1887

Abstract

The gene of the budding yeast is required for the arrest of cell proliferation upon nutrient exhaustion: mutants carry on dividing and in the absence of growth become abnormally l. It is reported here that overexpression of Whi2 from the promoter results in filamentous growth - cells fail to complete cytokinesis, the budding pattern changes from axial to polar, cells become elongated and cell size increases threefold. In many ways, these filaments resemble the pseudohyphae which result from nitrogen-limited growth and the filaments seen during the invasive growth of haploids. However, Whi2-induced filament formation is reduced, but not blocked, by mutations in or which do block pseudohypha formation. Furthermore, pseudohypha formation can still occur in a diploid in which both copies of the gene have been deleted. Thus Whi2-induced filament formation and pseudohypha formation must come about through the action of different pathways. Despite this, a mutation in the gene, which is required for pseudohypha formation, does block Whi2-induced filament formation. Concanavalin A pulse-chase experiments show that new cell wall material is incorporated only into the tips of the apical cells. An extragenic suppressor of the allele also results in filamentous growth. These results suggest that Whi2 negatively regulates a function required for the budding mode of cell proliferation including cytokinesis. This function is defined wholly or in part by the allele.

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1997-06-01
2021-10-20
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References

  1. Adams A. E. M., Pringle J. R. 1983; Relationship of actin and tubulin distribution to bud growth in wild type and morphogenetic mutant Saccharomyces cerevisiae.. J Cell Biol 98:934–945
    [Google Scholar]
  2. Bender A., Pringle J. R. 1989; Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and 3 newly identified gcnes including the ras-related gene RSR1. . Proc Natl Acad Sci USA 86:9976–9980
    [Google Scholar]
  3. Chant J., Herskowitz I. 1991; Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway.. Cell 65:1203–1212
    [Google Scholar]
  4. Chant J., Pringle J. R. 1995; Patterns of bud-site selection in the yeast Saccharomyces cerevisiae. . J Cell Biol 129:751–765
    [Google Scholar]
  5. Chant J., Corrado K., Pringle J. R., Herskowitz I. 1991; Yeast BUD5, encoding a putative GDP-GTP exchange factor, is nccessary for bud site selection and interacts with bud formation gene BEMI.. Cell 65:1213–1224
    [Google Scholar]
  6. Cvrckova F., Devirgilio C., Manser E., Pringle J. R., Nasmyth K. 1995; Ste.20-like protein-kinases are required for normal localization of cell-growth and for cytokinesis in budding yeast.. Genes Dev 9:1817–1830
    [Google Scholar]
  7. Elliott B., Futcher B. 1993; Stress resistance of yeast cells is largely independent of cell cycle phase.. Yeast 9:33–42
    [Google Scholar]
  8. Freifelder D. 1960; Bud position in Saccharomyces cerevisiae.. J BLzcteriol 80:567–568
    [Google Scholar]
  9. Gimeno C. J., Fink G. R. 1992; The logic of cell1 division in the life cycle of yeast.. Science 257:626
    [Google Scholar]
  10. Girneno C. J., Ljungdahl P. O., Styles C. A., Fink G. R. 1992; Unipolar cell divisions in the yeast Saccharomyces cerevisiae lead to filamentous growth : regulation by starvation and RAS.. Cell 68:1077–1090
    [Google Scholar]
  11. Halme A., Michelitch M., Mitchell E. L., Chant J. 1996; BudlOp directs axial cell polarisation in budding yeast and rcsembles a transmembrane receptor.. Curr Biol 6:570–579
    [Google Scholar]
  12. Hartwell L. H. 1974; The Saccharomyces cell cycle.. Bacterial Rev 38:164–198
    [Google Scholar]
  13. Johnston G. C., Pringle J. R., Hartwell L. H. 1977; Coordination of growth with cell division in the yeast Saccharomyces ccrevisiae.. Exp Cell Res 105:79–98
