RT Journal Article SR Electronic(1) A1 Wu, Guanghui A1 Hill, Susan A1 Kelly, Mark J. S. A1 Sawers, Gary A1 Poole, Robert K.YR 1997 T1 The cydR gene product, required for regulation of cytochrome bd expression in the obligate aerobe Azotobacter vinelandii, is an Fnr-like protein JF Microbiology, VO 143 IS 7 SP 2197 OP 2207 DO https://doi.org/10.1099/00221287-143-7-2197 PB Microbiology Society, SN 1465-2080, AB Summary: The cytochrome bd complex in the obligately aerobic diazotroph Azotobacter vinelandii is an oxidase, which, in vivo, has a low affinity for oxygen and is required for respiratory protection of nitrogenase. Mutations caused by insertion of Tn5-B20 upstream of the structural genes (cydAB) for cytochrome bd result in over-expression of this oxidase and, for unexplained reasons, inability of the organism to grow microaerobically. Cloning and sequencing of this upstream region revealed a gene, cydR. The deduced amino acid sequence of CydR indicates that it is a new member of the Fnr class of regulators and that it represses cydAB expression. Refined mapping data for three insertions in cydR are presented. The cloned cydR gene complemented anaerobic growth of Escherichia coli fnr mutants and strongly enhanced expression of a narG-lacZ fusion in an E. coli fnr mutant., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-7-2197