1887

Abstract

Summary: Three monoclonal antibodies (mAbs), S4D1, S3B3 and S1E5, were produced from hybridoma cell limes raised from mice immunized with hyphal walls of and one (Pax-1) from mice immunized with hyphal walls of In immunofluorescence studies, the three mAbs recognized epitopes with different patterns of distribution at the hyphal surface of S4D1 recognized an epitope which was present on the surface of both conidia and hyphae; S3B3 recognized an epitope seen only at the ends of conidia or in the septal region of hyphae and conidial chains; and S1E5 recognized an epitope present on the surface of hyphae, but not on mature conidia. mAb Pax-1 reacted with hyphal wall fragments of and with conidia in a similar way to S3B3. S4D1 reacted with an epitope found in 1,3-α-glucan preparations from hyphal walls of different fungi. The surface distribution of this epitope varied: it was found of the surface of both conidia and hyphae of and on the basidiospore surface only of and on the hyphae but not the conidia of Immunogold studies revealed tha the epitope was present throughout the wall of conidia and hyphae of mAbs S3B3, S1E5 and Pax-1 also reacted with other fungi: for example Pax-1 cross-reacted with all fungi tested except for a member of the Zygomycota. Immunogold studies revealed that epitopes of these three mAbs were present within the inner layers of the walls of conidia and hyphae of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-7-2255
1997-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/7/mic-143-7-2255.html?itemId=/content/journal/micro/10.1099/00221287-143-7-2255&mimeType=html&fmt=ahah

