1887

Abstract

Six monoclonal antibodies (mAbs) against lipopolysaccharides (LPS) from (P1P3, 60.5), (PP2, PP6, PP8) and (BRg1) were used to examine the presence of antigenic determinants of LPS on cells. Forty-eight clinical isolates of this Gram-negative bacterium (4 canine, 3 equine, 6 porcine, 4 rabbit and 31 human) were examined. Significant cross-reactivities with the heterologous anti- and anti- mAbs were observed. The isolates also exhibited marked antigenic polymorphism. The 48 isolates could be classified in six immunogroups. Purified LPS preparations extracted from some isolates were analysed by ELISA, thin-layer chromatography, and tricine-SDS-PAGE. The results show that four main types of antigenic polymorphism of LPSs exist: (a) heterogeneity of the core, (b) presence or absence of O-chains, (c) differences in the hinge region between O-chain and core, and (d) differences in interactions of LPS with other cell-surface constituents. Smooth-type LPS molecules, detectable with mAb PP6, were more frequently observed in animal isolates (94%) than in human isolates (52%). Reverse frequencies were found with mAb 60.5 (48% of human isolates, 18% of animal isolates), which is unable to react with long-chain LPSs. This observation could be due to the general absence of some lectin-like receptor, specific to the O-chain, on human bronchoalveolar tissues.

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1997-04-01
2024-12-03
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References

