1887

Abstract

Expression studies on the gene encoding aldehyde dehydrogenase in showed induction by two types of molecule (hydroxyaldehydes and 2-oxoglutarate), carbon catabolite repression and respiration dependence. Promoter deletion analysis showed that the proximal operator, which includes inducer-regulator complex and catabolite repression protein (Crp) recognition sites, was necessary for induction by either type of inducer, and that full induction by aldehydes required the cooperation of distal operator sequences beyond position -119. Interactions of the regulator protein with the -59 to -6 fragment were shown by DNA mobility shift assays. Fusions of different deletions of the promoter to indicated that a Crp site proximal to the transcriptional start point (tsp) was functional in the cAMP-dependent catabolite repression of this system, whereas a distal control site was likely to operate in a cAMP-independent catabolite repression. DNA mobility shift and footprint analyses showed that only the tsp proximal site was bound by pure Crp with a of 5.4 x 10 M. As shown by an Arc-defective strain, the gene seems to be repressed by the Arc system under anaerobiosis, displaying its physiological full induction and activity in the presence of oxygen.

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1997-06-01
2021-08-03
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References

  1. Adhya S., Gottesman M., Garges S., Oppenheim A. 1993; Promoter resurrection by activators – a minireview.. Gene 132:1–6
    [Google Scholar]
  2. Baldomà L., Aguilar J. 1987; Involvement of lactaldehyde dehydrogenase in several metabolic pathways of Escherichia coli K-12.. J Biol Chem 262:13991–13996
    [Google Scholar]
  3. Baldomà L., Aguilar J. 1988; Metabolism of l-fucose and l-rhamnose in Escherichia coli: aerobic regulation of l-lactaldehyde dissimilation.. J Bacteriol 170:416–421
    [Google Scholar]
  4. Barber A. M., Zhurkin V. B., Adhya S. 1993; CRP-binding sites: evidence for two structural classes with 6-bp and 8-bp spacers.. Gene 130:1–8
    [Google Scholar]
  5. Belasco J. G., Beatty T., Adams C. W., von Gabain A., Cohen S. N. 1985; Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript.. Cell 40:171–181
    [Google Scholar]
  6. Boronat A., Aguilar J. 1979; Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme.. J Bacteriol 140:320–326
    [Google Scholar]
  7. Boronat A., Aguilar J. 1981; Metabolism of l-fucose and l-rhamnose in Escherichia coli: differences in induction of propanediol oxidoreductase.. J Bacteriol 147:181–185
    [Google Scholar]
  8. Boronat A., Caballero E., Aguilar J. 1983; Experimental evolution of a metabolic pathway for ethylene glycol utilization by Escherichia coli.. J Bacteriol 153:134–139
    [Google Scholar]
  9. Caballero E., Baldomà L., Ros J., Boronat A., Aguilar J. 1983; Identification of lactaldehyde dehydrogenase and glycolaldehyde dehydrogenase as functions of the same protein in Escherichia coli.. J Biol Chem 258:7788–7792
    [Google Scholar]
  10. Chan B., Spassky A., Busby S. 1990; The organization of open complexes between Escherichia coli RNA polymerase and DNA fragments carrying promoters either with or without consensus — 35 region sequences.. Biochem J 270:141–148
    [Google Scholar]
  11. Chen Y. M., Zhu Y., Lin E. C. C. 1987; NAD-linked aldehyde dehydrogenase for aerobic utilization of l-fucose and l-rhamnose by Escherichia coli.. J Bacteriol 169:3289–3294
    [Google Scholar]
  12. Cocks G. T., Aguilar J., Lin E. C. C. 1974; Evolution of the 1,2-propanediol catabolism in Escherichia coli by recruitment of enzymes for l-fucose and l-lactate metabolism.. J Bacteriol 118:83–88
    [Google Scholar]
  13. de Crombrugghe B., Busby S., Buc H. 1984; Cyclic AMP receptor protein: role in transcription activation.. Science 224:831–838
    [Google Scholar]
  14. Friedman D. I. 1988; Integration host factor: a protein for all reasons.. Cell 55:545–554
    [Google Scholar]
  15. Granston B. E., Nash H. A. 1993; Characterization of a set of integration host factor mutants deficient for DNA binding.. J Mol Biol 234:45–59
    [Google Scholar]
  16. Hidalgo E., Demple B. 1994; An iron-sulfur center essential for transcriptional activation by redox-sensing SoxR protein.. EMBO J 13:138–146
    [Google Scholar]