References

  1. Banks J. N., Cox S. J. 1992; The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies. Mycopathologia 120:79–85
    [Google Scholar]
  2. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu Rev Microbiol 22:87–108
    [Google Scholar]
  3. Bendayan M. 1984; Enzyme-gold electron microscopic cyto-chemistry: a new affinity approach for the ultrastructural localization of macromolecules. J Electron Microsc Techn 1:349–372
    [Google Scholar]
  4. Benhamou N., Chamberland H., Noel S. 1990; Ultrastructural localization of β-l,4-glucan-containing molecules in the cell walls of some fungi: a comparative study between spore and mycelium. Can J Microbiol 36:149–158
    [Google Scholar]
  5. Breuil C., Luck B. T., Rossignol L., Little J., Echeverri J., Banerjee S., Brown D. L. 1992; Monoclonal antibodies to Gliocladium roseum, a potential biological control fungus of sap-staining fungi in wood. J Gen Microbiol 138:2311–2319
    [Google Scholar]
  6. Bull A. T. 1970; Chemical composition of wild-type and mutant Aspergillus nidulans cell walls. The nature of polysaccharide and melanin constituents. J Gen Microbiol 63:74–94
    [Google Scholar]
  7. Cardimil L., Pincheira G. 1979; Characterization of the carbohydrate component of Fraction I in the Neurospora crassa cell wall. J Bacteriol 137:1067–1072
    [Google Scholar]
  8. Chang P. K. Y., Trevithick J. P. 1972; Release of wall-bound invertase and trehalose in Neurospora crassa by hydrolytic enzymes. J Gen Microbiol 70:13–22
    [Google Scholar]
  9. Cole L., Hawes C., Dewey F. M. 1991; Immunocytochemical localization of a specific antigen in cell walls of Penicillium islandicum . Mycol Res 95:1369–1374
    [Google Scholar]
  10. Gianinazzi-Pearson V., Lemoine M.-C., Arnould C., Gollote A., Morton J. B. 1994; Localization of β (1 3) glucans in spore and hyphal walls of fungi in the Glomales. Mycologia 86:478–85
    [Google Scholar]
  11. Hardham A. R., Susaki E., Perkin J. L. 1985; The detection of monoclonal antibodies for specific components on the surface of zoospores and cysts of the fungus Phytophtbora cinnamomi . Exp Mycol 9:264–268
    [Google Scholar]
  12. Hardham A. R., Cahill D. M., Cope M., Gabor B. K., Gubler F., Hyde G. J. 1994; Cell surface antigens of Phytophtbora spores: biological and taxonomic characterization. Protoplasma 181:213–232
    [Google Scholar]
  13. Hunsley D., Burnett J. H. 1970; The ultrastructural architecture of walls of some hyphal fungi. J Gen Microbiol 62:203–218
    [Google Scholar]
  14. Hunsley D, Kay D. 1976; Wall structure of the Neurospora hyphal apex: immunofluorescent localization of wall surface antigens. J Gen Microbiol 95:233–248
    [Google Scholar]
  15. Jeffries P., Dodd J. C., Jeger M. J., Plumbley R. A. 1990; The biology and control of Colletotrichum species on tropical fruit crops. Plant Pathol 39:343–366
    [Google Scholar]
  16. Mahadevan P. R., Tatum E. L. 1965; Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol 90:1073–1081
    [Google Scholar]
  17. Marshall M. 1990 Immunological recognition of fungi PhD thesis University of Kent:
    [Google Scholar]
  18. Marx D. H. 1969; The influence of ectotropic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 59:153–163
    [Google Scholar]
  19. Miller S. A., Martin R. R. 1988; Molecular diagnosis of plant disease. Annu Rev Phytopathol 26:409–432
    [Google Scholar]
  20. Notermans S., Wieten G., Engel H. W. B., Rombouts F. M., Hoogerhout P., Van Boom J. H. 1987; Purification and properties of extracellular polysaccharide (EPS) antigens produced by different mould species. J Appl Bacteriol 62:157–166
    [Google Scholar]
  21. Notermans S., Veeneman G. H., Van Zuylen C. W. E., M., Hoogerhout P., Van Boom J. H. 1988; (1 → 5)-linked β-d-galactofuranosides are immunodominant in extracellular polysaccharides of Penicillium and Aspergillus species. Mol Immunol 25:975–979
    [Google Scholar]
  22. Ollert M. W., Calderone R. A. 1990; A monoclonal antibody that defines a surface antigen on Candida albicans hyphae cross-reacts with yeast cell protoplasts. Infect Immun 58:625–631
    [Google Scholar]
  23. Pain N. A., O’Connell R. J., Bailey J. A., Green J. R. 1992; Monoclonal antibodies which show restricted binding to four Colletotrichum species: C. lindemuthianum, C. malvarum, C. orbiculare and C. trifolii. . Physiol Mol Plant Pathol 41:111–126
    [Google Scholar]
  24. Pain N. A., Green J. R., Gammie F., O’Connell R. J. 1994a; Immunomagnetic isolation of viable intracellular hyphae of Colletotrichum lindemuthianum (Sacc. & Magn.) Briosi & Cav. from infected bean leaves using a monoclonal antibody. New Phytol 127:223–232
    [Google Scholar]
  25. Pain N. A., O’Connell R. J., Mendgen K., Green J. R. 1994b; Identification of glycoproteins specific to biotrophic intracellular hyphae formed in Colletotrichum lindemuthianum-bean interaction. New Phytol 127:233–242
    [Google Scholar]
  26. Ruiz-Herrera J. 1991; Biosynthesis of β-glucans in fungi. Antonie Leeuwenhoek 60:73–81
    [Google Scholar]
  27. Schots A., Dewey F. M., Oliver R. 1994 Modern Assays for Plant Pathogenic Fungi: Identification, Detection and Quantification Wallingford: CAB International;
    [Google Scholar]
  28. Springer M. L., Yanofsky C. 1989; A morphological and genetic analysis of conidiospore development in Neurospora crassa . Genes Dev 3:559–571
    [Google Scholar]
  29. Ste-Marie L., Senechal S., Bouschira M., Garzon S., Strykowski H., Pedneault L., Repentigny L. D. 1990; Production and characterization of monoclonal antibodies to cell wall antigens of Aspergillus fumigatus . Infect Immun 58:2105–2114
    [Google Scholar]
  30. Thornton C. R., Dewey F. M. 1996; Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay. Mycol Res 100:217–222
    [Google Scholar]
  31. Thornton C. R., Dewey F. M., Gilligan C. A. 1993; Development of monoclonal antibody-based immunological assays for the detection of live propagules of Rhizoctonia solani in soil. Plant Pathol 42:763–773
    [Google Scholar]
  32. Wycoff K. L., Ayers A. R. 1990; Monoclonal antibodies to surface and extracellular antigens of a fungal plant pathogen, Phytophthora megasperma f.sp. glycinea, recognise specific carbohydrate epitopes. Physiol Mol Plant Pathol 37:55–79
    [Google Scholar]
  33. Xia J. Q., Lee F. N., Scott H. A., Raymond L. R. 1992; Development of monoclonal antibodies specific for Pyricularia grisea, the rice blast pathogen. Mycol Res 96:867–873
    [Google Scholar]
  34. Xia J. Q., Lee F. N., Spiegel F. W. 1993; Characterization of monoclonal antibodies to conidial antigens of Pyricularia grisea . Exp Mycol 17:170–181
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-7-2255
Loading
/content/journal/micro/10.1099/00221287-143-7-2255
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error