1887

Abstract

Summary: The gene of has been cloned and sequenced and its expression in and analysed. The same genomic organization to that in () has been found in The and genes cannot be transcribed from their own promoters in In addition to the well-known homology at the protein level between AlaS proteins from various organisms, a strong homology was found between all the known genes from bacteria, archaea and eucarya. Two regions, one of which corresponds to the catalytic core, are particularly well-conserved at the nucleotide sequence level, a possible indication of strong constraints during evolution on these parts of the genes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-7-2179
1997-07-01
2021-04-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/7/mic-143-7-2179.html?itemId=/content/journal/micro/10.1099/00221287-143-7-2179&mimeType=html&fmt=ahah

References

  1. Aiba H., Adhya S., de Combrugghe B. 1981; Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem 256:11905–11910
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1991 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  4. Blasco F., lobbi C., Giordano G., Chippaux M., Bonnefoy V. 1989; Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the α and β subunits in iron binding and electron transfer. Mol Gen Genet 218:249–256
    [Google Scholar]
  5. Brown L. D., Rawlings D. E. 1993 A comparison of the structure of the H+-translocating ATP synthase fromThiobacillus ferrooxidans with those from other organisms. . In Biohydro-metallurgical Technologies , pp. 519–528 . Edited by Torma A. E., Apel M. L., Brierley C. L. Warrendale, PA: Minerals, Metals and Materials Society;
    [Google Scholar]
  6. Brown L. D., Dennehy M. E., Rawlings D. E. 1994; The F1 genes of the F1F0, ATP synthase from the acidophilic bacterium Thiobacillus ferrooxidans complement Escherichia coli F1 unc mutants. FEMS Microbiol Lett 122:19–26
    [Google Scholar]
  7. Cairns-Smith A. G., Hall A. J., Russel M. J. 1992; Mineral theories of the origin of life and an iron sulfide example. Origins Life Evol Biosphere 22:161–180
    [Google Scholar]
  8. Chang P. K., Dignam J. D. 1990; Primary structure of alanyl-tRNA synthetase and the regulation of its mRNA level in Bombyx mori . J Biol Chem 265:20898–20906
    [Google Scholar]
  9. Chun C. T., Niemela S. L., Miller R. H. 1989; One step preparation of competent Escherichia coli. Transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175
    [Google Scholar]
  10. Davis M. W., Buechter D. D., Schimmel P. 1994; Functional dissection of a predicted class-defining motif in a class II tRNA synthetase of unknown structure. Biochemistry 33:9904–9911
    [Google Scholar]
  11. De Mot R., Schoofs G., Vanderleyden J. 1994; A putative regulatory gene downstream of recA is conserved in Gram-negative and Gram-positive bacteria. Nucleic Acids Res 7:1313–1314
    [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  13. Eriani G., Delarue M., Poch O., Gangloff J., Moras D. 1990; Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs. Nature 347:203–206
    [Google Scholar]
  14. Filley S. J., Hill K. A. W. 1993; Amino acid substitutions at position 73 in motif 2 of Escherichia coli alanyl-tRNA synthetase. Arch Biochem Biophys 307:46–51
    [Google Scholar]
  15. Fleischmann R. D., Adams M. D., White O. & 37 other authors 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512
    [Google Scholar]
  16. Fraser C. M., Gocayne J. D., White O. & 26 other authors 1995; The minimal gene complement of Mycoplasma genitalium . Science 270:397–403
    [Google Scholar]
  17. Fry I.J., Garcia E. 1989 Cloning and characterization of Thiobacillus ferrooxidans genes involved in sulfur assimilation. . In Biohydrometallurgy , pp. 172–185 . Edited by Saley J., McCready R. G. L., Wichlacz P. L. Ottawa:
    [Google Scholar]
  18. Guerry P., Pope P. M., Burr D. H., Leifer J., Joseph S. W., Bourgeois A. L. 1994; Development and characterization of recA mutants of Campylobacter jejuni for inclusion in attenuated vaccines. Infect Immun 62:426–432
    [Google Scholar]
  19. Guiliani N., Chippaux M., Patte J.-C., Bonnefoy V. 1993 Perspectives in the genetics of Thiobacillus ferrooxidans. . In Biohydrometallurgical Technologies , pp. 645–658 . Edited by Torma A. E., Apel M. L., Brierley C. L. Warrendale, PA: Minerals, Metals and Materials Society;
    [Google Scholar]
  20. Jasin M., Regan L., Schimmel P. 1983; A modular arrangement of functional domains along the sequence of an aminoacyl tRNA synthetase. Nature 306:441–447
    [Google Scholar]
  21. Leduc L. G., Ferroni G. D. 1994; The chemolithotrophic bacterium Thiobacillus ferrooxidans . FEMS Microbiol Lett 108:103–120
    [Google Scholar]
  22. Lu Y., Hill K. A. W. 1994; The invariant arginine in motif 2 of Escherichia coli alanyl tRNA synthetase is important for catalysis but not for substrate binding. J Biol Chem 269:12137–12141
    [Google Scholar]
  23. Martin B., Garcia P., Castanig M.-P., Claverys J.-P. 1995; The recA gene of Streptococcus pneumoniae is part of a competence- induced operon and controls lysogenic induction. Mol Microbiol 15:367–379
    [Google Scholar]
  24. Miller J. H. 1992 A Short Course in Bacterial Genetics: a Laboratory Manual and Handbook for Escherichia coli and Related Bacteria Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Miller W. T., Schimmel P. 1992; A retroviral-like metal binding motif in an aminoacyl-tRNA synthetase is important for tRNA recognition. Proc Natl Acad Sci USA 89:2032–2035
