1887

Abstract

(now is a symbiotic nitrogen-fixing bacterium that can nodulate soybean in a cultivar-specific manner. This process is governed by a set of negatively acting nodulation genes termed These genes prevent strain USDA257 from infecting soybean cultivars such as McCall, but they do not block nodulation of cultivar Peking. strain USDA191 contains DNA sequences that hybridize to yet it forms normal nitrogen-fixing nodules on both McCall and Peking soybean. These sequences were isolated and their structure and function examined in comparison to of strain USDA257. Restriction maps of the two loci are identical, as is a 2∙4 kb DNA sequence that corresponds to and its promoter region. Expression of by strain USDA191 is flavonoid-dependent in culture and readily detectable in nodules. The gene is not inducible in a mutant of strain USDA191 that lacks the regulatory gene, and its expression is greatly attenuated in a mutant. is also present and flavonoid-inducible in HH103, a second strain that nodulates McCall soybean normally. Inactivation of in strain HH103, USDA191 or USDA257 leads to retardation of initial nodulation rates on soybean cultivars such as Peking and to acquisition of the capacity to form nitrogen-fixing nodules on two species of is thus of symbiotic significance in all three strains, even though it regulates soybean cultivar specificity only in strain USDA257.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-4-1381
1997-04-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/4/mic-143-4-1381.html?itemId=/content/journal/micro/10.1099/00221287-143-4-1381&mimeType=html&fmt=ahah

