1887

Abstract

As part of a larger study investigating diversity and distribution of spp. in Australia, multilocus enzyme electrophoresis was used to assess genetic relationships at 17 enzyme loci amongst a collection of reference strains and isolates initially identified on biochemical and other grounds as (70), ‘X’ mycobacteria (10), (7), (8) and subsp. (2). Two of the isolates initially identified as were shown to be quite distinct from the others. Both gave negative results in a species-specific DNA probe test, whilst one was positive by PCR. These results emphasize the uncertainties involved in identifying members of this group. The other isolates formed a cohesive but diverse group, being divided into 48 electrophoretic types (ETs), with a mean genetic diversity of 0∙38. Forty-three of these ETs contained only single isolates. There was no clear relationship between the serovar and ET designation. The index of association calculated for was significantly different from zero, suggesting that it is a clonal species. PFGE was also applied to selected isolates from the ETs containing multiple isolates, and some of these could be differentiated further. The strains of and ‘X’ mycobacteria were distinct from , but themselves were highly heterogeneous, with mean genetic diversities of 0∙66 and 0∙65, respectively. Each of these groups may represent more than one species. strains were distinct from the two subsp. strains, as well as from the other mycobacteria studied.

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1997-04-01
2021-08-02
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References

  1. Arbeit R. D., Slutsky A., Barber T. W., Maslow J. N., Niemczyk S., Falkinham J. O., O’Connor G. T., Von Reyn C. F. 1993; Genetic diversity among strains of Mycobacterium avium causing monoclonal and polyclonal bacteremia in patients with AIDS.. J Infect Dis 167:1348–1390
    [Google Scholar]
  2. Brooks R. W., Parker B. C., Graft H., Falkinham J. O. 1984; Epidemiology of infection with non-tuberculous mycobacteria. V. Numbers in the eastern United States soils and correlation with soil characteristics.. Am Rev Respir Dis 130:630–633
    [Google Scholar]
  3. Burr E. J. 1970; Division sorting with mixed character types. 11. Fusion strategies.. Aust Comp J 2:98–103
    [Google Scholar]
  4. Cormican M. G., Jones R. N. 1995; Use of multiplex PCR to detect and identify Mycobacterium avium and M . intracellulare in blood culture fluids of AIDS patients.. J Clin Microbiol 33:2230
    [Google Scholar]
  5. Corner L. A., Barrett R. H., Lepper A. W. D., Lewis V., Pearson C. W. 1981; A survey of mycobacteriosis of feral pigs in the Northern Territory.. Aust Vet J 57:537–542
    [Google Scholar]
  6. Cousins D. V., Evans R., Francis B. 1995; Use of BACTEC radiometric culture method and polymerase chain reaction for the rapid screening of faeces and tissues for Mycobacterium paratuberculosis.. Aust Vet J 72:458–462
    [Google Scholar]
  7. Cregan P., Yajko D. M., Ng V. L., Gonzalez P. C., Nassos P. S., Sanders C. A., Hadley W. K. 1992; Use of DNA probes to detect Mycobacterium intracellulare and ‘X’ mycobacteria among clinical isolates of Mycobacterium avium complex.. J Infect Dis 166:191–194
    [Google Scholar]
  8. Dawson D. J. 1990; Infection with Mycobacterium avium complex in Australian patients with AIDS.. Med J Aust 153:466–468
    [Google Scholar]
  9. Drake T. A., Hindler J. A., Berlin O. G. W., Bruckner D. A. 1987; Rapid identification of the Mycobacterium avium complex in culture using DNA probes.. J Clin Microbiol 25:1442–1445
    [Google Scholar]
  10. Falkinham J. O., Parker B. C., Gruft H. 1980; Epidemiology of infection by nontuberculous mycobacteria.. Am Rev Respir Dis 121:931–937
    [Google Scholar]
  11. Feizabadi M. M., Robertson I. D., Cousins D. V., Dawson D., Chew W., Gilbert G. L., Hampson D. J. 1996a; Genetic characterization of Mycobacterium avium isolated from humans and animals in Australia.. Epidemiol Infect 116:41–49
    [Google Scholar]
  12. Feizabadi M. M., Robertson I. D., Cousins D. V., Hampson D. J. 1996b; Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed field gel electrophoresis.. J Clin Microbiol 34:1136–1142
    [Google Scholar]
  13. Frothingham R., Wilson K. H. 1993; Sequence-based differentiation of strains in the Mycobacterium avium complex.. J Bacteriol 175:2818–2825
    [Google Scholar]
  14. Good R. C. 1985; Opportunistic pathogens in the genus mycobacterium.. Annu Rev Microbiol 39:347–369
    [Google Scholar]
  15. Guthertz L. S., Damsker B., Bottone E. J., Ford E. G., Midura T. F., Janda J. M. 1989; Mycobacterium avium and Mycobacterium intracellulare infections in patients with and without AIDS.. J Infect Dis 160:1037–1041
    [Google Scholar]
  16. Lévy-Frébault V. V., Thorel M.-F., Varnerot A., Gicquel B. 1989; DNA polymorphism in Mycobacterium paratuberculosis, wood pigeon mycobacteria, and related mycobacteria analyzed by field inversion gel electrophoresis.. J Clin Microbiol 27:2823–2826
    [Google Scholar]
