
  15. King T. E., Morris R. O. 1967; Determination of acid-labile sulfide and thiol groups. Methods Enzymol 10:634–641
     [Google Scholar]
  16. Kredich N. M., Foote L. J., Keenan B. S. 1973; The stoichiometry and kinetics of the inducible cysteine desulfhydrase from Salmonella typhimurium. . J Biol Chem 218:6187–6196
     [Google Scholar]
  17. Kurek E., Francis A. J., Bollag J.-M. 1991; Immobilization of cadmium by microbial extracellular products. Arch Environ Contam Toxicol 20:106–111
     [Google Scholar]
  18. Ogawa S., Fan F.-R., F. & Bard A. J. 1995; Scanning tunneling microscopy, tunneling spectroscopy, and photoelectrochemistry of a film of Q-CdS particles incorporated in a self-assembled monolayer on a gold surface. J Phys Chem 99:11182–11189
     [Google Scholar]
  19. Reed S. J. B. 1993 Electron Microprobe Analysis, 2nd edn.. Cambridge: Cambridge University Press;
     [Google Scholar]
  20. Shriver D. F., Atkins P. W., Langford C. H. 1990 Inorganic Chemistry Oxford: Oxford University Press;
     [Google Scholar]
  21. Slawson R. M., Trevors J. T., Lee H. 1992; Silver accumulation and resistance in Pseudomonas stutzeri. . Arch Microbiol 158:398–404
     [Google Scholar]
  22. Slawson R. M., Lohmeier-Vogel E. M., Lee H., Trevors J. T. 1994; Silver resistance in Pseudomonas stutzeri. . Biometals 7:30–40
     [Google Scholar]
  23. Spanhel L., Haase M., Weller H., Henglein A. 1987; Photochemistry of colloidal semiconductors. 20. Surface modification and stability of strong luminescing CdS particles. J Am Chem Soc 109:5649–5655
     [Google Scholar]
  24. Vogel R., Hoyer P., Weller H. 1994; Quantum-sized PbS, CdS, Ag2S, Sb2S3 and Bi2S3 particles as sensitizers for various nano-porous wide-bandgap semiconductors. J Phys Chem 98:3183–3188
     [Google Scholar]
  25. Weller H. 1993; Colloidal semiconductor Q-particles: chemistry in the transition region between solid state and molecules. Angew Chem Int Ed Engl 32:41–53
     [Google Scholar]
  26. Weller H., Eychmuller A. 1995; Photochemistry and photo-electrochemistry of quantized matter: properties of semiconductor nanoparticles in solution and thin-film electrodes. . In Advances in Photochemistry pp. 165–216 . Edited by Neckers D. C., Volman D. H., von Bunau G. Chichester: Wiley;
     [Google Scholar]
  27. Yanagida S., Yoshiya M., Shiragami T., Pac C., Mori H., Fujita H. 1990; Semiconductor photocatalysis. Quantitative photo-reduction of aliphatic ketones to alcohols using defect-free ZnS quantum crystallites. J Phys Chem 94:3104–3111
     [Google Scholar]
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Cadmium-specific formation of metal sulfide ‘Q-particles’ by Klebsiella pneumoniae
Microbiology 143, 2521 (1997); https://doi.org/10.1099/00221287-143-8-2521
/content/journal/micro/10.1099/00221287-143-8-2521
/content/journal/micro/10.1099/00221287-143-8-2521
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