A restriction map of the 8 Mb linear chromosome of R6-501 was constructed for the enzymes (13 fragments) and (7 fragments). Linking clones for all 12 sites and 5 of the 6 sites were isolated. The chromosome has terminal inverted repeats of 550 kb, which are the longest yet reported for a species. The oxytetracycline gene cluster lies about 600 kb from one end, which might account for its frequent spontaneous amplification and deletion. Several other markers were localized on the chromosome ( and the operons, the attachment site for pSAM2 and prophages RP2 and RP3). Comparison of the conserved markers with the map of A3(2) suggested there are differences in genome organization between the two species.


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