1887

Abstract

M5 and DL1 each express two related adhesin polypeptides, SspA and SspB, which are members of the antigen I/II family of streptococcal surface proteins. The and genes are tandemly arranged in both strains, with residing upstream of The genes are separated by approximately 400 nucleotides in DL1 and 1300 nucleotides in M5. The nucleotide sequence of the intergenic region of strain M5 is reported and the difference in length compared to DL1 shown to arise from the presence of an insertion sequence, designated IS consisting of 1197 bp. The nucleotide sequence of IS is highly homologous to IS of and is related to a lesser extent to other members of the IS family of bacterial insertion sequences. It contains a single ORF of 1026 bp, encoding a putative transposase polypeptide of 342 amino acids. The deduced transposase sequence exhibits 93% identity with the transposase polypeptides encoded by IS However, the protein lacks a 90 residue central domain that is present in the IS transposase and in the transposase polypeptides encoded by the related IS elements. In addition, the organization of the inverted repeats flanking the transposase gene in differs from IS Extension products generated from a -specific primer indicated that transcription initiates within the intergenic region in both strains, suggesting that and are independently transcribed. Transcription appears to initiate 42 bases upstream of in DL1 In contrast, transcription in M5 initiates at least 125 bases upstream of in close proximity to the terminal inverted repeat of IS These results indicate that the promoters of M5 and DL1 are not conserved and suggest that IS sequences may play a role in directing the expression of in M5.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-6-2047
1997-06-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/6/mic-143-6-2047.html?itemId=/content/journal/micro/10.1099/00221287-143-6-2047&mimeType=html&fmt=ahah

