A protopectinase (PPase)-encoding gene, from was cloned by colony hybridization using two oligonucleotide probes synthesized from the N-terminal amino acid sequences of native PPase SE1 and one peptide from a lysyl endopeptidase digest. Nucleotide sequencing revealed that contains an ORF encoding a 367 amino acid protein. Mature PPase SE3 is composed of 340 amino acids and the N-terminus of the ORF appeared to correspond to a signal peptide and a propeptide processed by a KEX2-like proteinase. The deduced amino acid sequence of was 65.4, 56.7, 58.1, 61.8 and 48.9% homologous to the polygalacturonases of and respectively. One domain, which might interact with polygalacturonic acid, is highly conserved not only in fungal polygalacturonases but also in bacterial and plant polygalacturonases. was expressed in but three forms (the mature form, a glycosylated form and an uncharacterized processed form) of PPase SE3 were present among the products.


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