Conjugative transfer of (S) between strains of Enterococcus is associated with the exchange of large fragments of chromosomal DIMA Free

Abstract

Summary: The tetracycline resistance determinant (S) was first detected in antibiotic multiresistant BM4210 and subsequently in strains of Transfer of (S) from clinical isolate BM4242 to strains JH2-2 and OG1RF was found to require the presence in the donor strain of the 55 kb conjugative plasmid pIP825. Comparison of restriction endonuclease generated maps of the donor, the two recipients, and of four transconjugants indicated that transfer of (S) (i) was from chromosome to chromosome, (ii) resulted in the acquisition of an approximately 40 kb element in the same chromosomal region and (iii) was associated with the exchange of large chromosomal fragments. Similar observations were made following conjugal transfer of (S) from four other clinical isolates.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-7-2145
1997-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/7/mic-143-7-2145.html?itemId=/content/journal/micro/10.1099/00221287-143-7-2145&mimeType=html&fmt=ahah

References

  1. Bedzyk L. A., Shoemaker N. B., Young K. E., Salyers A. A. 1992; Insertion and excision of Bacteroides conjugative chromosomal elements. J Bacteriol 174:166–172
    [Google Scholar]
  2. Charpentier E., Gerbaud G., Courvalin P. 1993; Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210. Gene 131:27–34
    [Google Scholar]
  3. Charpentier E., Gerbaud G., Courvalin P. 1994; Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis . Antimicrob Agents Chemother 38:2330–2335
    [Google Scholar]
  4. Courvalin P., Carlier C. 1986; Transposable multiple resistance in Streptococcus pneumoniae . Mol Gen Genet 205:291–297
    [Google Scholar]
  5. Courvalin P., Soussy C. J. 1996; 1996 Report of the Comité de l’Antibiogramme de la Société Française de Microbiologie. Clin Microb Infect 2:S1–S49
    [Google Scholar]
  6. Franke A. E., Clewell D. B. 1981; Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of ‘conjugal’ transfer in the absence of a conjugative plasmid. J Bacteriol 145:494–502
    [Google Scholar]
  7. Franke A. E., Dunny G. M., Brown B. L., An F., Olive D. R., Damle S. P., Clewell D. B. 1978 Gene transfer in Streptococcus faecalis: evidence for the mobilization of chromosomal determinants by transmissible plasmids. . In Microbiology–1978 , pp. 45–47 . Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Gaillard J. L., Berche P., Sansonetti P. 1986; Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes . Infect Immun 52:50–55
    [Google Scholar]
  9. Groisman E. A., Casadaban M. J. 1986; Mini-Mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J Bacteriol 168:357–364
    [Google Scholar]
  10. Grossberger D. 1987; Minipreps of DNA from bacteriophage lambda. Nucleic Acids Res 15:6737
    [Google Scholar]
  11. Jacob A. E., Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol 117:360–372
    [Google Scholar]
  12. Leclercq R., Derlot E., Weber M., Duval J., Courvalin P. 1989; Transferable vancomycin and teicoplanin resistance in Enterococcus faecium . Antimicrob Agents Chemother 33:10–15
    [Google Scholar]
  13. Leclercq R., Dukta-Malen S., Duval J., Courvalin P. 1992; Vancomycin resistance determinant VanC is specific of Enterococcus gallinarum . Antimicrob Agents Chemother 36:10408–10415
    [Google Scholar]
  14. Lloyd R. G., Buchman C. 1995; Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr × F crosses. Genetics 139:1123–1148
    [Google Scholar]
  15. Murray B. E., Singh K. V., Heath J. D., Sharma B. R., Weinstock G. M. 1990; Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol 28:2059–2063
    [Google Scholar]
  16. Murray B. E., Singh K. V., Ross R. P., Heath J. D., Dunny G. M., Weinstock G. M. 1993; Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements regions encoding biosynthetic function. J Bacteriol 175:5216–5223
    [Google Scholar]
  17. Perez-Diaz J. C., Vicente M. F., Baquero F. 1982; Plasmids in Listeria . Plasmid 8:112–118
    [Google Scholar]
  18. Poyart-Salmeron C., Carlier C., Trieu-Cuot P., Courtieu A. L., Courvalin P. 1990; Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes . Lancet 335:1422–1426
    [Google Scholar]
  19. Quintiliani R., Jr & Courvalin P. 1994; Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genetic elements from chromosome to chromosome. FEMS Microbiol Lett 119:359–364
    [Google Scholar]
  20. Reimann C., Haas D. 1993 Mobilization of chromosomes and nonconjugative plasmids by cointegrative mechanisms. . In Bacterial Conjugation , pp. 137–188 . Edited by Clewell D. B. New York: Plenum Press;
    [Google Scholar]
  21. Ross R. P., Claiborne A. 1991; Cloning, sequence and overexpression of NADH peroxidase from Streptococcus faecalis 10C1. Structural relationship with the flavoprotein disulfide reductases. J Mol Biol 221:857–871
    [Google Scholar]
  22. Ross R. P., Claiborne A. 1992; Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases. J Mol Biol 227:658–671
    [Google Scholar]
  23. Salyers A. A., Speer B. S., Shoemaker N. B. 1990; New perspectives in tetracycline resistance. Mol Microbiol 4:151–156
    [Google Scholar]
  24. Salyers A. A., Shoemaker N. B., Li L. Y. 1995; In the driver’s seat: the Bacteroides conjugative transposons and the elements they mobilize. J Bacteriol 177:5727–5731
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Stout V. G., Iandolo J. J. 1990; Chromosomal gene transfer during conjugation by Staphylococcus aureus is mediated by transposon-facilitated mobilization. J Bacteriol 172:6148–6150
    [Google Scholar]
  27. Torres O. R., Korman R. Z., Zahler S. A., Dunny G. M. 1991; The conjugative transposon Tn925: enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis . Mol Gen Genet 225:395–400
    [Google Scholar]
  28. Trieu-Cuot P., Carlier C., Courvalin P. 1988; Plasmid transfer by conjugation from Escherichia coli to Gram positive bacteria. FEMS Microbiol Lett 48:289–294
    [Google Scholar]
  29. Umeda M., Ohtsubo E. 1989; Mapping of insertion elements IS1, IS2 and IS3 on the Escherichia coli K-12 chromosome. Role of the insertion elements in formation of Hfrs and F’ factors and in rearrangement of bacterial chromosomes. J Mol Biol 208:601–614
    [Google Scholar]
  30. Yagi Y., Clewell D. B. 1980; Recombination-deficient mutant of Streptococcus faecalis . J Bacteriol 143:966–970
    [Google Scholar]
  31. Yakobson E. A., Guiney D. G. 1984; Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5. J Bacteriol 160:451–453
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-7-2145
Loading
/content/journal/micro/10.1099/00221287-143-7-2145
Loading

Data & Media loading...

Most cited Most Cited RSS feed