References

  1. Andersen R. N., Ganeshkumar N., Kolenbrander P. E. 1993; Cloning of the Streptococcus gordonii PK488 gene, encoding an adhesin which mediates coaggregation with Actinomyces naeslundii PK606.. Infect Immun 61:981–987
    [Google Scholar]
  2. Bennett P. M. 1991; Transposable elements and transposition in bacteria. In Modern Microbial Genetics, pp. Edited by U. N. Streips & R. E. Yasbin. New York: Wiley-Liss.. 324–364
    [Google Scholar]
  3. Byrne M. E., Gillespie M. T., Skurray R. A. 1991; 4′,4″ adenyltransferase activity on conjugative plasmids isolated from Staphylococcus aureus is encoded on an integrated copy of pUB110.. Plasmid 25:70–75
    [Google Scholar]
  4. Chandler M., Fayet O. 1993; Translational frameshifting in the control of transposition in bacteria.. Mol Microbiol 7:497–503
    [Google Scholar]
  5. Cirillo J. D., Baretta R., Bloom B. R., Jacobs W. R. 1991; A novel transposon trap for mycobacteria: isolation and characterization of IS1096.. J Bacteriol 173:7772–7780
    [Google Scholar]
  6. Demuth D. R., Davis C. A., Corner A. M., Lamont R. J., Leboy P. S., Malamud D. 1988; Cloning and expression of a Streptococcus sanguis surface antigen that interacts with a human salivary agglutinin.. Infect Immun 56:2484–2490
    [Google Scholar]
  7. Demuth D. R., Berthold P., Leboy P. S., Golub E. E., Davis C. A., Malamud D. 1989; Saliva-mediated aggregation of Enterococcus transformed with a Streptococcus sanguis gene encoding the Ssp-5 surface antigen.. Infect Immun 57:1470–1475
    [Google Scholar]
  8. Demuth D. R., Golub E. E., Malamud D. 1990a; Streptococcal-host interactions: structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein.. J Biol Chem 265:7120–7126
    [Google Scholar]
  9. Demuth D. R., Lammey M. S., Huck M., Lally E. T., Malamud D. 1990b; Comparison of Streptococcus mutans and Streptococcus sanguis receptors for human salivary agglutinin.. Microb Pathog 9:199–211
    [Google Scholar]
  10. Demuth D. R., Duan Y., Brooks W., Holmes A. R., McNab R., Jenkinson H. F. 1996; Tandem genes encode cell-surface polypeptides SspA and SspB which mediate adhesion of the oral bacterium Streptococcus gordonii to human and bacterial receptors.. Mol Microbiol 20:403–413
    [Google Scholar]
  11. Derbise A., Dyke K. G. H., El Solh N. 1994; Isolation and characterization of IS1181, an insertion sequence from Staphylococcus aureus.. Plasmid 31:251–264
    [Google Scholar]
  12. Derbise A., Dyke K. G. H., El Solh N. 1995; Rearrangements in the staphylococcal beta-lactamase-encoding plasmid, pIP1066, including a DNA inversion that generates two alternative transposons.. Mol Microbiol 17:769–779
    [Google Scholar]
  13. DeShazer D., Wood G. E., Friedman R. L. 1994; Molecular characterization of catalase from Bordeteffa pertussis: identitication of the katA promoter in an upstream insertion sequence.. Mol Microbiol 14:123–130
    [Google Scholar]
  14. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Res 12:387–395
    [Google Scholar]
  15. Dodd H. M., Horn N., Gasson M. J. 1994; Characterization of IS905, a new multicopy insertion sequence identified in lactococci.. J Bacteriol 176:3393–3396
    [Google Scholar]
  16. Frandsen E. V. G., Pedrazzoli V., Kilian M. 1991; Ecology of viridans streptococci in the oral cavity and pharynx.. Oral Microbiol Immunol 6:129–133
    [Google Scholar]
  17. Galas D. J., Chandler M. 1989; Bacterial insertion sequences. In Mobile DNA, pp. Edited by D. E. Berg & M. M. Howe. Washington, DC: American Society for Microbiology.. 109–162
    [Google Scholar]
  18. Gibbons R. J., Hay D. I., Schlesinger D. H. 1991; Delineation of a segment of absorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces.. Infect Immun 59:2948–2954
    [Google Scholar]
  19. Hanahan D. 1983; Studies on the transformation of Escherichia coli with plasmids.. J Mol Biol 166:557–580
    [Google Scholar]
  20. Handwerger S., Skoble J., Discotto L. F., Pucci M. J. 1995; Heterogeneity of the vanA gene cluster in clinical isolates of enterococci from the northeastern United States.. Antimicrob Agents Chemother 39:362–368
    [Google Scholar]
  21. Hasty D. L., Ofek I., Courtney H. S., Doyle R. J. 1992; Multiple adhesins of streptococci.. Infect Immun 60:2147–2152
    [Google Scholar]
  22. Jenkinson H. F., Easingwood R. A. 1990; Insertional inactivation of a gene encoding a 76 kilodalton cell surface polypeptide in Streptococcus gordonii Challis has a pleiotropic effect on cell surface composition and properties.. Infect Immun 58:3689–3697
    [Google Scholar]
  23. Jenkinson H. F., Terry S. D., McNab S. D., Tannock G. W. 1993; Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces.. Infect Immun 61:3199–3208
    [Google Scholar]
  24. Johansen E., Kibenich A. 1992; Isolation and characterization of IS1165, an insertion sequence of Leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria.. Plasmid 27:200–206
    [Google Scholar]
  25. Kelly C., Evans P., Bergmeier L., Lee S. F., Progulske-Fox A., Harris A. C., Aitken A., Bleiweis A. S., Lehner T. 1989; Sequence analysis of the cloned streptococcal cell surface antigen I/II.. FEBS Lett 258:127–132
    [Google Scholar]
  26. Kolenbrander P. E., London J. 1993; Adhere today, here tomorrow: oral bacterial adherence.. J Bacteriol 175:3247–3252
    [Google Scholar]
  27. Lamont R. J., Gil S., Demuth D. R., Malamud D., Rosan B. 1994; Molecules of Streptococcus gordonii that bind to Porphyromonas gingivalis.. Microbiology 140:867–872
    [Google Scholar]
  28. LaPolla R. J., Haron J. A., Kelly C. G., Taylor W. R., Bohart C., Hendricks M., Pyati J., Graff R. T., Ma J. K.-C., Lehner T. 1991; Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus.. Infect Immun 59:2677–2685
    [Google Scholar]
  29. Ma J. K.-C., Kelly C. G., Munro G., Whiley R. A., Lehner T. 1991; Conservation of the gene encoding antigen I/II in oral streptococci.. Infect Immun 59:2686–2694
    [Google Scholar]
  30. McNab R., Jenkinson H. F. 1992; Gene disruption identifies a 290 kDa cell-surface polypeptide conferring hydrophobicity and coaggregation properties in Streptococcus gordonii.. Mol Microbiol 6:2939–2949
    [Google Scholar]
  31. Matthews P. R., Stewart P. R. 1988; Amplification of a section of chromosomal DNA in methicillin-resistant Staphylococcus aureus following growth in high concentrations of methicillin.. J Gen Microbiol 134:1455–1464
    [Google Scholar]
  32. Nyvad B., Kilian M. 1990; Comparison of the initial streptococcal microflora on dental enamel in caries-active and caries-inactive individuals.. Caries Res 24:267–272
    [Google Scholar]
  33. Platt T. 1986; Transcriptional termination and the regulation of gene expression.. Annu Rev Biochem 55:339–372
    [Google Scholar]
  34. Podglajen I., Brueil J., Collatz E. 1994; Insertion of a novel DNA sequence, IS1186, upstream of the silent carbapenemase gene cfiA, promotes expression of carbapenem resistance in clinical isolates of Bacteroides fragilis.. Mol Microbiol 12:105–114
    [Google Scholar]
  35. Reddy K. J., Webb R., Sherman L. A. 1990; Bacterial RNA isolation with a one hour centrifugation in a table top ultra-centrifuge.. Bio Techniques 8:250–251
    [Google Scholar]
  36. Sabelnikov A. G., Greenberg B., Lacks S. A. 1995; An extended −10 promoter alone directs transcription of the DpnII operon of Streptococcus pneurnoniae.. J Mol Biol 250:144–155
    [Google Scholar]
  37. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.. 2:
    [Google Scholar]
  38. Scannapieco F. A., Bergey E. J., Reddy M. S., Levine M. J. 1989; Characterization of salivary α-amylase binding to Streptococcus sanguis.. Infect Immun 57:2853–2863
    [Google Scholar]
  39. Wada A., Katayama Y., Hiramatsu K., Yokota T. 1991; Southern hybridization analysis on the mecA deletion from methicillin resistant Staphylococcus aureus.. Biochem Biophys Res Commun 176:1319–1325
    [Google Scholar]
  40. van der Zee A., Agterberg C., van Agterveld M., Peeters M., Mooi F. R. 1993; Characterization of ISl001, an insertion sequence element of Bordetella parapertussis.. J Bacteriol 175:141–147
    [Google Scholar]
  41. Zhou L., Morrison D. A. 1995; The new insertion sequence IS1167 of Streptococcus pneumoniae is related to IS1096 and a family of IS elements occurring widely among Gram positive bacteria.. Dev Biol Stand 85:77–81
    [Google Scholar]
  42. Zhou L., Hui F. M., Morrison D. A. 1995; Characterization of IS1167, a new insertion sequence in Streptococcus pneumoniae.. Plasmid 33:127–138
    [Google Scholar]
/content/journal/micro/10.1099/00221287-143-6-2047
Loading
/content/journal/micro/10.1099/00221287-143-6-2047
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error