    [Google Scholar]
  14. Kelly D. E., Trevethick J., Mountain H., Sudbery P. E. 1988; l-ranscript characterization, gene disruption and nucleotide sequence of the Saccharomyces cerevisiae WHIZ gene.. Gene 66:205–213
    [Google Scholar]
  15. Kurjan J. 1993; The pheromone response pathway in Saccharonzyces cerevisiae.. Annu Rev Genet 27:147–179
    [Google Scholar]
  16. Liu H., Styles C. A., Fink G. R. 1993; Elements of the yeast pheromone response pathway required for filamentous growth of diploids.. Science 262:1741–1744
    [Google Scholar]
  17. Manser E., Leung T., Salihuddin H., Zhao Z. S., Lim L. 1994; A brain serine specific protein kinase activated by Cdc42 and Rac1.. Nature 367:40–46
    [Google Scholar]
  18. Mountain H. A., Sudbery P. E. 1990a; Regulation of the Saccharomyces cerevisiae WH12 gene.. J Gen Microbiol 136:727–732
    [Google Scholar]
  19. Mountain H. A., Sudbery P. E. 1990b; The relationship of growth rate and catabolite repression with WH12 expression and cell size in .Saccharomyces cerevisiae.. J Gen Microbiol 136:733–737
    [Google Scholar]
  20. Pringle J. R., Hartwell L. H. 1981; The Saccharomyces cerevisiae cell cycle. Edited by J. N. Strathern, E. W. Jones & J. R. Broach. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. In The Molecular Biology of the Yeast Saccharomyces : Life Cycle and Inheritance pp.:97–142
    [Google Scholar]
  21. Pringle J. R., Adams A. E. M., Drubin D. G., Haarer B. K. 1991; Immunofluorescence methods for yeast. Edited by C. Guthrie & G. R. Fink. San Diego, CA: Academic Press.. In Guide t o Yeast Genetics and Molecular Biology pp:565–601
    [Google Scholar]
  22. Radcliffe P. A., Binley K. M., Trevethick J., Sudbery P. E. 1997; Deregulation of G1 cyclins in the Saccharornyces cerevisiae WH12 mutant. Yeast (in press)..
    [Google Scholar]
  23. Rahman D. R. J., Sudbery P. E., Kelly S., Marison I. W. 1988; The effect of dissolved oxygen concentration on the growth physiology of Saccharomyces cerevisiae whi2 mutants.. J Gen Microbiol 134:2241–2248
    [Google Scholar]
  24. Reisdorf P., Maarse A. C., Daignan-Fournier B. 1993; Epitope tagging vectors for yeast.. Curr Genet 23:181–183
    [Google Scholar]
  25. Roberts R. L., Fink G. R. 1994; Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the SmaIl cell type: mating and invasive growth.. Genes Dev 8:2974–2985
    [Google Scholar]
  26. Sanders S.L., Field C. M. 1994; Cell division: septins in common?. Curr Biol 4:907–910
    [Google Scholar]
  27. Saul D. J., Sudbery P. E. 1985; Molecular-cloning of whi2, a gene involved in the regulation of cell-proliferation in Saccharomyces cerevisiae. . J Gen Microbiol 131:1797–1806
    [Google Scholar]
  28. Saul D. J., Walton E. F., Sudbery P. E., Carter B. L. A. 1985; Saccharomyces cerevisiae whi2 mutants in stationary phase retain the properties of exponentially growing cells.. J Gen Microbiol 131:2245–2251
    [Google Scholar]
  29. Snyder M. 1989; The Spa2 protein of yeast localizes to sites of cell-growth.. J Cell Biol 108:1419–1429
    [Google Scholar]
  30. Sudbery P. E., Goodey A. R., Carter B. L. C. 1980; Genes that control cell proliferation in the yeast Saccharomyces cerevisiae. . Nature 288:401–404
    [Google Scholar]
  31. Werner-Washburne M., Braun E., Johnston G. C., Singer R. A. 1993; Stationary phase in the yeast Saccharomyces cerevisiae. . Microbiol Rev 57:383–401
    [Google Scholar]
  32. Wright R. M., Repine T., Repine J. E. 1993; Pseudohyphal growth in haploid Saccharomyces cerevisiae is a reversible process.. Curr Genet 23:388–391
    [Google Scholar]
  33. Zahner J. E., Harkins H. A., Pringle J. R. 1996; Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae.. Mol Cell Biol 16:1857–1870
    [Google Scholar]
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