  17. Lygren S. T., Closs O., Bercouvier H., Wayne L. G. 1986; Catalase, peroxide, and superoxide dismutases in Mycobacterium leprae and other mycobacteria studied by crossed immunoelectrophoresis and polyacrylamide gel electrophoresis.. Infect Immun 54:666–672
    [Google Scholar]
  18. Maynard Smith J., Smith N. H., O’Rourke M., Spratt B. G. 1993; How clonal are bacteria?. Proc Nutl Acad Sci USA 90:4384–4388
    [Google Scholar]
  19. Morita Y., Maruyama S., Katsube Y. 1994; Prevalence of atypical mycobacteriosis in slaughtered swine in Gunma prefecture and the serovars of the isolates.. J Vet Med Sci 56:475–479
    [Google Scholar]
  20. Nei M. 1987; Estimation of average heterozygosity and genetic distance from a small number of individuals.. Genetics 89:583–590
    [Google Scholar]
  21. Prince D. S., Peterson D. D., Steiner R. M., Gottlier J. E., Scott R., Israel H. L. 1989; Infection with Mycobacterium avium complex in patients without predisposing conditions.. N Engl J Med 321:863–868
    [Google Scholar]
  22. Raszka W. V., Skillman L. P., McEvay P. L., Robb M. L. 1995; Isolation of nontuberculous, non-avium mycobacteria from patients infected with human immunodeficiency virus.. Clin Infect Dis 20:73–76
    [Google Scholar]
  23. Reznikov M., Leggo J. H. 1972; Modification of Shaefer’s procedure for serotyping of organisms of the Mycobacterium avium–M. intracellulare–M. scrofulaceum complex.. Appl Microbiol 23:819–823
    [Google Scholar]
  24. Rogall T., Wolters J., Flohr T., Bðttger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium.. Int J Syst Bacteriol 40:323–330
    [Google Scholar]
  25. Saito H., Tomioka H., Sato K., Tasaka H., Tsukamura M., Kuze F., Asano K. 1989; Identification and partial characterisation of Mycobacterium avium and Mycobacterium intracellulare by using DNA probes.. J Clin Microbiol 27:994–997
    [Google Scholar]
  26. Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. 1990; Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare.. J Clin Microbiol 28:1691–1697
    [Google Scholar]
  27. Schaefer W. B. 1965; Serological identification and classification of the atypical mycobacteria by their agglutination.. Am Rev Respir Dis 92:37–42
    [Google Scholar]
  28. Selander R. K., Caugant D. A., Ochman H., Musser J. O., Gilmour M. N., Whittam T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.. Appl Environ Microbiol 51:873–884
    [Google Scholar]
  29. Stephens L. R. 1993; Johne’s disease; bacteriology and serology. In Australian Standard Diagnostic Techniques for Animal Diseases. Edited by L. A. Corner & M. T. Collins. Melbourne, Victoria: CSIRO for the Standing Committee on Agriculture and Resources Management..
    [Google Scholar]
  30. Thoen C. O., Karlson A. G. 1991; Tuberculosis. In Diseases of Poultry, pp.. 172–185 Edited by B. W. Clanek, H. J. Barnes, C. W. Beard, W. M. Reid & H. W. Yoder. Iowa: Iowa State University Press..
    [Google Scholar]
  31. Thoresen O. F., Saxegaard F. 1993; Comparative use of DNA probes for Mycobacterium avium and Mycobacterium intracellulare and serotyping for identification and characterization of animal isolates of the M . avium complex.. Vet Microbiol 34:83–88
    [Google Scholar]
  32. Vestal A. 1975; Procedures for the Isolation and Identification of Mycobacteria. Atlanta, USA: Training and Consultative Division Centre for Disease Control..
    [Google Scholar]
  33. Wards B. J., Collins D. M., delisle G. W. 1987; Restriction endonuclease analysis of members of the Mycobacterium avium–M. intracellulare–M. scrofulaceum serocomplex.. J Clin Microbiol 25:2309–2313
    [Google Scholar]
  34. Wasem C. F., McCarthy C. M., Murphy L. W. 1991; Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria.. J Clin Microbiol 29:264–271
    [Google Scholar]
  35. Wayne L. G., Good R. C., Tsang A., Butler R., Dawson D., Groothuis D., Gross W., Hawkins J., Kilburn J., Kubin M., Schroder K. H., Silcox V. A., Smith C., Thorel M. F., Woodley C., Yakrus M. A. 1993; Serovar determination and molecular taxonomic correlation in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum: a cooperative study of the international working group on mycobacterial taxonomy.. Int J Syst Bacteriol 43:482–489
    [Google Scholar]
  36. Wilton S., Cousins D. V. 1992; Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube.. PCR Methods Appl 1:269–273
    [Google Scholar]
  37. Wolinsky E. 1979; Nontuberculous mycobacteria and associated diseases.. Am Rev Respir Dis 119:107
    [Google Scholar]
  38. Wolinsky E. 1992; Mycobacteria other than tuberculosis.. Clin Infect Dis 15:1–12
    [Google Scholar]
  39. Yoshimura H. H., Graham D. Y. 1988; Nucleic acid hybridisation studies of mycobactin-dependent mycobacteria.. J Clin Microbiol 26:1309–1312
    [Google Scholar]
  40. Zhang Y., Mazurek G. H., Kave M. D., Eisenach K. D., Pang Y., Morphy D. T., Wallace R. J. 1992; DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology.. J Clin Microbiol 30:1551–1556
    [Google Scholar]
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