    [Google Scholar]
  26. Miller W. T., Hill K. A. W., Schimmel P. 1991; Evidence for a ‘cysteine-histidine box’ metal-binding site in an Escherichia coli aminoacyl-tRNA synthetase. Biochemistry 30:6970–6976
    [Google Scholar]
  27. Murphy R. C., Gasparich G. E., Bryant D. A., Porter R. D. 1990; Nucleotide sequence and further characterization of the Synechococcus sp. strain PC7002 recA gene: complementation of a cyanobacterial recA mutation by the Escherichia coli recA gene. J Bacteriol 172:967–976
    [Google Scholar]
  28. Nagel G. M., Doolittle R. F. 1991; Evolution and relatedness in two aminoacyl-tRNA synthetase families. Proc Natl Acad Sci USA 88:8121–8125
    [Google Scholar]
  29. Putney S. D., Schimmel P. 1981; An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature 291:632–635
    [Google Scholar]
  30. Putney S. D., Royal N. J., De Vegvar H. N., Herlihy W. C., Biemann K., Schimmel P. 1981; Primary structure of a large aminoacyl-tRNA synthetase. Science 213:1497–1501
    [Google Scholar]
  31. Ramesar R. S., Woods D. R., Rawlings D. E. 1988; Cloning and expression in Escherichia coli of a recA-like gene from the acidophilic autotroph Thiobacillus ferrooxidans . J Gen Microbiol 134:1141–1146
    [Google Scholar]
  32. Ramesar R. S., Abratt V., Woods D. R., Rawlings D. E. 1989; Nucleotide sequence and expression of a cloned Thiobacillus ferrooxidans recA gene in Escherichia coli . Gene 78:1–8
    [Google Scholar]
  33. Ramirez C., Schimmin L. C., Leggatt P., Matheson A. T. 1994; Structure and transcription of the L11-L1-L10-L12 ribosomal protein gene operon from the extreme thermophilic archaeon Sulfolobus acidocaldarius . J Mol Biol 244:242–249
    [Google Scholar]
  34. Rawlings D. E., Woods D. R., Mjoli N. P. 1991; The cloning and structure of genes from the autotrophic biomining bacterium Thiobacillus ferrooxidans . Adv Gene Technol 2:215–237
    [Google Scholar]
  35. Ribas de Pouplana L., Buechter D. D., Davis M. W., Schimmel P. 1993; Idiographic representation of conserved domain of a class II tRNA synthetase of unknown structure. Protein Sci 2:2259–2262
    [Google Scholar]
  36. Ripmaster T. L., Shiba K., Schimmel P. 1995; Wide crossspecies aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen. Proc Natl Acad Sci USA 92:4932–4936
    [Google Scholar]
  37. Salazar O., Takamiya M., Orellana O. 1989; Characterization of the two rRNA gene operons present in Thiobacillus ferrooxidans . FEBS Lett 176:4409–4415
    [Google Scholar]
  38. Salles C,, Créancier L., Claverys J., -P. & Méjean V. 1992; The high level streptomycin resistance gene from Streptococcus pneumoniae is homologous to the ribosomal protein S12 gene of E. coli . Nucleic Acids Res 20:6103
    [Google Scholar]
  39. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY; Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Sano Y. 1993; Role of the recA-related gene adjacent to the recA gene in Pseudomonas aeruginosa . J Bacteriol 175:2451–2454
    [Google Scholar]
  41. Schimmel P. 1990; Alanine transfer RNA synthetase: structure-function relationships and molecular recognition of transfer RNA. Adv Enzymol 63:233–270
    [Google Scholar]
  42. Schimmel P. 1991; Classes of aminoacyl-tRNA synthetases and the establishment of the genetic code. Trends Biochem Sci 16:1–3
    [Google Scholar]
  43. Schimmel P., Giegé R., Moras D., Yokohama S. 1993; An operational RNA code for amino acids and possible relationship to genetic code. Proc Natl Acad Sci USA 90:8763–8768
    [Google Scholar]
  44. Schmitt W., Odenbreit S., Heuermann D., Haas D. 1995; Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Mol Gen Genet 248:563–572
    [Google Scholar]
  45. Selbitschka W., Arnold W., Priefer U. B., Rottschafer T., Schmidt M., Simon R., PUhler A. 1991; Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae . Mol Gen Genet 229:86–95
    [Google Scholar]
  46. Shi J. P., Musier-Forsyth K., Schimmel P. 1994; Region of a conserved sequence motif in a class II tRNA synthetase needed for transfer of an activated amino acid to an RNA substrate. Biochemistry 33:5312–5318
    [Google Scholar]
  47. Shiba K., Ripmaster T., Suzuki N., Nichols R., Plotz P., Noda T., Schimmel P. 1995; Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition. Biochemistry 34:10340–10349
    [Google Scholar]
  48. Temple K. L., Colmer A. R. 1951; The autotrophic oxidation of iron by a new bacterium: Thiobacillus ferrooxidans . J Bacteriol 62:605–611
    [Google Scholar]
  49. Venegas A., Hevia E., Sanchez H. 1988; Sequence of two tRNA genes from a Thiobacillus ferrooxidans ribosomal operon. Nucleic Acids Res 16:8179
    [Google Scholar]
  50. Wilson R., Ainscough R., Anderson K. & 50 other authors 1994; 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans. Nature 368:32–38
    [Google Scholar]
  51. Wu M.-X., Filley S. J., Xiong J., Lee J. J., Hill K. A. W. 1994; A cysteine in the C-terminal region of alanyl tRNA synthetase is important for aminoacylation activity. Biochemistry 33:12260–12266
    [Google Scholar]
  52. Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark A. J. 1994; Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations. Mutat Res 323:173–177
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-7-2179
Loading
/content/journal/micro/10.1099/00221287-143-7-2179
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error