  17. Hidalgo E., Chen Y. M., Lin E. C. C., Aguilar J. 1991; Molecular cloning and DNA sequencing of the gene aid encoding lactaldehyde dehydrogenase from Escherichia coli.. J Bacteriol 173:6118–6123
    [Google Scholar]
  18. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids.. Anal Biochem 114:193–197
    [Google Scholar]
  19. Iuchi S., Lin E. C. C. 1988; ArcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in anaerobic pathways.. Proc Natl Acad Sci USA 85:1888–1892
    [Google Scholar]
  20. Iuchi S., Lin E. C. C. 1992; Mutational analysis of signal transduction by ArcB, a membrane sensor protein responsible for anaerobic repression of operons involved in the central aerobic pathways of Escherichia coli.. J Bacteriol 174:3972–3980
    [Google Scholar]
  21. Kolb A., Busby S., Buc H., Garges S., Adhya S. 1993; Transcriptional regulation by cAMP and its receptor protein.. Annu Rev Biochem 62:749–795
    [Google Scholar]
  22. Kumar A., Grimes B., Fugita N., Makino R., Malloch A., Hayward R. S., Ishihama A. 1994; Role of the σ70 subunit of Escherichia coli RNA-polymerase in transcription activation.. J Mol Biol 235:405–113
    [Google Scholar]
  23. Lavigne M., Herbert M., Kolb A., Buc H. 1992; Upstream curved sequences influence the initiation of transcription at the Escherichia coli galactose operon.. J Mol Biol 224:293–306
    [Google Scholar]
  24. LeBlanc D., Mortlock R. P. 1971; Metabolism of D-arabinose: a new pathway in Escherichia coli.. J Bacteriol 106:90–96
    [Google Scholar]
  25. lin E. C. C. 1976; Glycerol dissimilation and its regulation in bacteria.. Annu Rev Microbiol 80:535–578
    [Google Scholar]
  26. Lynch A. S., Lin E. C. C. 1996; Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli:characterization of DNA binding at target promoters.. J Bacteriol 178:6238–6249
    [Google Scholar]
  27. Meissner P. S., Sisk W. P., Berman M. L. 1987; Bacteriophage λ cloning system for the construction of directional cDNA libraries.. Proc Natl Acad Sci USA 84:4171–4178
    [Google Scholar]
  28. Miller J. H. 1992; A Short Course in Bacterial Genetics.Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  29. Moralejo P., Egan S. M., Hidalgo E., Aguilar J. 1993; Sequencing and characterization of a gene cluster encoding the enzymes for l-rhamnose metabolism in Escherichia coli.. J Bacteriol 175:5585–5594
    [Google Scholar]
  30. Mozola M. A., Friedman D. I. 1985; A phi 80 function inhibitory for growth of lambdoid phage in himmutants of Escherichia coli deficient in IHF. Genetic analysis of the Rha phenotype.. J Virol 140:313–327
    [Google Scholar]
  31. Nash H. A., Granston A. E. 1991; Similarity between the DNA binding domains of IHF protein and TFIID protein.. Cell 67:1037–1038
    [Google Scholar]
  32. Nunoshiba T., Hidalgo E., Amabile-Cuevas C. F., Demple B. 1992; Two stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene.. J Bacteriol 174:6054–6060
    [Google Scholar]
  33. Quintillá F. X., Baldomci L., Badfa J., Aguilar J. 1991; Aldehyde dehydrogenase induction by glutamate in Escherichia coli. Role of 2-oxoglutarate.. Eur J Biochem 202:1321–1325
    [Google Scholar]
  34. Roth J. 1970; Genetic techniques in studies of bacterial metabolism.. Methods Enzymol 17:1–35
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  37. Schmid M. B. 1990; More than just ‘histone-like’ proteins.. Cell 63:451–153
    [Google Scholar]
  38. Simons R. W., Houman F., Kleckner N. 1987; Improved single and multicopy lac-based cloning vectors for protein and operon fusions.. Gene 53:85–96
    [Google Scholar]
  39. Sridhara S., Wu T. T. 1969; Purification and properties of lactaldehyde dehydrogenase from Escherichia coli.. J Biol Chem 244:5233–5238
    [Google Scholar]
  40. Summers A. O. 1992; Untwist and shout: a heavy metal-responsive transcriptional regulator.. J Bacterial 174:3097–3101
    [Google Scholar]
  41. Ullmann A., Tillier F., Monod J. 1976; Catabolite modulator factor: a possible mediator of catabolite repression in bacteria.. Proc Natl Acad Set USA 73:3476–3479
    [Google Scholar]
  42. Wu Y., Datta P. 1992; Integration host factor is required for positive regulation of the tdc operon of Escherichia coli.. J Bacteriol 174:233–240
    [Google Scholar]
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