Summary: The tetracycline resistance determinant tet(S) was first detected in antibiotic multiresistant Listeria monocytogenes BM4210 and subsequently in strains of Enterococcus faecalis. Transfer of tet(S) from clinical isolate E. faecalis BM4242 to E. faecalis strains JH2-2 and OG1RF was found to require the presence in the donor strain of the 55 kb conjugative plasmid pIP825. Comparison of restriction endonuclease generated maps of the donor, the two recipients, and of four transconjugants indicated that transfer of tet(S) (i) was from chromosome to chromosome, (ii) resulted in the acquisition of an approximately 40 kb element in the same chromosomal region and (iii) was associated with the exchange of large chromosomal fragments. Similar observations were made following conjugal transfer of tet(S) from four other E. faecalis clinical isolates.
BedzykL. A.,
ShoemakerN. B.,
YoungK. E.,
SalyersA. A.1992; Insertion and excision of Bacteroides conjugative chromosomal elements. J Bacteriol 174:166–172
CharpentierE.,
GerbaudG.,
CourvalinP.1993; Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210. Gene 131:27–34
FrankeA. E.,
ClewellD. B.1981; Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of ‘conjugal’ transfer in the absence of a conjugative plasmid. J Bacteriol 145:494–502
FrankeA. E.,
DunnyG. M.,
BrownB. L.,
AnF.,
OliveD. R.,
DamleS. P.,
ClewellD. B.1978 Gene transfer in Streptococcus faecalis: evidence for the mobilization of chromosomal determinants by transmissible plasmids. . In Microbiology–1978 , pp. 45–47 . Edited by
SchlessingerD.
Washington, DC: American Society for Microbiology;
GaillardJ. L.,
BercheP.,
SansonettiP.1986; Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes
. Infect Immun 52:50–55
JacobA. E.,
HobbsS. J.1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol 117:360–372
MurrayB. E.,
SinghK. V.,
HeathJ. D.,
SharmaB. R.,
WeinstockG. M.1990; Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol 28:2059–2063
MurrayB. E.,
SinghK. V.,
RossR. P.,
HeathJ. D.,
DunnyG. M.,
WeinstockG. M.1993; Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements regions encoding biosynthetic function. J Bacteriol 175:5216–5223
QuintilianiR.,
Jr & CourvalinP.1994; Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genetic elements from chromosome to chromosome. FEMS Microbiol Lett 119:359–364
ReimannC.,
HaasD.1993 Mobilization of chromosomes and nonconjugative plasmids by cointegrative mechanisms. . In Bacterial Conjugation , pp. 137–188 . Edited by
ClewellD. B.
New York: Plenum Press;
RossR. P.,
ClaiborneA.1991; Cloning, sequence and overexpression of NADH peroxidase from Streptococcus faecalis 10C1. Structural relationship with the flavoprotein disulfide reductases. J Mol Biol 221:857–871
RossR. P.,
ClaiborneA.1992; Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases. J Mol Biol 227:658–671
SalyersA. A.,
ShoemakerN. B.,
LiL. Y.1995; In the driver’s seat: the Bacteroides conjugative transposons and the elements they mobilize. J Bacteriol 177:5727–5731
StoutV. G.,
IandoloJ. J.1990; Chromosomal gene transfer during conjugation by Staphylococcus aureus is mediated by transposon-facilitated mobilization. J Bacteriol 172:6148–6150
TorresO. R.,
KormanR. Z.,
ZahlerS. A.,
DunnyG. M.1991; The conjugative transposon Tn925: enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis
. Mol Gen Genet 225:395–400
Trieu-CuotP.,
CarlierC.,
CourvalinP.1988; Plasmid transfer by conjugation from Escherichia coli to Gram positive bacteria. FEMS Microbiol Lett 48:289–294
UmedaM.,
OhtsuboE.1989; Mapping of insertion elements IS1, IS2 and IS3 on the Escherichia coli K-12 chromosome. Role of the insertion elements in formation of Hfrs and F’ factors and in rearrangement of bacterial chromosomes. J Mol Biol 208:601–614
YakobsonE. A.,
GuineyD. G.1984; Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5. J Bacteriol 160:451–453