References

  1. Appelbaum E. R., Thompson D. V., Idler K., Chartrain N. 1988; Rbizobium japonicum USD A 191 has two nodD genes that differ in primary structure and function.. J Bacteriol 170:12–20
    [Google Scholar]
  2. Balatti P. A., Pueppke S.G. 1992; Identification of North American soybean lines that form nitrogen-fixing nodules with Rhizobium fredii USDA257.. Can J Plant Sci 72:49–55
    [Google Scholar]
  3. Balatti P. A., Kovcics L. G., Krishnan H. B., Pueppke S. G. 1995; Rhizobium sp. NGR234 contains a functional copy of the soybean cultivar specificity locus, nolXWBTUV.. Mol Plant–Microbe Interact 8:693–699
    [Google Scholar]
  4. Bec-Ferté M.-P., Krishnan H. B., Promé D., Savagnac A., Pueppke S.G., Promé J.-C. 1994; Structures of nodulation factors from the nitrogen-fixing soybean symbiont Rhizobium fredii USDA257.. Biochemistry 33:11782–11788
    [Google Scholar]
  5. Boivin C., Camut S., Malpica C. A., Truchet G., Rosenberg C. 1990; Rhizobium meliloti genes encoding catabolism of trigonelline are induced under symbiotic conditions.. Plant Cell 2:1157–1170
    [Google Scholar]
  6. Boundy-Mills K. L., Kosslak R. M., Tully R. E., Pueppke S. G., Lohrke S., Sadowsky M. J. 1994; Induction of the Rhizobium fredii nod box-independent nodulation gene nolj requires a functional nodD1 gene.. Mol Plant-Microbe Interact 7:305–308
    [Google Scholar]
  7. Buendía-Clavería A. M., Chamber M., Ruiz-Sainz J. E. 1989; A comparative study of the physiological characteristics, plasmid content and symbiotic properties of different Rhizobium fredii strains.. Syst Appl Microbiol 12:203–209
    [Google Scholar]
  8. Chatterjee A., Balatti P. A., Gibbons W., Pueppke S. G. 1990; Interaction of Rhizobium fredii and nodulation mutants derived from it with the agronomically improved soybean cultivar McCall.. Planta 180:303–311
    [Google Scholar]
  9. Dowdle S. F., Bohlool B. B. 1985; Predominance of fastgrowing Rhizobium japonicum in a soybean field in the People’s Republic of China.. Appl Environ Microbiol 50:1171–1176
    [Google Scholar]
  10. Fisher R. F., Long S. R. 1993; Interactions of NodD at the nod box: NodD binds to two distinct sites on the same face of the helix and induces a bend in the DNA.. J Mol Biol 233:336–348
    [Google Scholar]
  11. Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. 1982; Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rbizobium mutants.. Gene 18:289–296
    [Google Scholar]
  12. Harlow E., Lane D. 1988; Antibodies, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  13. Heron D. S., Pueppke S.G. 1984; Mode of infection, nodulation specificity, and indigenous plasmids of 11 fastgrowing Rhizobium japonicum strains.. J Bacteriol 160:1061–1066
    [Google Scholar]
  14. Heron D. S., Érsek T., Krishnan H. B., Pueppke S. G. 1989; Nodulation mutants of Rhizobium fredii USDA257.. Mol Plant-Microbe Interact 2:4–10
    [Google Scholar]
  15. Keen N. T., Tamaki S., Kobayashi G., Trollinger D. 1988; Improved broad-host-range plasmids for DNA cloning in Gramnegative bacteria.. Mol Plant-Microbe Interact 70:190–197
    [Google Scholar]
  16. Keyser H. H., Bohlool B. B., Hu T. S., Weber D. F. 1982; Fastgrowing rhizobia isolated from root nodules of soybean.. Science 215:1631–1632
    [Google Scholar]
  17. Kosslak R. M., Bookland R., Barkei J., Paaren H. E., Appelbaum E. R. 1987; Induction of Bradyrhizobium japonicum common nod genes by isoflavones isolated from Glycine max.. Proc Natl Acad Sci USA 84:7428–7432
    [Google Scholar]
  18. Kovács L. G., Balatti P. A., Krishnan H. B., Pueppke S. G. 1995; Transcriptional organization and expression of nolXWBTUV, a locus that regulates cultivar-specific nodulation of soybean by Rhizobium fredii USDA257.. Mol Microbiol 17:923–933
    [Google Scholar]
  19. Krishnan H. B., Pueppke S. G. 1991; Sequence and analysis of the nodABC region of Rhizobium fredii USDA257, a nitrogenfixing symbiont of soybean and other legumes.. Mol Plant-Microbe Interact 4:512–520
    [Google Scholar]
  20. Krishnan H. B., Pueppke S. G. 1993; Flavonoid inducers of nodulation genes stimulate Rhizobium fredii USDA257 to export proteins into the environment.. Mol Plant-Microbe Interact 6:107–113
    [Google Scholar]
  21. Krishnan H. B., Pueppke S. G. 1994a; Cultivar-specificity genes of the nitrogen-fixing soybean symbiont, Rhizobium fredii USDA257, also regulate nodulation of Erythrina spp.. Am J Bot 81:38–45
    [Google Scholar]
  22. Krishnan H. B., Pueppke S. G. 1994b; Host range, RFLP, and antigenic relationships between Rhizobium fredii strains and Rhizobium sp. NGR234.. Plant Soil 161:21–29
    [Google Scholar]
  23. Krishnan H. B., Kuo C.-L., Pueppke S. G. 1995; Elaboration of flavonoid-induced proteins by the nitrogen-fixing soybean symbiont Rhizobium fredii is regulated by both nodD1 and nodD2, and is dependent on the cultivar-specificity locus, nolXWBTUV.. Microbiology 141:2245–2251
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  25. Martinez E., Romero D., Palacios R. 1990; The Rhizobium genome.. Crit Rev Plant Sci 9:59–93
    [Google Scholar]
  26. Meinhardt L. W., Krishnan H. B., Balatti P. A., Pueppke S. G. 1993; Molecular cloning and characterization of a sym plasmid locus that regulates cultivar-specific nodulation of soybean by Rhizobium fredii USDA257.. Mol Microbiol 9:17–29
    [Google Scholar]
  27. Miller J. H. 1972; Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  28. Preston G., Huang H.-C., He S. Y., Collmer A. 1995; The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and tomato are encoded by an operon containing Yersinia ysc homologs and elicit the hypersensitive response in tomato but not soybean.. Mol Plant-Microbe Interact 8:717–732
    [Google Scholar]
  29. Pueppke S.G. 1996; The genetic and biochemical basis for nodulation of legumes by rhizobia.. Crit Rev Biotechnol 16:1–51
    [Google Scholar]
  30. Rodriguez-Navarro D. N., Ruiz-Sainz J. E., Buendia-Claveria A. M., Santamaria C., Balatti P. A., Krishnan H. B., Pueppke S. G. 1996; Characterization of rhizobia from nodulated soybean [Glycine max (L.)Merr.] growing in Vietnam.. Syst Appl Microbiol 19:240–248
    [Google Scholar]
  31. Salmond G. P. 1994; Secretion of extracellular virulence factors by plant pathogenic bacteria.. Annu Rev Phytopathol 32:181–200
    [Google Scholar]
  32. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.. Annu Rev Phytopathol
    [Google Scholar]
  33. Schlaman H. R. M., Okker R. J. H., Lugtenberg B. J. J. 1992; Regulation of nodulation gene expression by NodD in rhizobia.. J Bacteriol 174:5177–5182
    [Google Scholar]
  34. Scott-Craig J. S., Guerinot M. L., Chelm B. K. 1991; Isolation of Bradyrhizobium japonicum DNA sequences that are transcribed at high levels in bacteroids.. Mol Gen Genet 228:356–360
    [Google Scholar]
  35. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria.. Bio/Technology 1:784–791
    [Google Scholar]
  36. Van Gijsegem F, Gough C., Zischek C., Niqueux E., Arlat M., Genin S., Barberis P., German S., Castello P., Boucher C. 1995; The hrp gene locus of Pseudomonas solanacearum, which controls the production of a type III secretion system, encodes eight proteins related to components of the bacterial flagellar biogenesis complex.. Mol Microbiol 15:1095–1114
    [Google Scholar]
  37. Vincent J. M. 1970; A Manual for the Practical Study of Root-Nodule Bacteria. Oxford: Blackwell Scientific..
    [Google Scholar]
  38. Wang S., Stacey G. 1991; Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod-box.. 173:3356–3365
    [Google Scholar]
  39. Zylicz M., Georgopoulos G. 1984; Purification and properties of the Escherichia coli dnaK replication protein.. 259:8820–8825
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-4-1381
Loading
/content/journal/micro/10.1099/00221287-143-4-1381
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error