  1. Amador C., Chiner E., Clape J. L., Ortiz de la Tabla V., Martinez C., Pasquau F. 1991; Pneumonia due to Bordetella bronchiseptica in a patient with AIDS.. Rev Infect Dis 13:771–772
    [Google Scholar]
  2. Bauwens J. E., Spach D. H., Schacker T. W., Mustafa M. M., Bowden R. A. 1992; Bordetella bronchiseptica pneumonia and bacteremia following bone marrow transplantation.. J Clin Microbiol 30:2474–2475
    [Google Scholar]
  3. Blom J., Hansen J. A., Poulsen F. M. 1983; Morphology of cells and hemagglutinogens of Bordetella species : resolution of substructural units in fimbriae of Bordetella pertussis.. Infect Immun 42:308–317
    [Google Scholar]
  4. Brunham R. C., Plummer F. A., Stephens R. S. 1993; Bacterial antigenic variation, host immune response, and pathogen–host coevolution.. Infect Immun 61:2273–2276
    [Google Scholar]
  5. Buggy B. P., Brosius F. C. I., Bogin R. M., Koller C. A., Schaberg D. R. 1987; Bordetella bronchiseptica pneumonia in a patient with chronic lymphocytic leukemia.. South Med J 80:1187–1189
    [Google Scholar]
  6. Caroff M., Karibian D. 1990; Several uses of isobutyric acid-ammonium hydroxide solvent in endotoxin analysis.. Appl Environ Microbiol 56:1957–1959
    [Google Scholar]
  7. Caroff M., Chaby R., Karibian D., Perry M., Deprun C., Szabo L. 1990; Variations in the carbohydrate regions of Bordetella pertussis lipopolysaccharides : electrophoretic, serological and structural features.. J Bacteriol 172:1121–1128
    [Google Scholar]
  8. Chaby R., Caroff M. 1988; Lipopolysaccharides of Bordetella pertussis endotoxin. In Pathogenesis and lmmunity in Pertussis, Edited by A. C. Wardlaw & R. Parton. Chichester: John Wiley. 247–271
    [Google Scholar]
  9. Cookson B. T., Goldman W. E. 1987; Tracheal cytotoxin: a conserved virulence determinant of all Bordetella species.. J Cell. Biochem 11B:124–127
    [Google Scholar]
  10. Deeb B. J., DiGiacomo R. F., Bernard B. L., Silbernagel S. M. 1990; Pasteurella multocida and Bordetella bronchiseptica infections in rabbits.. J Clin Microbiol 28:70–75
    [Google Scholar]
  11. Di Fabio J. L., Caroff M., Karibian D., Richards J. C., Perry M. B. 1992; Characterization of the common antigenic lipopolysaccharide O-chains produced by Bordetella bronchiseptica and Bordetella parapertussis.. FEMS Microbiol Lett 97:275–282
    [Google Scholar]
  12. Galanos C., Lüderitz O., Westphal O. 1969; A new method for the extraction of R lipopolysaccharides.. Eur J Biochem 9:245–249
    [Google Scholar]
  13. Ghosh H. K., Tranter J. 1979; Bordetella bronchicanis (bronchiseptica) infection in man : review and case report.. J Clin Patho1 32:546–548
    [Google Scholar]
  14. Gueirard P., Guiso N. 1993; Virulence of Bordetella bronchiseptica : role of adenylate cyclase-hemolysin.. Infect Immun 61:4072–4078
    [Google Scholar]
  15. Gueirard P., Weber C., Le Coustumier A., Guiso N. 1995; Human Bordetella bronchiseptica infection related to contact with infected animals: persistence of bacteria in host.. J Clin Microbiol 33:2002–2006
    [Google Scholar]
  16. Hartigan J. A. 1975; Clustering Algorithms. New York: John Wileyl.
    [Google Scholar]
  17. Johnson K. G., Perry M. B. 1976; Improved techniques for preparation of bacterial lipopolysaccharides.. Can J Microbiol 22:29–34
    [Google Scholar]
  18. Le Blay K., Caroff M., Richards, J. C., Perry M. B., Chaby R. 1994; Specific and cross-reactive monoclonal antibodies to Bordetella parapertussis and Bordetella bronchiseptica lipopolysaccharides.. Microbiology 140:2459–2465
    [Google Scholar]
  19. Le Blay K., Caroff M., Blanchard F., Perry M. B., Chaby R. 1996; Epitopes of the ordetella pertussis lipopolysaccharides as potential markers for typing of isolates with monoclonal antibodies.. Microbiology 142:971–978
    [Google Scholar]
  20. Lesse A. J., Campagnari A. A., Bittner W. E., Apicella M. A. 1990; Increased resolution of lipopolysaccharides and lipooligo saccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Immunol Methods 126:109–117
    [Google Scholar]
  21. Meis J. F. G. M., van Griethuijsen A. J. A., Muytjens H. L. 1990; Bordetella bronchiseptica bronchitis in an immunosuppressed patient.. Eur J Clin Microbiol Infect Dis 9:366–367
    [Google Scholar]
  22. Mesnard R., Guiso N., Michelet C., Sire J. M., Poiredras P., Donnio P. V., Avril J. L. 1993; Isolation of Bordetella bronchiseptica from a patient with AIDS.. Eur J Clin Microbiol Infect Dis 12:304–306
    [Google Scholar]
  23. Monack D. M., Arico B., Rappuoli R, Falkow S. 1989; Phase variants of B. bronchiseptica arise by spontaneous deletions in the vir locus.. Mol Microbiol 3:1719–1728
    [Google Scholar]
  24. Ng V. L., Boggs J. M., York M. K., Golden J. A., Hollander H., Hadley W. K. 1992; Recovery of Bordetella bronchiseptica from patients with AIDS.. Clin Infect Dis 15:376–377
    [Google Scholar]
  25. Novotny P., Kobish M., Cownley K., Chubb A. P., Montaraz J. A. 1985; Evaluation of Bordetella bronchiseptica vaccines in specific-pathogen-free piglets with bacterial cell surface antigens in enzyme-linked immunosorbent assay.. Infect Immun 50:190–198
    [Google Scholar]
  26. Potter J. F., Parton R., Wardlaw A. C. 1991; Growth and survival of Bordetella bronchiseptica in natural waters and in buffered saline without added nutrients.. Appl Environ Microbiol 5:1202–1206
    [Google Scholar]
  27. Potter J. F., Connor K., van der Zee A., Reubsaet F., Ibsen P., Heron I., Chaby R., Le Blay K., Donachie W. 1995; Characterisation of ovine Bordetella parapertussis isolates by analysis of specific endotoxin (lipopolysaccharide) epitopes, filamentous haemagglutinin production, cellular fatty acid composition and antibiotic sensitivity.. FEMS Microbiol Lett 132:195–201
    [Google Scholar]
  28. Regan J., Lowe F. 1997; Enrichment medium for the isolation of Bordetella. . J Clin Microbiol 6:303–309
    [Google Scholar]
  29. Rudel T., van Putten J. P. M., Gibbs C. P., Haas R., Meyer T. F. 1992; Interaction of two variable proteins (PilE and PilC) required for pilus-mediated adherence of Neisseria gonorrhoeae to human epithelial cells.. Mol Microbiol 6:3439–3450
    [Google Scholar]
  30. Schägger H., VonJagow G. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.. Anal Biochem 166:368–379
    [Google Scholar]
  31. Stoll D. B., Murphey S. A., Ballas S. K. 1981; Bordetella bronchiseptica infection in stage IV Hodgkin’s disease. Postgrad Med J 57:723–724
    [Google Scholar]
  32. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.. Anal Biochem 119:115–119
    [Google Scholar]
  33. Tuomanen E. I., Nedelman J., Hendley J. O., Hewlett E. L. 1983; Species specificity of Bordetella adherence to human and animal ciliated respiratory epithelial cells.. Infect Immun 42:692–695
    [Google Scholar]
  34. Woolfrey B. F., Moody J. A. 1991; Human infections associated with Bordetella bronchiseptica.. Clin Microbiol Rev 4:243–255
    [Google Scholar]
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