Wild-type cells of the pathogenic black yeast grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-β-glucan antiserum, indicating that they are linked to 1,6-β-glucan. It is proposed that 1,6-β-glucosylated cell wall proteins generally occur among ascomycetes.


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