RT Journal Article SR Electronic(1) A1 Bickerdike, S. R. A1 Holt, R. A. A1 Stephens, G. M.YR 1997 T1 Evidence for metabolism of o-xylene by simultaneous ring and methyl group oxidation in a new soil isolate JF Microbiology, VO 143 IS 7 SP 2321 OP 2329 DO https://doi.org/10.1099/00221287-143-7-2321 PB Microbiology Society, SN 1465-2080, AB Summary: An o-xylene-utilizing Rhodococcus, strain B3, was isolated from enrichments with o-xylene. The pathway for o-xylene degradation was investigated by simultaneous adaptation experiments, studies of product formation by a mutant and fortuitous oxidation studies using trimethylbenzene isomers as substrates. Two pathways were found to operate simultaneously and both were inducible. The first pathway involved the oxidation of a methyl group to form 2-methylbenzyl alcohol, followed by oxidation via the corresponding acid to 3-methylcatechol. The second pathway involved oxidation of the aromatic ring to form a dimethylcatechol. The bulk of the evidence suggests that the initial reaction was catalysed by a monooxygenase rather than a dioxygenase, and that 2,3-dimethylphenol was produced as an intermediate., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-7-2321