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Volume 143, Issue 5

Analysis of a new dimeric extradiol dioxygenase from a naphthalenesulfonate-degrading sphingomonad Free

Abstract

A new extradiol dioxygenase was cloned by screening a gene bank from the naphthalenesulfonate-degrading bacterial strain BN6 for colonies with 2,3-dihydroxybiphenyl dioxygenase (DHBPDO) activity. A 16 kb DNA fragment was sequenced and an ORF of 954 bp identified. Comparison of the deduced amino acid sequence of DHBPDO II from strain BN6 with previously published sequences showed the closest relationship to a metapyrocatechase (Mpcll) from JMP 222. Thus, the enzyme was only distantly related to the main groups of catechol 2,3-dioxygenases or DHBPDOs. The dioxygenase was expressed using a T7 expression vector and the enzymic characteristics of the protein were examined. The enzyme oxidized 2,3-dihydroxybiphenyl, 3-isopropylcatechol, 3-methylcatechol, 4-fluorocatechol and 1,2-dihydroxynaphthalene. Comparison of the UV/visible spectrum of the product formed from 3,5-dichlorocatechol with previous reports suggested that this substrate is oxidized by different extradiol dioxygenases either by proximal or distal ring cleavage. The enzyme required Fefor maximal activity. In contrast to most other extradiol dioxygenases, the enzyme consisted of only two identical subunits.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 2,3-dihydroxybiphenyl dioxygenase , extradiol dioxygenases , Sphingomonas and strain BN6
© Society Society for General Microbiology 1997
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1997-05-01
2024-04-25
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Analysis of a new dimeric extradiol dioxygenase from a naphthalenesulfonate-degrading sphingomonad
Microbiology 143, 1691 (1997); https://doi.org/10.1099/00221287-143-5-1691
/content/journal/micro/10.1099/00221287-143-5-1691
/content/journal/micro/10.1099/00221287-143-5-1691
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