1887

Abstract

A kinetic method to measure the intracellular concentration of respiratory substrates in short-term starvation-enrichment experiments is proposed. Samples of bacterial suspension from steady-state chemostat cultures were subjected to 25 min starvation, followed by pulse addition of [C]glucose. Residual substrate utilization rates and respiration rates (uptake of dissolved O) before and after amendment were recorded. Increases in pool sizes (δ) during transients were calculated on the basis of C balance. The dependence of respiration rate q on δ was found to obey modified Michaelis-Menten kinetics: = (LC+δ)/( +LC+δ) [ is maximal respiration rate (29.1 mmol O hper g biomass C), = 12.14 mg C per g biomass C], where LC is the absolute value of the pool size before amendment. Direct chemical determination of LC in cold TCA extracts revealed two fractions. The first fraction was mobile and showed a close correlation with both respiration and The second, ‘stable’, fraction did not correlate with respiration dynamics and was interpreted as material formed artifactually by acid degradation of polymeric cell components.

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1997-05-01
2024-04-20
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References

  1. Domach M. M., Leung S. K., Cahn R. E., Cacks G. G., Shuler M. L. 1984; Computer model for glucose-limited growth of single cell of Escherichia coli B/r-A. . Biotechnol Bioeng 26:203–206
    [Google Scholar]
  2. Dorofeyev A. G., Panikov N. S. 1992; A quantitative description of microorganism growth in a batch culture depending on the physiologial state of the inoculate. Microbiology (English translation of Mikrobiologiya) 60:448–455
    [Google Scholar]
  3. Fredrickson A. G. 1976; Formulation of structured growth models. Biotechnol Bioeng 18:1481–1486
    [Google Scholar]
  4. Guthke R., Knorre W. A. 1980; Oscillatory and multistability behavior in continuous culture models : the S-P-X model. Proceedings of the 7th Symposium, Prague, 10-14 July 1978, pp. Edited by B. Sikyta, 2. Fencl & V. Polacek. Prague: Czechoslovak Academy of Sciences. In Continuous Cultivation of Micro-organisms pp:207–214
    [Google Scholar]
  5. Herbert D. 1958; Some principles of continuous culture. In Edited by G. Tunevall. Stockholm : Almquist & Wiksell. Recent Progress in Microbiology (VII International Congress for Microbiology) pp:381–396
    [Google Scholar]
  6. lerusalimsky N. D. 1967; , Bottle-necks in metabolism as growth rate controlling factors. Edited by E. 0. Powell, C. G. T. Evans, R. E. Strange & D. W. Tempest. Salisbury: HMSO.. In Microbial Physiology and Continuous Culture pp:23–33
    [Google Scholar]
  7. Jeong J. W., Snay J., Ataai M. M. 1990; A mathematical model for examining growth and sporulation processes of Bacillus subtilis. . Biotechnol Bioeng 35:160–184
    [Google Scholar]
  8. Krutko P. D., Maximov A. I., Skwortzov L. M. 1988; Algorithms and Computer Programs in Designing of Automatic Systems. Moscow : Radyo y Svyaz (in Russian)..
    [Google Scholar]
  9. Monod J. 1942; Recherches sur la Croissance des Cultures Bacthiennes. Paris : Hermann..
    [Google Scholar]
  10. Monod J. 1950; La technique de culture continue: thkorie et applications. Ann Znst Pasteur 79:390–410
    [Google Scholar]
  11. Nyholm N. 1976; A mathematical model for microbial growth under limitation by conservative substrates. Biotechnol Bioeng 18:1043–1056
    [Google Scholar]
  12. Panikov N. S. 1991; A synthetic chemostat model as a tool for description of complex dynamic behavior of microorganisms. 43141. Microbiology (English translation of Mikrobiologiya) 60:431–441
    [Google Scholar]
  13. Pannikov N. S. 1995; Microbial Growth Kinetics. London & Glasgow: Chapman & Hall..
    [Google Scholar]
  14. Panikov N. S., Shekhovtsova N. V., Dorofeyev A. G., Zvyagintsev D. G. 1989; Quantitative investigation of the dynamics of death in starving microorganisms. Microbiology (English translation of Mikrobiologiya) 57:786–794
    [Google Scholar]
  15. Pirt S. J. 1975; Principles of Microbe and Cell Cultivation. Oxford : Blackwell Scientific Publications..
    [Google Scholar]
  16. Pirt W. M., Pirt S. J. 1977; Photosynthetic production of biomass and starch by Chlorella in chemostat culture. J Appl Chem Biotechnol 27:643–650
    [Google Scholar]
  17. Powell E. O. 1967; The growth rate of microorganisms as a function of substrate concentration. Edited by E. 0. Powell, C . G. T. Evans, R. E. Strange & D. W. Tempest. Salisbury: HMSO. In Microbial Physiology and Continuous Culture pp. 34–56
    [Google Scholar]
  18. Powell E. O. 1969; Transient changes in the growth rate of micoorganisms. Edited by I. Malek, K. Beran, Z. Fencl, V. Munk, J. Ricica & H. Smrckova. New York: Academic Press. In Continuous Cultivation of Microorganisms pp:275–284
    [Google Scholar]
  19. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol 64:171–185
    [Google Scholar]
  20. Varfolomeyev S . D. 1987; The kinetic principles of microbial populations development. Moscow : Moscow University Publications (in Russian). In Modern Problems of Biokinetics pp:6–77
    [Google Scholar]
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